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Nový Jičín, Czech Republic

Dedkova K.,VSB - Technical University of Ostrava | Janikova B.,VSB - Technical University of Ostrava | Matejova K.,AGEL Laboratory | Cabanova K.,VSB - Technical University of Ostrava | And 4 more authors.
Journal of Photochemistry and Photobiology B: Biology | Year: 2015

The paper reports laboratory preparation, characterization and in vitro evaluation of antibacterial activity of ZnO/graphite nanocomposites. Zinc chloride and sodium carbonate served as precursors for synthesis of zinc oxide, while micromilled and natural graphite were used as the matrix for ZnO nanoparticles anchoring. During the reaction of ZnCl2 with saturated aqueous solution of Na2CO3a new compound is created. During the calcination at the temperature of 500 °C this new precursors decomposes and ZnO nanoparticles are formed. Composites ZnO/graphite with 50 wt.% of ZnO particles were prepared. X-ray powder diffraction and Raman microspectroscopy served as phase-analytical methods. Scanning electron microscopy technique was used for morphology characterization of the prepared samples and EDS mapping for visualization of elemental distribution. A developed modification of the standard microdilution test was used for in vitro evaluation of daylight induced antibacterial activity and antibacterial activity at dark conditions. Common human pathogens served as microorganism for antibacterial assay. Antibacterial activity of ZnO/graphite composites could be based on photocatalytic reaction; however there is a role of Zn2+ ions on the resulting antibacterial activity which proved the experiments in dark condition. There is synergistic effect between Zn2+ caused and reactive oxygen species caused antibacterial activity. © 2015 Published by Elsevier Ltd.

Dedkova K.,VSB - Technical University of Ostrava | Janikova B.,VSB - Technical University of Ostrava | Matejova K.,AGEL Laboratory | Peikertova P.,VSB - Technical University of Ostrava | And 3 more authors.
Journal of Photochemistry and Photobiology B: Biology | Year: 2015

This paper describes laboratory preparation, characterization and antibacterial activity testing of ZnO/kaoline composites. ZnO/kaoline composites with 50 wt.% of ZnO were laboratory prepared, dried at 105 °C and calcined at 500 °C. XRPD analysis revealed that thermal treatment caused the phase transformation of Zn containing precursor into ZnO. Scanning and transmission electron microscopy techniques were used for characterization of morphology of the prepared samples. A standard microdilution test was used for evaluation of antibacterial activity using four common human pathogens (Staphylococcus aureus, Escherichia coli, Enterococcus faecalis and Pseudomonas aeruginosa). Daylight was used for induction photocatalytically based antibacterial activity. Second possible explanation of antibacterial activity of ZnO/kaoline could be the presence of biologically available forms of zinc. During the antibacterial activity assays the ZnO/kaoline composites exhibited antibacterial activity, where differences in an onset of the antibacterial activity and activity against bacterial strains were observed. The highest antibacterial activity was observed against S. aureus, where the lowest value of minimum inhibitory concentration was determined equal to 0.41 mg/ml. © 2015 Elsevier B.V. All rights reserved.

Soumarova R.,Comprehensive Cancer Center | Soumarova R.,University of Ostrava | Soumarova R.,AGEL Research and Training Institute Novy Jicin Branch | Boday A.,AGEL Laboratory | And 12 more authors.
Neoplasma | Year: 2015

Prostate specific antigen and digital rectal examination have low specificity for detecting prostate cancer and they poorly predict the presence of aggressive disease. We present recent findings on PCA3 and TMPRSS:ERG fusion and assessed the relationship between PSA, urine PCA3 and TMPRSS2:ERG and corelation with pathological findings. We tested the PCA3 score in two groups. The first comprised 96 men treated in urology out-patient units with suspicion of prostate cancer, who had elevated PSA and/or positive DRE. The second group comprised 28 patients, who were treated by radiation for localised prostate cancer, and whose PCA3 was regularly monitored. A further cohort comprised patients with already-diagnosed tumors, who had undergone radical prostatectomy. With these, using histopathological samples, we examined samples of the TMPRSS2:ERG fusion gene and compared the results with Gleason score values and level of PSA. We also examined the TMPRSS2:ERG gene in patients who had positive biopsy. Part of the genetical analysis was also an examination of the MSMB gene.The sensitivity of PCA3 testing was 66.7% and the specificity 78.5%. TMPRSS2:ERG gene was correllated with the Gleason score. Neither the TMPRSS2:ERG (p=0.13) nor the MSMB (p=0.556) genotype had an influence on the value of the Gleason score. However a difference was found between the homozygote and wild type (WT) in the TMPRSS2 gene.FISH analysis of TMPRSS/ERG gene fusion was evaluated as positive in 8 (36.8%) of the biopsically verified tumors and in 20 (37.3%) of the evaluated patients after RAPE of parafin slicing.We did not confirm a corellation between fusion and Gleason score (p=0.29).PCA3, with its higher sensitivity in comparison with PSA, is more useful for eventual screening examination. Identification of further molecular markers such as TMPRSS2, may be very promising ways to determine further prognosis of patients with prostate cancer. © 2015 Cancer Research Institute Slovak Acad. of Sciences. All rights reserved.

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