Zhao L.-C.,Hebei University |
Zhao L.-C.,Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical College |
Liang J.,Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical College |
Li W.,Jilin Agricultural University |
And 4 more authors.
Molecules | Year: 2011
In this paper, ultrasound-assisted extraction (UAE) was applied to the extraction of anthraquinones (aloe-emodin, rhein, emodin, chrysophanol and physcion) from Rheum palmatum L. The five anthraquinones were quantified and analyzed by high performance liquid chromatography coupled with UV detection (HPLC-UV). The extraction solvent, extraction temperature and extraction time parameters, the three main factors for UAE, were optimized with response surface methodology (RSM) to obtain the highest extraction efficiency. The optimal conditions were the use of 84% methanol as solvent, an extraction time of 33 min and an extraction temperature of 67 °C. Under these optimal conditions, the experimental values agreed closely with the predicted values. The analysis of varianceindicated a high goodness of model fit and the success of RSM method for optimizing anthraquinones extraction in Rheum palmatum L. © 2011 by the authors; licensee MDPI; Basel; Switzerland.
Li S.,Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical College |
Chen Y.,Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical College |
Lin Z.,Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical College |
Cui W.,Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical College |
And 2 more authors.
Archives of Orthopaedic and Trauma Surgery | Year: 2012
Objectives Controversies exist over which type of graft is best for the reconstruction of the anterior cruciate ligament (ACL). The purpose of this systematic review was to evaluate the effectiveness of ACL reconstruction using either hamstring (HT) autografts or bone-patellar tendonbone (BPTB) autografts. Methods We searched the Cochrane Library, MEDLINE and EMBASE for published randomized controlled trials (RCT) comparing HT autografts with BPTB autografts for ACL reconstruction. Data analyses were performed using Cochrane Collaboration RevMan 5.0. Results Nine RCTs (738 patients) met the inclusion criteria. The combined results of the meta-analysis indicated there was a significantly lower rate of negative Pivot test [relative risk (RR) 0.87, 95 % confidence intervals (CI) 0.79-0.96, P = 0.004], anterior knee pain (RR 0.66, 95 % CI 0.45-0.96, P = 0.03) and of kneeling pain (RR 0.49, 95 % CI 0.27-0.91, P = 0.02) in the HT group than in the BPTB group. Conclusions ACL reconstruction with HT autografts or BPTB autografts achieved similar postoperative effects in terms of restoring knee joint function. HT autografts were inferior to BPTB autografts for restoring knee joint stability, but were associated with fewer postoperative complications. © Springer-Verlag 2012;.
Chen G.,Chongqing Medical University |
Huang J.-B.,Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical College |
Mi J.,Chongqing Medical University |
He Y.-F.,Chongqing Medical University |
And 6 more authors.
Molecular Biology Reports | Year: 2012
Rapid and reliable biomarkers of renal allograft rejection have not been available. This study aimed to investigate biomarkers in renal allograft tissue using pro-teomic analysis. Orthotopic kidney transplantations were performed using Fisher (F344) or Lewis rats as donors and Lewis rats as recipients. Syngenic control group (Group I) constituted F344-to-F344 orthotopic kidney allo-trans-plantations (n = 8); and allogenic group (Group II) consisted of F344-to-Lewis orthotopic kidney allo-transplantations (n = 8). Renal tissues were harvested 7 days after transplantation. Samples were analyzed using 2-D electrophoresis and matrix assisted laser desorption ionization-time of flight mass spectrometry. 6 differentially expressed proteins were identified between allogenic group and syngenic control group. A rat model of acute renal allograft rejection was successfully set up. Differentially expressed proteins in renal allograft tissue of rat were detected using proteomic analysis and might serve as novel diagnostic and therapeutic targets in human. Quantitative proteomics, using MALDL-TOF-MS methodology has the potential to provide a profiling and a deeper understanding of acute renal rejection. © 2011 Springer Science+Business Media B.V.
PubMed | Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical College
Type: Clinical Trial | Journal: The heart surgery forum | Year: 2012
The goal of the study was to evaluate the quality of life of children after the older corrective arterial switch operation (ASO) by means of the Pediatric Quality of Life Inventory (PedsQL), version 4.0.The records of 86 patients who had complete transposition of the great arteries plus a nonrestrictive ventricular septal defect, or a Taussig-Bing anomaly, and severe pulmonary arterial hypertension, and who underwent a corrective ASO at an older age (>6 months) between May 2000 and October 2008 were reviewed retrospectively. Eighty survivors were followed up, and the health-related quality of life of the survivors was evaluated with the PedsQL, version 4.0.There were 6 hospital deaths. The mean (SD) follow-up interval was 3.5 2.3 years, and the mean age at last visit was 7.0 1.2 years. Two late deaths occurred, and 8 patients were lost to follow-up. Patients who underwent a corrective ASO at an older age showed acceptable scores for all scales, and they were all comparable with those of a healthy population.Our data suggest that the quality of life of children who undergo a corrective ASO at an older age (>6 months) is acceptable, compared with that of healthy children in China.