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Bolukbas S.C.,Ataturk University | Al-sagan A.A.,King Abdulaziz City for Science and Technology | Urusan H.,Ataturk University | Erhan M.K.,Agr Ibrahim Cecen University | And 2 more authors.
Journal of Animal Physiology and Animal Nutrition | Year: 2016

This study was conducted to determine the effects of dietary cerium oxide levels (0, 100, 200, 300 or 400 mg/kg) on the laying performance, egg quality, some blood serum parameters and egg lipid peroxidation of laying hen. In total, one hundred and twenty 22-week-old brown Lohman LSL laying hens were randomly assigned to five groups equally (n = 24). Each treatment was replicated six times. Dietary supplementation of cerium oxide had no significant effect on feed intake and egg weight. The addition of cerium oxide to the laying hens' feed improved feed conversion ratio and increased (p < 0.05) egg production. Quality criteria of egg for except shell breaking strength were not affected by supplementing cerium oxide. In particular, supplementation of 200 and 300 mg/kg cerium oxide to the laying hens feed led to a significant (p < 0. 01) increase in egg shell breaking strength. Calcium and phosphorus concentration of serum increased significantly (p < 0.05) with supplementation of 100 mg/kg cerium oxide to laying hen diets. It was also observed that serum superoxide dismutase (SOD) activity and malondialdehyde (MDA) concentration decreased significantly with supplementation of cerium oxide in diets. Inclusion of cerium oxide resulted in a significant reduction in thiobarbituric acid reactive substance (TBARS) values in egg yolk in this study. It can be concluded that the addition of cerium oxide had positive effects on egg production, feed conversion ratio and egg shelf life. Based on the results of this study, it could be advised to supplement laying hens feed with cerium oxide as feed additives. Journal of Animal Physiology and Animal Nutrition © 2016 Blackwell Verlag GmbH

Genisel M.,Agr Ibrahim Cecen University | Turk H.,Ataturk University | Erdal S.,Ataturk University | Demir Y.,Ataturk University | And 2 more authors.
Turkish Journal of Botany | Year: 2015

In the present study, to determine the effects of β-estradiol on the ability of plants to tolerate lead toxicity, β-estradiol (10 μM) and lead (1.75 mM), singly or in combination, were exogenously applied to wheat seeds. Although lead resulted in a marked increase in the activities of antioxidant enzymes, including superoxide dismutase, guaiacol peroxidase, ascorbate peroxidase, and glutathione reductase (but not catalase), as well as an increase in the level of antioxidant compounds such as ascorbic acid and glutathione, this was insufficient to ameliorate the lead-induced oxidative injury or the superoxide anion, hydrogen peroxide, and malondialdehyde levels. However, β-estradiol was able to reduce the lead-induced oxidative damage and improved the antioxidant system. Similarly, β-estradiol reduced lead-induced α-amylase activity. The effects of lead toxicity on genetic material were also determined using the randomly amplified polymorphic DNA technique. While lead led to DNA damage in wheat seedlings, β-estradiol significantly mitigated this damage. Our element analysis results show that β-estradiol did not prevent lead uptake by roots, even it did stimulate the accumulation there. Taken together, our data demonstrate for the first time that β-estradiol-induced lead tolerance is associated with many biochemical and molecular mechanisms, including the antioxidant system, detoxification of reactive oxygen species, modulation of uptake and accumulation of lead, and protection of genetic material. © TÜBİTAK.

