Agresearch Ltd. | Date: 2016-10-20
The invention relates to a system and method for processing of fibres and particulates such as wool, wherein material is placed within a receptacle configured to allow the ingress and egress of treatment fluid, conveyed to a treatment fluid application area, treated with a treatment fluid, and conveyed from the treatment fluid application area.
Agresearch Ltd. | Date: 2017-09-13
The invention provides methods and materials for increasing root biomass in a plant, by increasing the expression of at least one PEAPOD protein, or fragment thereof, in the plant. The invention also provides methods and materials for producing a plant increased root biomass, the method comprising the step of increasing the expression of at least one PEAPOD protein, or fragment thereof, in the plant.
Agresearch Ltd. | Date: 2017-09-13
The invention provides methods and materials for increasing at least one of root biomass and above-ground biomass and in a Poaceae plant by expressing a PEAPOD protein, or fragment thereof, in the Poaceae plant. The invention also provides methods and materials producing a Poaceae plant with at least one of increased root biomass and increased above-ground biomass, by expressing a PEAPOD protein, or fragment thereof, in the Poaceae plant.
Munday R.,Agresearch Ltd.
Toxicon | Year: 2011
Palytoxin and its derivatives have been implicated in toxic events in humans following ingestion or inhalation, and many studies on the toxicities of these substances to animals, via various routes of administration, have been described. In this report, the toxicity of palytoxin to animals has been reviewed, with comments on possible mechanisms of action. Information required for the risk assessment of palytoxin and its derivatives is by no means complete, and recommendations for further studies, which may better inform regulatory decisions regarding these substances, are also discussed. © 2010 Elsevier Ltd.
Henderson G.,Agresearch Ltd.
PloS one | Year: 2013
Molecular microbial ecology techniques are widely used to study the composition of the rumen microbiota and to increase understanding of the roles they play. Therefore, sampling and DNA extraction methods that result in adequate yields of microbial DNA that also accurately represents the microbial community are crucial. Fifteen different methods were used to extract DNA from cow and sheep rumen samples. The DNA yield and quality, and its suitability for downstream PCR amplifications varied considerably, depending on the DNA extraction method used. DNA extracts from nine extraction methods that passed these first quality criteria were evaluated further by quantitative PCR enumeration of microbial marker loci. Absolute microbial numbers, determined on the same rumen samples, differed by more than 100-fold, depending on the DNA extraction method used. The apparent compositions of the archaeal, bacterial, ciliate protozoal, and fungal communities in identical rumen samples were assessed using 454 Titanium pyrosequencing. Significant differences in microbial community composition were observed between extraction methods, for example in the relative abundances of members of the phyla Bacteroidetes and Firmicutes. Microbial communities in parallel samples collected from cows by oral stomach-tubing or through a rumen fistula, and in liquid and solid rumen digesta fractions, were compared using one of the DNA extraction methods. Community representations were generally similar, regardless of the rumen sampling technique used, but significant differences in the abundances of some microbial taxa such as the Clostridiales and the Methanobrevibacter ruminantium clade were observed. The apparent microbial community composition differed between rumen sample fractions, and Prevotellaceae were most abundant in the liquid fraction. DNA extraction methods that involved phenol-chloroform extraction and mechanical lysis steps tended to be more comparable. However, comparison of data from studies in which different sampling techniques, different rumen sample fractions or different DNA extraction methods were used should be avoided.
Janssen P.H.,Agresearch Ltd.
Animal Feed Science and Technology | Year: 2010
A descriptive model is presented that can explain changes in the amount of methane (CH4) formed in the rumen in relation to passage rate, feed type, and the effects of pH and inhibitors of methanogenesis. The model is based on methanogen growth kinetics in continuous systems. The growth rate of hydrogen (H2) utilising methanogens in the rumen and the prevailing H2 concentration are dynamically linked. Higher H2 concentrations are required to permit a growth rate sufficient to prevent washout of methanogens from the rumen at higher ruminal passage rates, at suboptimal ruminal pH values, or in the presence of inhibitors. Lower H2 concentrations are possible when the passage rate is lower, when the pH is near optimum, or when methanogens are less affected by inhibitors. Analysis of the literature confirms that increased particulate passage rate is associated with higher rumen H2 concentrations, less CH4 formation, and increased importance of propionate as a fermentation endproduct. Published data also show that partial inhibition of methanogens results in higher H2 concentrations, less CH4 formation, and more propionate formation. The model suggests that the prevailing H2 concentration influences the thermodynamics of rumen fermentation. H2 producing fermentation pathways are favoured at low H2 concentrations. Therefore, feeds and conditions that result in low H2 partial pressures will result in more H2 formation, and less propionate formation, and so more CH4 is formed per mole of feed monomer fermented in the rumen. Conversely, feeds and additives that favour high H2 concentrations result in less H2 formation per mole of feed monomer fermented in the rumen, and so result in production of less CH4 and more propionate. © 2010 Elsevier B.V.
Agresearch Ltd. | Date: 2014-04-17
The invention provides a method for encapsulating a protein of interest, the method comprising the step of expressing a fusion protein comprising an N-terminal region of a rearrangement hot spot (RHS)-repeat-containing protein fused to the protein of interest. The invention further provides applications for the encapsulation, release and delivery of the protein of interest. The invention also encompasses the encapsulated protein of interest and compositions comprising the encapsulated protein of interest. The invention also provides uses of the encapsulated protein of interest, optionally after release from encapsulation, to control pests. The encapsulated protein of interest may for example be produced via expression in a plant to control a pest of the plant, such as an insect pest.
Agresearch Ltd. | Date: 2014-03-13
A method of altering the composition of animal waste including introducing internally to an animal one or more treatment substances that can directly or indirectly affect the conversion of nitrogen containing compounds in animal waste, such that animal waste acts as a carrier so that the one or more substances affect the conversion of nitrogen containing compounds once the waste has been excreted from the animal.
Agresearch Ltd. | Date: 2015-04-30
Provided are methods of reducing milk somatic cell count using L-arginine supplementation of lactating ruminant animals during gestation, and/or during the lactation phase post parturition to decrease somatic cell count in milk produced by the animals.
Agresearch Ltd. | Date: 2015-07-02
The present invention relates to a novel bacterium Yersinia entomophaga MH96 as deposited at DSMZ on 4 May 2006 and designated accession no. DSM 18238. The present invention also relates to substances obtained or derived from Yersinia entomophaga MH96, which retain biopesticide activity. Methods for protecting a plant from pest infestation which include applying to the plant or its environment an effective amount of Yersinia entomophaga MH96 or a product delivered from the bacterium are also described.