Afzal Research Institute NGO

Kermān, Iran

Afzal Research Institute NGO

Kermān, Iran

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Karamouzian S.,Neuroscience Research Center | Karamouzian S.,Kerman Medical University | Eskandary H.,Neuroscience Research Center | Eskandary H.,Afzal Research Institute NGO | And 6 more authors.
Spine | Year: 2010

STUDY DESIGN.: This study measured the frequency of lumbar intervertebral disc nucleus pulposus microscopic calcification and angiogenesis in adult patients undergoing discectomy compared to normal cadavers. OBJECTIVE.: The results were compared to determine the relationship between disc microscopic calcification with disc degeneration type, histopathological angiogenesis, patients' age, gender, and duration of symptoms. SUMMARY OF BACKGROUND DATA.: True frequency of microscopic calcification in normal or degenerated lumbar discs have not been fully defined nor linked to disc degeneration type and angiogenesis. Some studies demonstrated that angiogenesis and calcification are related to each other in several another tissues. METHODS.: The frequency of microscopic calcification in specimens of disc nucleus pulpous obtained from 2 groups were measured: specimens were obtained during surgery from 90 consecutive patients of 15 to 50 years old suffering from disc herniation in single level of L4-L5 or L5-S1 between 2005 and 2006, 60 additional specimens of lumbar disc nucleus pulposus were obtained from normal cadavers of the same ages. Calcification was determined microscopically by Von Kossa staining and angiogenesis by H/E, and type of degeneration radiologically by Modic classification. RESULTS.: Frequency of microscopic calcification was significantly higher in degenerated disc than normal cadaveric (54.4% vs. 6.7%) and was higher in Modic type III than type I (III: 95.0%, II: 57.4%, I: 13.0%), also prevalence of angiogenesis was significantly higher in patients than cadaveric discs (41.0% vs. 6.7%) and in calcified than noncalcified discs (59.2% vs. 19.5%) (P < 0.001). There was no relationship between disc calcification and patients' gender and level of discectomy. CONCLUSION.: Disc nucleus pulposus microscopic calcification is a common event occurring in adult patients suffering from lumbar disc herniation. Mechanisms that link disc degeneration, angiogenesis, and calcification remain a focus for further researches that may be useful in future medical treatments before surgical treatment of lumbar disc herniation. © 2010, Lippincott Williams & Wilkins.


Karamouzian S.,Neuroscience Research Center | Karamouzian S.,Kerman Medical University | Eskandary H.,Neuroscience Research Center | Eskandary H.,Afzal Research Institute NGO | And 6 more authors.
Spine | Year: 2011

Study Design. An experimental study to measure the depth of penetration of new vessels in degenerated intervertebral disc in rat. Objective. To evaluate the effects of atorvastatin on angiogenesis in experimental disc degeneration in rat. Summary of Background Data. Back pain is strongly associated with degenerated intervertebral disc and management of this condition is still empirical. Decrease of nucleus nutrition due to loss of vascularity with aging may aggravate the process of disc degeneration. So, angiogenesis may be useful in the healing process of degenerated disc. In this study, we wanted to evaluate the effect of atorvastatin, whose stimulating effect on angiogenesis on other tissues was shown in several studies, on degenerated intervertebral disc in rat. Methods. Atorvastatin was administered intraperitoneally for 6 weeks in doses of 1, 4, and 8 mg/kg in rats after experimental disc degeneration. The rats intervertebral disc sections were stained immunohistochemically for von Willebrand Factor to evaluate the depth of vessels penetration and degree of vascularity. Results. In the nonoperated control group, the intervertebral discs were avascular. But experimental disc degeneration promoted angiogenesis. In this group, the mean of penetration was 49.25 μ (standard deviation = 19.905). Atorvastatin stimulated angiogenesis after experimental disc degeneration in the rats and the angiogenesis was more significant in the high and medium dose groups than operated control group. High-dose atorvastatin could not inhibit angiogenesis in experimental degenerated disc. There was no any significant difference in degree of vascularity among the groups. Conclusion. Atorvastatin stimulates angiogenesis in experimental disc degeneration in rats. But, it does not show a biphasic effect. © 2011, Lippincott Williams & Wilkins.


