Affiliated Hospital to Binzhou Medical College

Binzhou, China

Affiliated Hospital to Binzhou Medical College

Binzhou, China

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Zhang K.,Affiliated Hospital to Binzhou Medical College | Yang S.-Y.,Wichita State University | Yang S.,Affiliated Hospital to Binzhou Medical College | Yang S.,Wichita State University | And 4 more authors.
Journal of Biomedical Materials Research - Part A | Year: 2015

This study investigated cellular trafficking and inflammatory markers in orthopedic biomaterial particle-challenged human peripheral blood monocytes (PBMCs) using a murine immunodeficiency (SCID) model. Periprosthetic tissues from aseptic loosening patients were transplanted into muscles of SCID mice. PBMCs from the same patients were stimulated in vitro with Ti-6Al-4V, PMMA, UHMWPE, or Co-Cr particles for 3 days before administered intraperitoneally to the periprosthetic tissue-implanted mice. The xenografts were harvested 2 weeks later for histological and molecular analyses. Significant cell infiltration was obvious in the transplanted tissues from mice transfused with Ti-alloy, PMMA and UHMWPE-provoked PBMCs compared to controls, and UHMWPE-provoked PBMCs group accumulated significantly more cells among all groups. There were ubiquitous TRAP+ stained cells in all xenografts from particle-stimulated PBMCs mice. Immunohistochemical staining indicated that significantly more IL-1β and TNF positive cells occurred in Ti and PMMA groups; while the UHMWPE group resulted in stronger positive MCP-1 and IL-6 stains. Polymerase chain reaction (PCR) confirmed overexpression of both IL-1β and TNF in Ti and PMMA-stimulated groups; and more MIP-1α gene expression developed in the UHMWPE group. Overall, different type of orthopedic materials influenced the trafficking ability of particle-activated PBMCs which may depend on upregulation of various proinflammatory cytokines and chemokines. © 2014 Wiley Periodicals, Inc.


Zhao M.,Affiliated Hospital to Binzhou Medical College | Wang Q.,Affiliated Hospital to Binzhou Medical College | Wang S.,Affiliated Hospital to Binzhou Medical College | Wang F.,Affiliated Hospital to Binzhou Medical College | Shu J.,Affiliated Hospital to Binzhou Medical College
Shanghai kou qiang yi xue = Shanghai journal of stomatology | Year: 2013

PURPOSE: To evaluate the shear bond strength and bonding ability between the new nano-hydroxyapatite composite resin and tooth structure.METHODS: Thirty-six healthy isolated molars were randomly assigned to 3 groups according to the material employed: new nano-hydroxyapatite composite resin, composite resin karisma and glass ionomer cement. After thermal cycling, the data of shear bond strength was recorded, and the type of specimen fracture section was observed under stereo microscope. The data was analyzed statistically by SPSS17.0 software package.RESULTS: The average shear bonding strength of nano-hydroxyapatite composite resin was (11.23±4.6082) N/mm(2), and that of composite resin charisma and glass ionomer cement was (14.49±7.9855) N/mm(2) and(10.08±6.3701) N/mm(2), respectively. No significant difference was found among 3 groups. The bonding fracture mode of each specimen had no significant difference(P>0.05).CONCLUSIONS: As a new dental restorative material, new nano-hydroxyapatite composite resin has superior bonding ability and can be used in clinical therapy. Supported by Natural Science Foundation of Shandong Province (Y2008C159).


Yang S.,Shandong University | Yang S.,Wichita State University | Yang S.,Affiliated Hospital to Binzhou Medical College | Zhang K.,Affiliated Hospital to Binzhou Medical College | And 5 more authors.
Journal of Biomedical Materials Research - Part A | Year: 2015

This study compared the particulate and ion forms of a cobalt-chrome (Co-Cr) alloy on the differentiation/activation of preosteoblasts. Mouse preosteoblasts (MC3T3-E1) were cultured in an osteoblast-induction medium in the presence of particulate and ion forms of a Co-Cr alloy, followed by cell proliferation and cytotoxicity evaluations. The maturation and function of osteoblasts were assessed by alkaline phosphatase (ALP) assay and related gene expressions. Both particulate and ion forms of the metals significantly reduced the proliferation of MC3T3-E1 cells in a dose-dependent manner. Similarly, cells challenged with high concentrations of particles and ions exhibited a marked cytotoxic effect and diminished expression of ALP. Real-time (RT) polymerase chain reaction (PCR) data have suggested that cells with Co-Cr particles dramatically promoted over-expression of monocyte chemo-attractant protein-1 (MCP-1), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6), whereas Co2+ ions treatment predominately up-regulated expressions of receptor activator of nuclear factor kappa-B ligand (RANKL), nuclear factor of activated T-cells cytoplasmic 1 (NFATc1), and down-regulated expression of osteoprotegerin (OPG) and Osterix (Osx). Overall, this study provides evidence that both Co-Cr alloy particles and metal ions interfered with the MC3T3-E1 cells for their growth, maturation, and functions. Further, Co-Cr particles exhibited stronger effects on inflammatory mediators, while metal ions showed more influence on inhibition of osteoblast differentiation and promotion of osteoclastogenesis. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 3564-3571, 2015. © 2015 Wiley Periodicals, Inc.

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