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Faisalābād, Pakistan

Rahman S.U.,Agri Biotechnology Research Institute | Malik T.A.,University of Agriculture at Faisalabad | Malik S.T.,University of Agriculture at Faisalabad
Journal of Animal and Plant Sciences

Small hairs on leaves and stems also known as trichomes are biologically an important insect resistant trait in cotton. Variations in form, function and distribution of hairs within a species have been exploited for developing insect resistant cotton cultivars. The DNA marker for the velvet hairiness trait was identified by RAPD technique using bulked segregant analysis. A cross of velvet hairy (densely hairy) and sparsely hairy genotypes was made and segregating F2 generation was developed for DNA marker studies. Out of the 320 RAPD primers used, 36 showed polymorphism between the parents. The primer, GLG-6975 showed tight linkage with velvet hairy trait in the F2 population. This RAPD marker may be used in molecular breeding of cotton for insect resistance. Source

Shaheen M.,Bahauddin Zakariya University | Murtaza N.,Bahauddin Zakariya University | Qayyum A.,Bahauddin Zakariya University | Malik W.,Bahauddin Zakariya University | And 4 more authors.
Journal of Food, Agriculture and Environment

Selection and evaluation of nineteen potato (Solanum tuberosum L.) genotypes for yield and yield components by calculating genotypic, phenotypic correlation coefficient, heritability and co-heritability was done at Potato Research Sub-Station Muree. The positive genotypic correlation coefficient was observed for different potato grades, number of eyes/tuber and plant height with yield. The amount of co-heritability and broad sense heritability was maximum for traits, plant height, <35 mm potato grade and 35-55 mm potato grade indicated preponderance of strong genetic expression. Therefore, there was a potential for the concurrent genetic improvement of these traits through selection and breeding in the plant material used in present study. Source

Qureshi M.A.,Agri Biotechnology Research Institute | Iqbal A.,Agri Biotechnology Research Institute | Akhtar N.,Agri Biotechnology Research Institute | Shakir M.A.,Agri Biotechnology Research Institute | Khan A.,Institute of Soil Chemistry and Environmental science
Soil and Environment

The development of symbiotic relationship between rhizobia and leguminous plants results in active nitrogen fixation and plays a marvelous role in agriculture systems. L-tryptophan (L-TRP) is an amino acid that acts as physiological precursor of auxins and is responsible for biosynthesis of auxins in the rhizosphere. Pot experiment was conducted at the Soil Bacteriology Section Faisalabad to assess the co-inoculation effect of N2-fixing (Rhizobium) and P-solubilizing (Bacillus sp) in the presence of L-TRP. Results revealed that co-inoculation improved the pod and straw yield considerably but the effect was more pronounced with L-TRP. Co-inoculation increased the root length, root mass, number of nodule and mass as compared to control with L-TRP. Co-inoculation with L-TRP produced 30.87 pod and 32.73 g pot-1 straw yield followed by 30.47 and 31.10 g pot-1 with rhizobial inoculation, respectively. Co-inoculation produced higher root mass (33.5 g), root length (36.0 cm), nodule number (34), nodule mass (0.131 g) and these values were further enhanced with L-TRP (40.5 g, 49 cm, 48 and 0.145 g). Co-inoculation enhanced the nutrient concentration in mash plant, grains and improved the nodulation as compared to the separate bacterial inoculations. Co-inoculation with L-TRP produced higher soil N and available P in post harvest soil samples as compared to control. Study demonstrated that co-inoculation of Rhizobium and Bacillus species influenced the yield components positively than their separate inoculation and this effect could be more assenting with L-TRP. However, the approach of precursor-inocula interaction should be studied more comprehensively in different ecological zones to sustain the crop yield. © 2012, Soil Science Society of Pakistan. Source

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