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Santa Clara, CA, United States

The General Hospital Corporation and Affymetrix | Date: 2015-02-13

Methods for diagnosing and treating IgG4-related disease (IgG4-RD), e.g., based on detecting levels of IgG4 mRNA, preferably using a branched DNA assay.

Affymetrix | Date: 2015-03-23

Methods for multiplex amplification of a plurality of targets of distinct sequence from a complex mixture are disclosed. In one aspect targets are circularized using a single circularization probe that is complementary to two regions in the target that flank a region to be amplified. The targets may hybridize to the circularization probe so that 5 or 3 flaps are generated and methods for removing flaps and circularizing the resulting product are disclosed. In another aspect targets are hybridized to dU probes so that 5 and 3 flaps are generated. The flaps are cleaved using 5 or 3 flap endonucleases or 3 to 5 exonucleases. The target sequences are then ligated to common primers, the dU probes digested and the ligated targets amplified.

The General Hospital Corporation and Affymetrix | Date: 2015-02-27

The present application relates to methods for diagnosing and treating multiple myeloma (MM) and non-Hodgkin lymphoma (NHL), e.g., based on the detection of clonal IgK or IgL-expressing cells.

The present invention provides methods, compositions, and kits for storing and enhancing the activity of polymerases and particularly thermostable polymerases. The methods comprise mixing a thermostable polymerase with at least one zwitterionic or ylide surfactant that has at least one PEO group. In another aspect the polymerase is mixed with a blocker such as PLURONIC or TETRONIC or an amine N-oxide derivative thereof. The thermostable polymerase may be reversibly inactivated by treatment with 2-(Methylsulfonyl)ethyl 4-nitrophenyl carbonate. Compositions and kits for performing the process according to the invention are also provided.

A method for detecting the presence or absence of a target polynucleotide in a sample is described.

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