Onyesom I.,Delta State University, Abraka |
Osioma E.,African Research Laboratories |
Okereke P.C.,Delta State University, Abraka
Asian Pacific Journal of Tropical Biomedicine | Year: 2015
Objective: To assess the effects of hot water leaf extract of Nauclea latifolia (N. latifolia) on antioxidant status, lipid peroxidation values and parasite levels in hepatic and brain tissue of experimental mice (BALB/c) infected with Plasmodium berghei (P. berghei) malaria. Methods: Forty nine mice were divided into seven groups (n = 7) and used for the study. Group A (control) were given 0.2 mL/kg phosphate buffer saline; Group B mice were infected with P. berghei and treated with phosphate buffer saline. Groups C and D mice were also infected but treated with 200 and 300 mg/kg body weight of leaf extract respectively. Groups E and F mice were not infected, but received 200 and 300 mg/kg of leaf extract respectively. Group G mice were infected and treated with chloroquine (5 mg/ kg). Liver and brain tissues of mice were prepared for both biochemical assay and microscopic examination. Results: Results showed that P. berghei malaria infection induced oxidative stress in both liver and brain tissues as evidenced by the significant (P < 0.05) decrease in antioxidants: superoxide dismutase, reduced glutathione and catalase. These reductions perhaps caused compromise in membrane integrity as indicated by the significant increase in lipid peroxidation product malondialdhyde. Malaria parasites were also identified in these tissues. However, N. latifolia treatment eliminated the parasites in tissues and protected them from oxidative damage even better than chloroquine treatment did, whose anti-malarial potency also cleared tissue parasites. The measurement of protection by N. latifolia against damage was strengthened by the insignificant micro structural alterations. Conclusions: The bioactive phytochemical(s) in N. latifolia should be structured and the mechanism(s) of its antimalarial tendency should be further investigated. © 2015 Hainan Medical University.
Tonukari N.J.,Delta State University, Abraka |
Tonukari N.J.,African Research Laboratories |
Avwioroko O.J.,African Research Laboratories |
Ehwerhemuepha T.,African Research Laboratories
Scientific Research and Essays | Year: 2010
The exciting discipline of biotechnology has drawn the interests of traditional biologists, biochemists, microbiologists, medical and agricultural scientists into applying mathematical and engineering models to understanding biology. Also, the biopharmaceutical, biochemical and agricultural industries are rapidly drawing from and applying the research results of biotechnology. Still, new industries relying on genomics are springing up daily to challenge the way things have been done. © 2010 Academic Journals.