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Santos-Cividanes T.M.,Agencia Paulista de Tecnologia dos Agronegocios | dos Anjos A.C.R.,Agro Energia Santa Luzia Ltda | Cividanes F.J.,Sao Paulo State University | Dias P.C.,Sao Paulo State University
Neotropical Entomology | Year: 2011

The lady beetle Coleomegilla maculata (De Geer) is a natural enemy of several insect pests and feeds on pollen and nectar to survive periods when prey is scarce. The effect of the feeding interval on the development, survival, fecundity, and longevity of C. maculata was determined. Newly hatched larvae of C. maculata were reared individually and fed with eggs of the Mediterranean flour moth Anagasta kuehniella (Zeller) at intervals of one, two, and three days under controlled conditions (23 ± 1°C; 60 ± 10% RH; 12 h phtophase). The duration of larval instars and the total larval stage was prolonged as the feeding interval increased. The larval period lasted on average 9.2 ± 0.19 days when the larvae were fed daily with prey, and 14.6 ± 0.48 days when food was offered at three-day intervals. There was an inverse relationship between food intervals, survival, and weight of larvae and adults of the coccinellid. Survival rate of larvae fed daily was 76.8%, while the rate was 50.0% and 23.4% for larvae fed every two and three days, respectively. Coleomegilla maculata showed fecundity of 781.1 ± 149.02, 563.4 ± 80.81 and 109.0 ± 103.0 eggs when fed daily and at intervals of two and three days, respectively. © 2011 Sociedade Entomológica do Brasil.

Nazareno A.G.,Federal University of Santa Catarina | Zucchi M.I.,Agencia Paulista de Tecnologia dos Agronegocios | dos Reis M.S.,Federal University of Santa Catarina
American Journal of Botany | Year: 2011

Premise of the study: Microsatellite markers were developed for the vulnerable palm species Butia eriospatha (Mart. ex Drude) Becc. to investigate genetic diversity, spatial genetic structure, mating system, and population dynamics. Methods and Results: From a genomic library enriched for GA/CA repeats, 14 sets of primers were isolated and characterized for 50 B. eriospatha samples from two populations. The number of alleles per locus ranged from 2 to 6 (with amplified dinucleotide repeat-based primers); the observed and expected heterozygosities ranged from 0.000 to 1.000 and from 0.120 to 0.690, respectively. At least 86% of primers were also amplified for Butia catarinensis Noblick & Lorenzi, another threatened palm species from the Atlantic Rainforest in Brazil. Conclusions: The new marker set described here will be useful for studies of population genetics of B. eriospatha, and they have been shown to be applicable for other species from the Butia genus. © 2011 Botanical Society of America.

Teixeira E.W.,Agencia Paulista de Tecnologia dos Agronegocios | Message D.,Federal University of Vicosa | Negri G.,University of Sao Paulo | Salatino A.,University of Sao Paulo | Stringheta P.C.,Federal University of Vicosa
Evidence-based Complementary and Alternative Medicine | Year: 2010

Total phenolic contents, antioxidant activity and chemical composition of propolis samples from three localities of Minas Gerais state (southeast Brazil) were determined. Total phenolic contents were determined by the FolinCiocalteau method, antioxidant activity was evaluated by DPPH, using BHT as reference, and chemical composition was analyzed by GC/MS. Propolis from Itapecerica and Paula Cndido municipalities were found to have high phenolic contents and pronounced antioxidant activity. From these extracts, 40 substances were identified, among them were simple phenylpropanoids, prenylated phenylpropanoids, sesqui- and diterpenoids. Quantitatively, the main constituent of both samples was allyl-3-prenylcinnamic acid. A sample from Virginpolis municipality had no detectable phenolic substances and contained mainly triterpenoids, the main constituents being α- and β-amyrins. Methanolic extracts from Itapecerica and Paula Cndido exhibited pronounced scavenging activity towards DPPH, indistinguishable from BHT activity. However, extracts from Virginpolis sample exhibited no antioxidant activity. Total phenolic substances, GC/MS analyses and antioxidant activity of samples from Itapecerica collected monthly over a period of 1 year revealed considerable variation. No correlation was observed between antioxidant activity and either total phenolic contents or contents of artepillin C and other phenolic substances, as assayed by CG/MS analysis. © 2008 The Author(s).

Gouvea L.R.L.,Instituto Agronomico Of Campinas | Silva G.A.P.,Instituto Agronomico Of Campinas | Scaloppi Junior E.J.,Agencia Paulista de Tecnologia dos Agronegocios | Goncalves P.S.,Instituto Agronomico Of Campinas
Pesquisa Agropecuaria Brasileira | Year: 2011

The objective of this work was to assess, during six years, the temporal stability of natural rubber yield of 25 superior Hevea brasiliensis genotypes, using the Wricke, Eberhart & Russell, Lin & Binns, additive main effect and multiplicative interaction (AMMI) analysis, and harmonic mean of the relative performance of the genetic values (HMRPGV) methods. The IAC 40 and IAC 300 genotypes were identified as stable and high yielding by the Eberhart & Russell, Lin & Binns, HMRPGV, and AMMI Biplot methods. The ranking of the other more stable genotypes identified by these analyses was altered. The observed results in the AMMI Biplot agreed with those observed in the Wricke method for identifying stable, but lower yielding genotypes. The simultaneous use of different methods allows a more accurate indication of stable genotypes. Stability analyses based on different principles show agreement in indicating stable genotypes.

Cividanes F.J.,Sao Paulo State University | dos Santos-Cividanes T.M.,Agencia Paulista de Tecnologia dos Agronegocios
Pesquisa Agropecuaria Brasileira | Year: 2010

The objective of this work was to determine the flight patterns and the influence of meteorological factors and Brassicaceae on the populations of the alate aphids Brevicoryne brassicae, Lipaphis erysimi and Myzus persicae. The alate aphids were sampled using yellow water traps between July of 1997 and August of 2005. The Pearson partial correlation was used to determine the influence of air temperature, relative humidity, rainfall and insolation on the abundance of alate. The influence of Brassicaceae was assessed by accumulated degree-days above the temperature threshold of these plants. Lipaphis erysimi was more abundant than M. persicae and B. brassicae. The alate B. brassicae showed the highest flight frequency between August and October, with the abundance peak in September. The flight frequency of L. erysimi and M. persicae were longer than B. brassicae, with the alate abundance peaks of L. erysimi and M. persicae observed from April to November and from June to October, respectively. The population of B. brassicae showed significant correlation with the maximum and minimum temperatures, insolation and relative humidity, while L. erysimi and M. persicae were only affected by insolation and relative humidity.

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