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Tabatabaei S.,Ramin Khuzestan Agricultural and Natural Resources University
Journal of Animal and Veterinary Advances | Year: 2010

The purpose of this study was to evaluate tshe optimum number of spermatozoa for highest fertility in Ross-308 broiler breeder chicken. About 24 Ross hens in 4 treatment groups were used. Semen was collected from 8 Ross roosters by abdominal massage and pooled in glass tube. After initially dilution with modified Ringer's solution and determination the spermatozoa concentration by hemocytometer, the semen was diluted further. About 50, 75, 100 and 150 million spermatozoa in 0.1 mL were inseminated to treatment groups for 4 weeks (4 times repeating). Eggs were collected daily from the 2nd day post insemination and incubated. After 7 days of incubation, the eggs were candled for fertility rate. In treatment groups that were inseminated with 50, 75, 100 and 150 million spermatozoa, fertility rates were 52.85±4.15, 72.37±5.28, 87.64±4.81 and 89.12±3.74%, respectively. As shown to this results, fertility rate in treatment group that was inseminated with 50 million spermatozoa was lowest (52.85±4.15%) and then increased significantly (p<0.05) with increasing the sperm number and reached to maximum in treatment group that was inseminated with 100 million spermatozoa (87.64±4.81%). Difference of fertility rate between treatment groups that were inseminated with 100 and 150 millionspermatozoa (87.64±4.81 and 89.12±.74%, respectively) was not significant (p>0.05). It is concluded that optimum dose of spermatozoa for artificial insemination in Ross-308 chicken was 100 million sperm. © Medwell Journals, 2010.


Mohammadabadi T.,Ramin Khuzestan Agricultural and Natural Resources University | Chaji M.,Ramin Khuzestan Agricultural and Natural Resources University
Journal of Applied Animal Research | Year: 2011

To investigate the effect of exogenous enzymes on fermentation and in vitro rumen anaerobic bacterial growth, sesame straw (SS) was treated with 0, 1, 3 and 5 g/kg exogenous enzyme. Enzyme increased disappearance of dry matter and neutral detergent fibre of SS by bacteria in a dose dependent manner (P<0.05). Highest level of enzyme resulted in the highest gas production, metabolisable energy, organic matter digestibility, ammonia-N and short chain fatty acid concentrations. It may be concluded that the growth and activity of rumen anaerobic bacteria on SS is favourably influenced by exogenous enzyme content. © 2011 Taylor & Francis.


Aghaei A.,Ramin Khuzestan Agricultural and Natural Resources University | Tabatabaei S.,Ramin Khuzestan Agricultural and Natural Resources University | Nazari M.,Ramin Khuzestan Agricultural and Natural Resources University
Journal of Animal and Veterinary Advances | Year: 2010

The aim of this study was to evaluate the correlation between cooper, zinc, calcium, sodium and potassium concentration of seminal plasma and spermatozoa progressive motility percent in rooster. Seventeen Indigenous broiler breeder Roosters were used. According to spermatozoa motility, roosters were classified to 3 treatment groups with low, medium and high progressive motility percent. Seminal plasma from all groups was obtained with centrifuge of semen samples. Cooper, Zinc, Sodium, potassium and calcium concentrations in seminal plasma samples were determined on the atomic absorption spectrophotometer. There was positive correlation between cooper and zinc concentrations of seminal plasma and progressive motility percent of spermatozoa. There was no significant correlation between sodium concentrations of seminal plasma and progressive motility percent of spermatozoa. There was negative correlation between low/medium progressive motility rate groups and high motility group. While this correlation for calcium concentration was positive. © Medwell Journals, 2010.


Tabatabaei S.,Ramin Khuzestan Agricultural and Natural Resources University | Mamoei M.,Ramin Khuzestan Agricultural and Natural Resources University | Aghaei A.,Ramin Khuzestan Agricultural and Natural Resources University
Comparative Clinical Pathology | Year: 2011

In the present study, ovarian follicular fluid metabolites, ions, enzymes and hormonal profiles in relation to follicular size in Holstein cattles were investigated. Fluid from small (4-5 mm), medium (6-9 mm) and large (10-20 mm) follicles were collected from 80 clinically healthy and non-pregnant adult cattles. Follicular fluid samples were analyzed for iron, calcium, phosphorous, sodium, potassium, magnesium, glucose, urea, creatinine, cholesterol, triglyceride, protein, albumin, globulin, alkaline phosphatase (ALK), lactate dehydrogenase (LDH), aspartate aminotransferase (ASAT), alanine aminotransferase (ALAT), progesterone, testosterone, estradiol-17β, T3 and T4. The concentration of urea, creatinine and protein in small, medium and large follicles did not differ (P<0.05). The concentrations of phosphorus, magnesium, cholesterol, triglyceride and albumin in follicular fluid decreased (P<0.05) with the development of the follicle while the reverse was true for calcium, sodium, globulin and estradiol-17β concentrations (P<0.05). Glucose concentration in small follicles was lower (P<0.05) than that in medium and large follicles. The concentrations of iron, ALK, LDH, ALAT and ASAT were higher (P<0.05) than in small follicles. The concentrations of potassium, progesterone and T3 in large follicles were lower (P<0.05) than those in small and medium follicles while the reverse was true for the T4 concentration (P<0.05). © 2010 Springer-Verlag London Limited.


Tabatabaei S.,Ramin Khuzestan Agricultural and Natural Resources University | Mamoei M.,Ramin Khuzestan Agricultural and Natural Resources University
Comparative Clinical Pathology | Year: 2011

The aim of this study was to examine the biochemical composition of follicular fluid from different-sized follicles and its relationship with that of blood plasma in buffalo. Ovaries of adult and healthy buffaloes were collected after slaughter. Follicular fluid was aspirated from three size classes of follicles (4-5, 6-9, and 10-20 mm diameter). Blood samples were also collected from these buffaloes immediately before slaughter. The follicular fluid and blood plasma samples were analyzed for metabolites (glucose, total protein, albumin, globulin, cholesterol, triglycerides, urea, and creatinine), ions (calcium and phosphorus), and enzymes (alkaline phosphatase, aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase). The follicular fluid calcium, urea, creatinine, albumin, and ASAT and ALAT concentrations were not affected by the size of the ovarian follicles. Follicular fluid concentrations of phosphorus, cholesterol, triglyceride, ALK, and LDH decreased from small to large follicles. Phosphorus, albumin, and LDH concentrations in plasma were significantly lower than the levels in all follicle classes. The plasma concentrations of glucose, creatinin, cholesterol, triglyceride, protein, and glubolin were higher than in the small, medium, and large follicles. The concentration of glucose in the small follicles was significantly lower than in the medium and large follicles. Total protein concentration in fluid of small follicles was significantly higher than in the large follicles. The amount of globulin in medium follicles was higher than in the small and large follicles. The plasma concentration of ALK was significantly lower than in the small and medium follicles. ALK concentration difference between large follicles and plasma was not significant. © 2010 Springer-Verlag London Limited.

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