Moghaddam S.D.,Kerman Medical University | Haghdoost A.-A.,Kerman Medical University | Hoseini S.H.,Shiraz University of Medical Sciences | Ramazani R.,Iran Ministry of Health and Medical Education | Rezazadehkermani M.,Afzal Research Institute NGO
Hepatitis Monthly | Year: 2010

Background and Aims: Hepatocellular carcinoma (HCC) is a well-known consequence of chronic liver disease (CLD). The aim of this study was to extract the HCC incidence rate in the province of Kerman, located in southern part of Iran, and compare the data with other parts of the country. Materials and Methods: All medical records related to HCC were collected through hospitals or outpatient services in public or private centers. The records of all oncology, radiotherapy, and pathology centers in Kerman province were actively searched between 1999 and 2006. The annual incidence of HCC around the country was calculated, using the national cancer registry database provided by the Health Ministry of IR Iran from 2005 to 2006. Using Stata version 8, the crude and age-sex-standardized annual incidence rates were computed. Results: The crude annual incidence rates of HCC per 100,000 persons in Kerman and Iran were 0.522 (95% CI = 0.238- 0.88) and 0.199 (95% CI = 0.167-0.234), respectively. When adjusting for age and sex, the annual incidence rates of HCC in Kerman and Iran were 0.7 (95% CI = 0.4-1.1) and 0.2 (95% CI = 0.2-0.3) per 100,000 persons, respectively (P<0.01). The mean age of patients in Kerman was around 5.5 years younger than other parts of Iran (56.17 ± 18.32 years versus 61.68 ± 14.62 years; P=0.004). Conclusions: In general, the incidence of HCC is not very high in Iran; however, the higher incidence of HCC in Kerman and also the lower age of onset mandates further research to detect HCC's risk factors in this part of country.


Ezzatabadipour M.,Kerman Medical University | Azizollahi S.,Tehran University of Medical Sciences | Sarvazad A.,Kerman Medical University | Sarvazad A.,Tehran University of Medical Sciences | And 4 more authors.
Andrologia | Year: 2012

The prevalence of cigarette and alcohol consumption is high among young adult males during the reproductive period. The current study aimed to evaluate the impact of concurrent chronic administration of nicotine and ethanol on the quality of sperm in the rat. Fifty healthy Wistar male rats were randomly divided into five groups (n = 10) and were given the following for a period of 50 days: ethanol (E), nicotine (N), ethanol and nicotine (E/N); the control group (C) and an intact (I) group. Body weight as well as the weight, volume and dimensions of the testes and the weight of the cauda epididymidis and vas deference were measured. The concentration, motility, viability and membrane integrity of sperm were also assessed. There were no significant differences between body weight and all testis parameters including weight, volume and dimensions. The concentration and motility of sperm in the E/N group was significantly reduced compared with the control group (P < 0.01). Nevertheless, only a marginally significant decrease in sperm viability was found in the E/N group compared with the control group. The study indicates that concurrent chronic administration of ethanol and nicotine may disturb male reproductive function. © 2012 Blackwell Verlag GmbH.


Karamouzian S.,Kerman Medical University | Karamouzian S.,Afzal Research Institute NGO | Akhtarshomar S.,Kerman Medical University | Saied A.,Dr Bahonar Hospital | Gholamhoseinian A.,Kerman Medical University
Asian Spine Journal | Year: 2015

