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Massood E.,Kerman Medical University | Maryam K.,Kerman Medical University | Parvin S.,Kerman Medical University | Mojgan M.,Kerman Medical University | And 2 more authors.

The aim of this study was to evaluate whether vitrification is an effective cryopreservation method for storage of human umbilical cord mesenchymal cells (HUCMs). HUCMs were vitrified using a two step method. The viability of vitrified HUCMs (v-HUCM) and nonvitrified HUCMs (n-HUCM) was determined by Trypan Blue staining. The expression of several markers was evaluated using flow cytometry. The osteogenic, adipogenic and chondrogenic differentiation potential of v-HUCMs and n-HUCMs was determined. The postwarming viability of HUCMs was 95.54 ± 2.30. The expression of surface antigens (strong positive for CD44, CD90 and CD 105; negative for CD34 and CD45) was similar in the above mentioned groups. The v-HUCM cells retained ability to differentiate into osteoblasts, adipocytes and chondrocytes under appropriate culture conditions. The analysis of these results showed that vitrification is a reliable and effective method for cryopreservation of human umbilical cord mesenchymal cells. © CryoLetters. Source

Kaviani M.,Kerman Medical University | Ezzatabadipour M.,Kerman Medical University | Nematollahi-Mahani S.N.,Kerman Medical University | Nematollahi-Mahani S.N.,Afzal Research Institute NGO | And 4 more authors.

Background aims: Vitrification as an advanced cryopreservation method is recommended for cell storage toward future applications. The purpose of this report was to appraise whether gametogenic potential of these cells is altered by vitrification. Methods: A two steps method was applied for hUCM cells vitrification. An n-hUCM group of hUCM cells served as control. In order to differentiation of hUCM cells into male germ cells, the cells were induced by retinoic acid, testosterone and testicular-cell-conditioned medium. To evaluate induced hUCM cells toward germ cells, we used immunocytochemistry and karyotyping methods. Results: v-hUCM cells similar to n-hUCM cells formed flat cells after gametogenic induction, and showed protein expression of germ-cell-specific markers DAZL, VASA (DDX4) and SCP3. Karyotyping pattern remained unchanged in the either groups. Conclusions: The analysis of these results demonstrates that vitrification does not alter differentiation potential of hUCMs to male germ like cells. These results may set an in vitro pattern to study germ-cell formation from hUCM cells and also as a potential source of sperms for male infertility. © 2014 International Society for Cellular Therapy. Source

Karamouzian S.,Kerman Medical University | Karamouzian S.,Afzal Research Institute NGO | Ebrahimi-Nejad A.,Kerman Medical University | Shahsavarani S.,Kerman Medical University | And 3 more authors.
Asian Spine Journal

Study Design: Prospective study. Purpose: We compared the effects of two methods of epidural steroid injection in patients with recurrent disc herniation. Overview of Literature: To our knowledge, there is no previous report of such a comparison in these patients. Methods: The study was performed with 30 patients with relapsed lumbar disc herniation whose pain was not relieved by conservative remedies. The patients were divided into two groups, each of 15 patients, and entered the study for caudal or transforaminal injections. The degree of pain, ability to stand and walk, and the Prolo function score were evaluated in both groups before the injection and 2 months and 6 months after the injection. Results: The degrees of pain reduction in the caudal injection group in the second and sixth months were 0.6 and 1.63, respectively, and in the transforaminal injection group were 1.33 and 1.56, respectively. The difference between the two methods was not statistically significant. Similarly, no other evaluated criterion showed a significant difference between the methods. Conclusions: In the current study, the caudal and transforaminal steroid injection methods showed similar outcomes in the treatment of relapsed lumbar disc herniation. However, more detailed patient categorizing may help in finding possible subgroups with differences. Source

Ezzatabadipour M.,Kerman Medical University | Azizollahi S.,Tehran University of Medical Sciences | Sarvazad A.,Kerman Medical University | Sarvazad A.,Tehran University of Medical Sciences | And 4 more authors.

The prevalence of cigarette and alcohol consumption is high among young adult males during the reproductive period. The current study aimed to evaluate the impact of concurrent chronic administration of nicotine and ethanol on the quality of sperm in the rat. Fifty healthy Wistar male rats were randomly divided into five groups (n = 10) and were given the following for a period of 50 days: ethanol (E), nicotine (N), ethanol and nicotine (E/N); the control group (C) and an intact (I) group. Body weight as well as the weight, volume and dimensions of the testes and the weight of the cauda epididymidis and vas deference were measured. The concentration, motility, viability and membrane integrity of sperm were also assessed. There were no significant differences between body weight and all testis parameters including weight, volume and dimensions. The concentration and motility of sperm in the E/N group was significantly reduced compared with the control group (P < 0.01). Nevertheless, only a marginally significant decrease in sperm viability was found in the E/N group compared with the control group. The study indicates that concurrent chronic administration of ethanol and nicotine may disturb male reproductive function. © 2012 Blackwell Verlag GmbH. Source

Moghaddam S.D.,Kerman Medical University | Haghdoost A.-A.,Kerman Medical University | Hoseini S.H.,Shiraz University of Medical Sciences | Ramazani R.,Deputy for Non Communicable Diseases of CDC | Rezazadehkermani M.,Afzal Research Institute NGO
Hepatitis Monthly

Background and Aims: Hepatocellular carcinoma (HCC) is a well-known consequence of chronic liver disease (CLD). The aim of this study was to extract the HCC incidence rate in the province of Kerman, located in southern part of Iran, and compare the data with other parts of the country. Materials and Methods: All medical records related to HCC were collected through hospitals or outpatient services in public or private centers. The records of all oncology, radiotherapy, and pathology centers in Kerman province were actively searched between 1999 and 2006. The annual incidence of HCC around the country was calculated, using the national cancer registry database provided by the Health Ministry of IR Iran from 2005 to 2006. Using Stata version 8, the crude and age-sex-standardized annual incidence rates were computed. Results: The crude annual incidence rates of HCC per 100,000 persons in Kerman and Iran were 0.522 (95% CI = 0.238- 0.88) and 0.199 (95% CI = 0.167-0.234), respectively. When adjusting for age and sex, the annual incidence rates of HCC in Kerman and Iran were 0.7 (95% CI = 0.4-1.1) and 0.2 (95% CI = 0.2-0.3) per 100,000 persons, respectively (P<0.01). The mean age of patients in Kerman was around 5.5 years younger than other parts of Iran (56.17 ± 18.32 years versus 61.68 ± 14.62 years; P=0.004). Conclusions: In general, the incidence of HCC is not very high in Iran; however, the higher incidence of HCC in Kerman and also the lower age of onset mandates further research to detect HCC's risk factors in this part of country. Source

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