Agencia de Gestion Agraria y Pesquera de Andalucia AGAPA

Sevilla, Spain

Agencia de Gestion Agraria y Pesquera de Andalucia AGAPA

Sevilla, Spain
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Garcia-Bocanegra I.,University of Cordoba, Spain | Arenas-Montes A.,University of Cordoba, Spain | Napp S.,Autonomous University of Barcelona | Jaen-Tellez J.A.,Consejeria de Agricultura y Pesca de la Junta de Andalucia | And 3 more authors.
Veterinary Microbiology | Year: 2012

West Nile virus (WNV) is recognized as an emerging zoonotic pathogen, whose incidence in horses, humans and birds has increased significantly in different European countries in the last decade. A serosurvey study was carried out in non vaccinated horses to determine the geographical distribution of WNV in Andalusia (Southern Spain), and to assess the factors that influence the risk of WNV infection in horses. Antibodies to WNV were detected in 54 out of 510 horses analyzed by a blocking ELISA, of which 36 were confirmed by micro virus neutralization test (7.1%; CI95%: 4.9-9.3). A total of 28 out of the 348 equine herds (8.3%; CI95%: 5.4-11.2) had at least one seropositive animal. A generalized estimating equations model showed that the main risk factors associated to WNV seroprevalence were: number of horses within the holding (low), transport of the horse within the last six months (Yes) and presence of mosquitoes in the holding (Yes). The results demonstrated that WNV circulation in Andalusia was more widespread than previously reported. Besides, the distribution of WNV infections was not homogeneous as significant differences among provinces were observed. The results show the need to improve the active surveillance in Spain, so that the early detection of WNV circulation allows the establishment control measures such as vaccination and implementation of vector control programs during the risk period. © 2012 Elsevier B.V.


Garcia-Bocanegra I.,University of Cordoba, Spain | Allepuz A.,Autonomous University of Barcelona | Perez J.J.,Laboratorio Of Sanidad Y Produccion Animal | Alba A.,Autonomous University of Barcelona | And 6 more authors.
Veterinary Journal | Year: 2014

Six serological assays for the diagnosis of ovine brucellosis, due to Brucella melitensis were evaluated. Reference serum samples from sheep of known B. melitensis infection status (n= 118) were assessed using the Rose Bengal test (RBT), complement fixation test (CFT) and four commercial enzyme-linked immunosorbent assays (ELISAs), including two indirect ELISAs (iELISAs), one competitive ELISA (cELISA) and one blocking ELISA (bELISA).The highest differential positive rates (DPR) were obtained with the cELISA and bELISA, while the lowest DPR was estimated using iELISAs. A latent class analysis was performed to estimate the accuracy of the CFT, RBT and bELISA using 1827 sera from sheep undergoing testing as part of a surveillance and control programme. Lower sensitivity and specificity were obtained for the three serological tests when the field samples were used. A higher DPR was achieved by the CFT, compared to bELISA and RBT. The results suggest that ELISAs, and particularly the bELISA, might be suitable for inclusion in the European Union legislation on intra-community trade for diagnosing B. melitensis infection in sheep, as it has a similar test performance compared to the RBT. © 2013 Elsevier Ltd.


Gonzalez-Dugo M.P.,IFAPA Centro del Llano | Escuin S.,Agencia de Gestion Agraria y Pesquera de Andalucia AGAPA | Cano F.,Agencia de Gestion Agraria y Pesquera de Andalucia AGAPA | Cifuentes V.,Confederacion Hidrografica del Guadalquivir | And 5 more authors.
Agricultural Water Management | Year: 2013

Water management at different decision levels may be supported by the assessment of evapotranspiration (ET) on large spatial scales. In this study, a previously validated approach to estimating unstressed ET, based on the ability of vegetation indices to trace crop growth and thus to derive basal crop coefficients, has been applied to the irrigated areas of the Guadalquivir river basin in southern Spain. Vegetation indices, provided by a series of high spatial resolution satellite images for 2007, 2008 and 2009, supported the assessment of daily to seasonal ET of individual fields, enabling crop-oriented and individual water use to be analysed. The segmentation of the basin into zones with homogeneous climate and crop-growth patterns was the first step towards crop identification based on temporal trends in the Soil-Adjusted Vegetation Index (SAVI). Non-permanent crops were classified with good accuracy. Existing spatial databases of permanent crops enabled land use to be determined. The applied methodology has been compiled in a planning and operational tool named MINARET (MonitorINg irrigated AgricultuRe ET) for routinely monitoring crop water consumption in the irrigated lands of the Guadalquivir basin and it is now available for the Guadalquivir river authority. © 2013 Elsevier B.V. All rights reserved.

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