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Kwon B.-E.,Kangwon National University | Song J.-H.,Kangwon National University | Song H.-H.,Agency for Korea National Food Cluster AnFC | Kang J.W.,Yonsei University | And 10 more authors.
PLoS ONE | Year: 2016

The flavonoids mosloflavone, oroxylin A, and norwogonin, which were purified from Scutellaria baicalensis Georgi, significantly protected Vero cells against Coxsackievirus B3 (CVB3)-induced cell death. To investigate the in vivo antiviral activity of oroxylin A, we intraperitoneally inoculated CVB3 into 4-week-old BALB/c mice. Body weights and blood glucose levels of the mice were decreased after CVB3 infection, and these changes were attenuated by the administration of oroxylin A. Importantly, treatment of mice with oroxylin A reduced viral titers in the pancreas and decreased the serum levels of the inflammatory cytokines including interleukin-6 (IL-6) and tumor necrosis factor (TNF)-α. Additionally, the administration of oroxylin A mitigated the histological pancreatic lesions and apoptotic cell death induced by CVB3 infection and increased the levels of phospho-eIF2α in infected pancreata. The results suggest that oroxylin A may represent a potent antiviral agent against CVB3 infection. © 2016 Kwon et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Ryu H.W.,Korea Research Institute of Bioscience and Biotechnology | Song H.-H.,Agency for Korea National Food Cluster AnFC | Kim K.O.,Korea Research Institute of Bioscience and Biotechnology | Park Y.J.,Korea Research Institute of Bioscience and Biotechnology | And 3 more authors.
Industrial Crops and Products | Year: 2016

The common essential environmental factors for plant growth are play the regulatory and signaling role in the developmental and metabolic processes of plants. A study aimed at determining the metabolite changes in wild and cultivated Euphorbia supina and their effects on its biological activity was conducted. These two plant groups were clearly discriminated from one another on principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) score plots. More detailed analyses of the extracts by ultra-performance liquid chromatography (UPLC) coupled to high-resolution electrospray ionization mass spectrometry (HRESIMS) on a quadrupole time-of-flight (QTof) analyzer was revealed approximately twenty phytochemicals, including gallotannins, ellagitannins and glycosidic derivatives of flavonoids the secondary phytochemicals in wild E. supina. The accumulation of these metabolites was responsible for the antioxidant content and activity in the group of plants, highlighting their potential utility in understanding the effects of environmental stress conditions on crop productivity and improving the bioactivity of medicinal plants. © 2016.

Song H.-H.,Agency for Korea National Food Cluster AnFC | Ryu H.W.,Korea Research Institute of Bioscience and Biotechnology | Kim H.-S.,Korea Research Institute of Bioscience and Biotechnology | Kim C.-S.,Korea forest Research Institute | And 3 more authors.
Journal of Functional Foods | Year: 2016

The metabolite changes in Korean mistletoe, as functional food, and their effects on its biological activity were monitored. The Korean mistletoe species were successfully distinguished by hierarchical clustering analysis (HCA) in atmospheric-pressure chemical ionization (APCI) and electrospray ionization (ESI) mode. As shown by the PCA scatter plot and loading plot, the Santalaceae family exhibited significant markers derived from oleanolic acid, whereas the Loranthaceae family exhibited tentative markers derived from glycosidic derivatives of quercetin and kaempferol. The mistletoe extracts also inhibited plasminogen activator inhibitor 1 (PAI-1) differently depending on the mistletoe family. The Santalaceae family was shown to be a potent inhibitor of PAI-1, with an IC50 value of around 40.0 μg/mL. In contrast, the Loranthaceae family extracts did not inhibit PAI-1. Importantly, discrimination was shown across the chemotaxonomy of the plant, which correlated strongly with the oleanolic acid contents in Korean mistletoe. © 2016 Elsevier Ltd.

Ryu H.W.,Korea Research Institute of Bioscience and Biotechnology | Song H.-H.,Agency for Korea National Food Cluster AnFC | Shin I.-S.,Chonnam National University | Cho B.O.,Ato Q and A corporation | And 4 more authors.
Journal of Functional Foods | Year: 2015

Resveratrol oligomers from the extract of Peaonia lactiflora seeds were investigated for the first time and their potential role as nutraceuticals to treat airway inflammation based on their anti-inflammatory activity examined. After the isolation of five major peaks (suffruticosol B, suffruticosol A, trans-ε-viniferin, trans-gnetin H, and cis-suffruticosol D) of a P. lactiflora extract using a one-step high-speed counter current chromatograhy (HSCCC) process, the anti-inflammation activity of each compound was evaluated in the LPS-stimulated RAW264.7 macrophages. Suffruticosol A showed potent levels of inhibition in the expression/production of NO, iNOS, and pro-inflammatory cytokines (TNF-α, IL-6, and IL-1β) in the assay. Additionally, suffruticosol A exhibited a significant suppression of the increased inflammatory cell count, protein content, pro-inflammatory cytokines and the production of reactive oxygen species in the bronchoalveolar lavage fluid of acute airway inflammation murine model, in which cigarette smoke and LPS exposure were both used as pro-inflammatory stimuli. These experimental results indicate that suffruticosol A has significant effects both in vitro and in vivo to be a potential candidate for airway inflammation therapeutics based on its anti-inflammatory activity. © 2015 Elsevier Ltd.

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