Erturk F.A.,Eurasian University | Agar G.,Ataturk University | Arslan E.,Ataturk University | Nardemir G.,Agr Ibrahim Cecen University
Environmental Science and Pollution Research | Year: 2015

Conditions of environmental stress are known to lead genetic and epigenetic variability in plants. DNA methylation is one of the important epigenetic mechanisms and plays a critical role in epigenetic control of gene expression. Thus, the aim of the study was to investigate the alteration of genome methylation induced by zinc stress by using coupled restriction enzyme digestion-random amplification (CRED-RA) technique in maize (Zea mays L.) seedlings. In addition, to determine the effect of zinc on mitotic activity and phytohormone level, high-pressure liquid chromatography (HPLC) and mitotic index analysis were utilized. According to the results, mitotic index decreased in all concentrations of zinc except for 5 mM dose and chromosome aberrations such as c-mitosis, stickiness, and anaphase bridges were determined. It was also observed that increasing concentrations of zinc caused an increase in methylation patterns and decrease in gibberellic acid (GA), zeatin (ZA), and indole acetic acid (IAA) levels in contrast to abscisic acid (ABA) level. Especially increasing of ABA levels under zinc stress may be a part of the defense system against heavy metal accumulation in plants. © 2015, Springer-Verlag Berlin Heidelberg.

Erdem M.,Frat University | Duran H.,Agr Ibrahim Cecen University | Sahin M.,Frat University | Ozdemir I.,Frat University
Desalination and Water Treatment | Year: 2015

Abstract: In this study, the ability of grape stalk residue of wine production to remove Cd(II) from aqueous solution by adsorption has been investigated through batch experiments. The grape stalk was characterized in detail by Fourier transform infrared spectrophotometer, scanning electron microscopy, BET, particle size, and elemental analysis. The Cd(II) adsorption property of the grape stalk was evaluated as a function of pH, adsorbent dosage, particle size, initial Cd(II) concentration, and temperature. The adsorption kinetics was evaluated with the pseudo-first-order, second-order kinetics models, and intraparticle diffusion model. The equilibrium data were analyzed using Langmuir and Freundlich isotherms. The adsorption kinetics followed the second-order rate law. The adsorption of Cd(II) onto the grape stalk is fitted to the Langmuir isotherm, and the maximum adsorption capacity was 21.5 mg-Cd(II)/g. Thermodynamic parameters including the changes of enthalpy (ΔH), free energy (ΔG), and entropy (ΔS) were evaluated. The calculated thermodynamic parameters showed that adsorption of Cd(II) was spontaneous and exothermic under examined conditions. © 2014 Balaban Desalination Publications. All rights reserved.

Yerlikaya E.,Siirt State University | Erdogan O.,Ataturk University | Demirdag R.,Agr Ibrahim Cecen University | Senturk M.,Agr Ibrahim Cecen University | Kufrevioglu O.I.,Ataturk University
Turkish Journal of Biochemistry | Year: 2015

Objective: In this study, investigating the effects of inhibition of the enzyme activity of some antitumor drugs and the Cancer-Related Human Carbonic Anhydrase IX (hCA IX) isoenzyme expressing as a SUMO fusion protein in an Escherichia coli expression system were aimed. Methods: hCA IX isoenzyme was expressed using SUMO fusion technology. The fusion protein was expressed ina totally soluble form and the expression was verified by SDS-PAGE analysis. Affinity chromatography was used in the purification processes. The effects of certain antitumor drugs on enzyme activity were investigated in vitro conditions by using esterase activity. IC50 values of drugs showing the inhibitory effect were calculated. Inhibition types and Ki values for antitumor drugs, which inhibit the enzyme, were determined by separately plotting Lineweaver Burk plots. Results: The molecular weight of the fusion protein was approximately 85kDa. The optimal induction concentration of IPTG and the growth temperature were found to be1.0mM and 30oC. The fusion protein was purified at approximately 3.07-fold with a yield of 92.58%, and a specific activity of 43707EU/mg proteins by nickel nitrilo-triacetic acid resin chromatography. Conclusion: Our work is extremely important because CA IX plays a clinical role as a biomarker in cancer diagnosis and the use of specific inhibitors of the CA IX enzyme will be useful in the fight against cancer. In vitro inhibition studies on the recombinant hCA IX enzyme can shed light on the development of anticancer drugs for cancers overexpressing CA IX. © 2015. Turkish Biochemistry Society. All rights reserved.

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