Study Design: A retrospective study. Purpose: The aim of this study was to evaluate the effects of delayed hypothermia on spinal cord injuries in rats. In addition, the effect of methylprednisolone on therapeutic window of hypothermia was evaluated. Overview of Literature: Several studies have demonstrated that early hypothermia is the most effective neuroprotective modality. However, delayed hypothermia seems to be more practical for patients with traumatic spinal cord injuries. A combination of hypothermia and other neuroprotective methods, such as using methylprednisolone, may help extend the therapeutic window of hypothermia. Methods: One hundred and twenty male rats were categorized into six groups. The rats in five groups were subjected to spinal cord injury using the weight drop method, followed by treatment, consisting of early hypothermia, late hypothermia, late hypothermia plus methylprednisolone, or methylprednisolone only. Biochemical tests including catalase, malondialdehyde, and superoxide level were evaluated in the injured spinal cord. Behavioral functions of the hind limb were evaluated by Basso-Battle-Bresnaham locomotor rating scale and tail-flick tests. Results: Functional and biochemical evaluation showed both early and late hypothermia had significant neuroprotective effects. The treated groups did not differ significantly from one another in the behavioral tests. Hypothermia had better biochemical results compared to methylprednisolone. Also, methylprednisolone was shown to extend the therapeutic window of delayed hypothermia. Conclusions: Hypothermia showed a significant neuroprotective effect, which can be improved with further studies optimizing the duration of hypothermia and the rewarming period. Moreover, the therapeutic effect of the delayed hypothermia can be extended by methylprednisolone. © 2015 by Korean Society of Spine Surgery.


Karamouzian S.,Kerman Medical University | Karamouzian S.,Afzal Research Institute NGO | Saeed A.,Kerman Medical University | Ashraf-Ganjouei K.,Milad Hospital | And 3 more authors.
Archives of Iranian Medicine | Year: 2010

Car accidents and gun-shot injuries are the most common causes of spinal cord injury. Five percent of those patients who have a complete spinal cord injury will experience improvement, to some extent. The lack of specific data in the literature regarding the outcome of earthquake related spinal cord injury justified us to design this study where we evaluated the neurological outcome and related factors of SCI in the Bam earthquake. We retrospectively evaluated the neurological outcome of patients with spinal cord injury who survived the Bam earthquake. In this study all patients with spinal cord injury that resulted from the Bam earthquake who registered in the Welfare Organization of Kerman were studied. The neurological conditions of patients eighteen months after the earthquake were compared with their neurological conditions at the time of the earthquake. A total of 17 (23.9%) patients with grade A impairment based on ASIA impairment Scale (AIS) at the time of the earthquake had significant neurological recovery. In addition, younger patients had better neurological outcomes. Those who had fracture dislocations had a worse outcome. Spinal cord injuries that are due to earthquakes have a better neurological outcome in comparison with other causes of this injury. The patients' ages and types of spinal fractures were significantly related to the neurological outcome.


Massood E.,Kerman Medical University | Maryam K.,Kerman Medical University | Parvin S.,Kerman Medical University | Mojgan M.,Kerman Medical University | And 2 more authors.
Cryo-Letters | Year: 2013

The aim of this study was to evaluate whether vitrification is an effective cryopreservation method for storage of human umbilical cord mesenchymal cells (HUCMs). HUCMs were vitrified using a two step method. The viability of vitrified HUCMs (v-HUCM) and nonvitrified HUCMs (n-HUCM) was determined by Trypan Blue staining. The expression of several markers was evaluated using flow cytometry. The osteogenic, adipogenic and chondrogenic differentiation potential of v-HUCMs and n-HUCMs was determined. The postwarming viability of HUCMs was 95.54 ± 2.30. The expression of surface antigens (strong positive for CD44, CD90 and CD 105; negative for CD34 and CD45) was similar in the above mentioned groups. The v-HUCM cells retained ability to differentiate into osteoblasts, adipocytes and chondrocytes under appropriate culture conditions. The analysis of these results showed that vitrification is a reliable and effective method for cryopreservation of human umbilical cord mesenchymal cells. © CryoLetters.


