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Emmeloord, Netherlands

Ospina C.A.,Wageningen University | Lammerts van Bueren E.T.,Wageningen University | Allefs J.J.H.M.,Agrico Research | Engel B.,Wageningen University | And 3 more authors.

Potato (Solanum tuberosum L.) requires abundant nitrogen (N) to perform well and has low nitrogen use efficiency (NUE). We assessed phenotypic variation among 189 potato cultivars for NUE and the association between NUE and ecophysiological variables describing canopy development (CDv), under high and low N input. In 2009 and 2010, 189 cultivars were grown with N supply (soil N + fertiliser N) of 75 or 180 kg N/ha at Bant, the Netherlands. CDv was assessed weekly as the percentage of soil covered by green potato leaves (%SC). Data were analysed using a model that described CDv as a function of thermal time, based on the Beta function and estimates of cardinal temperatures. Nitrogen significantly affected model-derived, biologically relevant, curve-fit parameters for each cultivar. The t 1 (i.e., thermal time required to reach maximum soil cover (Vx)) was higher at low than at high N. Other parameters were higher at high than at low N, especially Vx and the period over which it was maintained. Nitrogen also affected tuber dry matter yield, tuber size and weight distributions, N content and N uptake but not tuber dry matter percentage. The total area under the %SC curve was highly correlated with yield in both years. Cultivars performing well under high N also performed well under low N. There was large variation in NUE component traits among cultivars; maturity type partially explained this variation. Variables of the CDv model captured this variation, N effects on light interception and its correlation with yield. © 2014 Springer Science+Business Media Dordrecht. Source

Juhasz Z.,Agricultural Biotechnology Center | Dancs G.,Agricultural Biotechnology Center | Marincs F.,Agricultural Biotechnology Center | Vossen M.,Agrico Research | And 2 more authors.
Plant Breeding

Vitamin C, B5, and B6 contents of potato tubers were measured by gas chromatography-mass spectrometry (GC-MS). Two diploid potato populations derived from two different crosses were analyzed. Plants were grown at two locations under different environmental conditions. Measurements of vitamin content of tubers showed high variation in both populations. A weak correlation was found between the vitamin concentrations of tubers harvested at the two locations. Correlations between the mean values of vitamins in field-grown tubers and in different tissues derived from in vitro or greenhouse-grown potato plants were also determined. A very high correlation between the vitamin content of field- and greenhouse-grown tubers, and between field-grown tubers and sink leaves of greenhouse-grown plants was detected. This finding can facilitate breeding by preselection of individuals with improved vitamin content under greenhouse conditions at their early developmental stage. © 2014 Blackwell Verlag GmbH. Source

Bakker E.,Wageningen University | Bakker E.,Center for Biosystems Genomics | Borm T.,Wageningen University | Prins P.,Wageningen University | And 26 more authors.
Theoretical and Applied Genetics

Like all plants, potato has evolved a surveillance system consisting of a large array of genes encoding for immune receptors that confer resistance to pathogens and pests. The majority of these so-called resistance or R proteins belong to the super-family that harbour a nucleotide binding and a leucine-rich-repeat domain (NB-LRR). Here, sequence information of the conserved NB domain was used to investigate the genome-wide genetic distribution of the NB-LRR resistance gene loci in potato. We analysed the sequences of 288 unique BAC clones selected using filter hybridisation screening of a BAC library of the diploid potato clone RH89-039-16 (S. tuberosum ssp. tuberosum) and a physical map of this BAC library. This resulted in the identification of 738 partial and full-length NB-LRR sequences. Based on homology of these sequences with known resistance genes, 280 and 448 sequences were classified as TIR-NB-LRR (TNL) and CC-NB-LRR (CNL) sequences, respectively. Genetic mapping revealed the presence of 15 TNL and 32 CNL loci. Thirty-six are novel, while three TNL loci and eight CNL loci are syntenic with previously identified functional resistance genes. The genetic map was complemented with 68 universal CAPS markers and 82 disease resistance trait loci described in literature, providing an excellent template for genetic studies and applied research in potato. © 2011 The Author(s). Source

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