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Li W.,Jilin Agricultural University | Zhao L.-C.,the Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical College | Wang Z.,Jilin Agricultural University | Zheng Y.-N.,Jilin Agricultural University | And 2 more authors.
International Journal of Molecular Sciences | Year: 2012

In the present work, we reported the enzymatic preparation of deapio-platycodin D (dPD) and platycodin D (PD) optimized by response surface methodology (RSM) from Radix Platycodi. During investigation of the hydrolysis of crude platycosides by various glycoside hydrolases, snailase showed a strong ability to transform deapio platycoside E (dPE) and platycoside E (PE) into dPD and PD with 100% conversion. RSM was used to optimize the effects of the reaction temperature (35-45 °C), enzyme load (5-20%), and reaction time (4-24 h) on the conversion process. Validation of the RSM model was verified by the good agreement between the experimental and the predicted values of dPD and PD conversion yield. The optimum preparation conditions were as follows: temperature, 43 °C; enzyme load, 15%; reaction time, 22 h. The biotransformation pathways were dPE→dPD3→dPD and PE→PD3→PD, respectively. The determined method may be highly applicable for the enzymatic preparation of dPD and PD for medicinal purposes and also for commercial use. © 2012 by the authors; licensee MDPI; Basel; Switzerland. Source


Li S.,the Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical College | Huang B.,the Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical College | Chen Y.,the Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical College | Gao H.,the Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical College | And 3 more authors.
International Journal of Surgery | Year: 2013

Background: Controversy exists over the use of hydroxyapatite (HA)-coated femoral stems in primary total hip arthroplasty (THA). We conducted a meta-analysis of randomized controlled trials (RCT) to compare the clinical and radiologic outcomes of primary THA using HA-coated versus non-HA-coated femoral stems. Methods: Databases including MEDLINE, EMBASE and the Cochrane Library were searched to find relevant RCTs comparing HA-coated versus non-HA-coated femoral stems in primary THA. Data analyses were performed using RevMan 5.0 (The Cochrane Collaboration). Results: Seven studies (792 hips) met the inclusion criteria. The pooled weighted mean difference (WMD) for the postoperative Harris hip score was 3.04 (95% CI:-4.47 to 10.54, P=0.43). The cumulative risk ratios (RR) for the presence of endosteal condensation and radioactive lines were 1.02 (95% CI: 0.93 to 1.12, P=0.64) and 1.01 (95% CI: 0.90 to 1.14, P=0.81), respectively. Conclusions: This meta-analysis demonstrates that the use of HA-coated femoral stems in primary THA has no clinical or radiological benefits. © 2013. Source


Li S.,the Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical College | Lin Z.,the Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical College | Chen Y.,the Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical College | Gao H.,the Affiliated Ruikang Hospital of Guangxi Traditional Chinese Medical College | And 2 more authors.
Zhonghua wai ke za zhi [Chinese journal of surgery] | Year: 2013

OBJECTIVE: To evaluate the difference of clinical outcomes and radiological outcomes through meta-analysis on the total hip arthroplasty (THA) between hydroxyapatite(HA) coating and non-HA coating femoral stems.METHODS: We searched the MEDLINE, Embase, Cochrane library and CBM for published randomized controlled trial (RCT) comparing HA coating and non-HA coating femoral stems in primary THA clinical outcomes with Harris hip score and incidence postoperative thigh pain, radiological outcomes with presence of endosteal condensation and radioactive line on the prothesis, heterotopic ossification. Data analysis were performed using RevMan 5.0(the Cochrane Collaboration).RESULTS: Ten studies and 917 hips into our analysis, with 464 hips in HA groups and 453 hips in non-HA groups. The combined results of the meta-analysis indicated there was no statistical differences between the two groups on postoperative Harris hip score(WMD = 3.04, 95%CI:-4.47-10.54, P = 0.43) , there was statistical difference on incidence postoperative thigh pain (RR = 0.56, 95%CI:0.33-0.94, P = 0.03) . There were no significant differences between the two groups on presence of endosteal condensation (RR = 1.01, 95%CI:0.91-1.11, P = 0.91), presence of radioactive line (RR = 0.99, 95%CI:0.88-1.11, P = 0.83) and incidence of heterotopic ossification (RR = 0.97, 95%CI:0.77-1.21, P = 0.77).CONCLUSIONS: There are no clinical and radiological benefits in the use of HA coating femoral stems in Primary THA, there is not enough evidence prove the HA can reduce the incidence postoperative thigh pain. Source

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