Kaviani M.,Kerman Medical University | Ezzatabadipour M.,Kerman Medical University | Nematollahi-Mahani S.N.,Kerman Medical University | Nematollahi-Mahani S.N.,Afzal Research Institute NGO | And 4 more authors.
Cytotherapy | Year: 2014

Background aims: Vitrification as an advanced cryopreservation method is recommended for cell storage toward future applications. The purpose of this report was to appraise whether gametogenic potential of these cells is altered by vitrification. Methods: A two steps method was applied for hUCM cells vitrification. An n-hUCM group of hUCM cells served as control. In order to differentiation of hUCM cells into male germ cells, the cells were induced by retinoic acid, testosterone and testicular-cell-conditioned medium. To evaluate induced hUCM cells toward germ cells, we used immunocytochemistry and karyotyping methods. Results: v-hUCM cells similar to n-hUCM cells formed flat cells after gametogenic induction, and showed protein expression of germ-cell-specific markers DAZL, VASA (DDX4) and SCP3. Karyotyping pattern remained unchanged in the either groups. Conclusions: The analysis of these results demonstrates that vitrification does not alter differentiation potential of hUCMs to male germ like cells. These results may set an in vitro pattern to study germ-cell formation from hUCM cells and also as a potential source of sperms for male infertility. © 2014 International Society for Cellular Therapy.


Karamouzian S.,Kerman Medical University | Karamouzian S.,Afzal Research Institute NGO | Ebrahimi-Nejad A.,Kerman Medical University | Shahsavarani S.,Kerman Medical University | And 3 more authors.
Asian Spine Journal | Year: 2014

Study Design: Prospective study. Purpose: We compared the effects of two methods of epidural steroid injection in patients with recurrent disc herniation. Overview of Literature: To our knowledge, there is no previous report of such a comparison in these patients. Methods: The study was performed with 30 patients with relapsed lumbar disc herniation whose pain was not relieved by conservative remedies. The patients were divided into two groups, each of 15 patients, and entered the study for caudal or transforaminal injections. The degree of pain, ability to stand and walk, and the Prolo function score were evaluated in both groups before the injection and 2 months and 6 months after the injection. Results: The degrees of pain reduction in the caudal injection group in the second and sixth months were 0.6 and 1.63, respectively, and in the transforaminal injection group were 1.33 and 1.56, respectively. The difference between the two methods was not statistically significant. Similarly, no other evaluated criterion showed a significant difference between the methods. Conclusions: In the current study, the caudal and transforaminal steroid injection methods showed similar outcomes in the treatment of relapsed lumbar disc herniation. However, more detailed patient categorizing may help in finding possible subgroups with differences.


Moshrefi M.,Kerman Medical University | Moshrefi M.,Afzal Research Institute NGO | Yari N.,University of Yazd | Nabipour F.,Kerman Medical University | And 3 more authors.
Tissue and Cell | Year: 2015

Nowadays, stem cells have been introduced as an appropriate source of regenerative medicine for treatment of type I diabetes. Human umbilical cord matrix-derived mesenchymal cells (hUCMC) have successfully been differentiated into insulin producing cells. The isolated hUCM cells were characterized by the expression of stem cell surface markers and by differentiation into adipocytes and osteocytes. The hUCMCs were cultured with different concentrations of neural conditional medium (NCM) and were induced to differentiate into insulin producing cells (IPCs). As 60% NCM concentration resulted in higher nestin and PDX1 expression, the cells were first exposed to 60% NCM and were then induced for IPCs differentiation. PDX1 and insulin gene expression was evaluated in the treated cells. Also, the secretion capacity of the IPCs was assessed by glucose challenge test. IPCs were transferred under the rat kidney capsule. Blood glucose level, weight gain and immunohistochemistry assessments were done in the treated animals. hUCMC expressed mesenchymal cell surface markers and successfully differentiated into adipocytes and osteocytes. Higher NCM concentration resulted in higher PDX1 and nestin expression. The IPCs expressed insulin and PDX1. IPCs were detectable under the kidney capsule 2 months after injection. IPCs transplantation resulted in a sharp decline of blood sugar level and less weight loss. Differentiated hUCM cells could alleviate the insulin deprivation in the rat model of type I diabetes. In addition, higher NCM concentration leads to more differentiation into IPCs and more nestin and PDX1 expression. Kidney capsule can serve as a suitable nominee for IPCs transplantation. © 2015 Elsevier Ltd.

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