Affiliated Hospital Of Yanan University
Affiliated Hospital Of Yanan University
Jia K.-P.,Affiliated Hospital Of Yanan University |
Zhao L.-F.,Affiliated Hospital Of Yanan University |
Feng N.,Affiliated Hospital Of Yanan University |
Ma K.,Affiliated Hospital Of Yanan University |
Li Y.-X.,Affiliated Hospital Of Yanan University
International Journal of Clinical and Experimental Medicine | Year: 2017
Background: Acute lower respiratory tract infection (ALRTI) has been attributed to a major cause of global child mortality. Micronutrient deficiencies have been associated with susceptibility to ALRTI. Vitamin D is an immunomodulatory molecule and its deficiency is more prevalent in ALRTI children. The present hospital based investigation aimed to decipher association of vitamin D with ALRTI in Chinese infants. Methods: Total of 110 infants, diagnosed with ALRTI and their mother enrolled in the present study. Matched healthy infants belonging to similar areas were included as healthy controls. Plasma levels of 25-OH vitamin D in all cases, their mothers and controls were quantified by enzyme linked immunosorbent assay (ELISA). Results: Plasma levels of 25-OH vitamin D was significantly lower in ALRTI cases than healthy controls (P<0.0001). Furthermore, infants with Pneumonia displayed reduce levels of vitamin D3 with reference to healthy controls and no statistical significant difference was noticed between Pneumonic infants and subjects with Bronchiolitis. A positive correlation was detected in plasma levels 25-OH vitamin D in ALRTI cases and their respective mothers (P<0.0001, r=0.57). Conclusion: Lowered plasma level of 25-OH Vitamin D is linked with susceptibility to ALRTI. Positive correlation of vitamin D3 in ALRTI cases and their mothers indicated supplementation of vitamin D to pregnant women may reduce the incidence of ALRTI in Chinese children. © 2017, E-Century Publishing Corporation. All rights reserved.
Wang L.-L.,Affiliated Hospital Of Yanan University |
Feng Y.-Q.,Affiliated Hospital Of Yanan University |
Cheng Y.-H.,First Affiliated Hospital Of Xian Jiaotong University
International Journal of Ophthalmology | Year: 2017
AIM: To investigate the effect of high mobility group protein box-1 (HMGB1) siRNA on proliferation and apoptosis of retinoblastoma (Rb) cells. ● METHODS: The expression of HMGB1 in Rb cells were detected by real-time polymerase chain reaction (RT-PCR) and Western blot. Chemically synthesized HMGB1 siRNA was transfected into Y79 cells. The inhibitory rate was also examined by RT-PCR and Western blot. After HMGB1 siRNA transfection, the cell proliferation was analyzed by MTT, and cell apoptosis was detected by Caspase-3 active detection kit. Cell cycle distribution and apoptosis were detected by flow cytometry. ● RESULTS: The expression of HMGB1 significantly ele-vated in Rb cells (P <0.01). After transfected by siRNA, the HMGB1 protein level of Y79 cells was significantly reduced (P <0.01). After siRNA interference HMGB1, the proportion of proliferating cells reduced, and the proportion of quiescent cells increased (P <0.05). In addition, apoptosis rate of Y79 cells increased from 2.03% to 9.10% after interfering with HMGB1 siRNA (P <0.05). ● CONCLUSION: Specific HMGB1 siRNA can inhibit the expression of HMGB1. The effect may be attributed to inhibit the proliferation and promote cell apoptosis. © 2017, International Journal of Ophthalmology (c/o Editorial Office). All rights reserved.
Wan Z.,Affiliated Hospital Of Yanan University |
Wan Z.,Xi'an Jiaotong University |
Ren K.,Xi'an Jiaotong University |
Wen W.,Xi'an Jiaotong University |
And 7 more authors.
Clinical and Experimental Hypertension | Year: 2017
The mechanism by which high-salt and low-potassium diet contributes to hypertension remains poorly understood. Plasma homocysteine (Hcys) is recognized as a primary mediator of blood pressure (BP) response to some diets. Therefore, the present study tried to investigate whether plasma Hcys and BP could be regulated by salt loading in normotensive salt-sensitive (SS) persons, and further explored whether potassium supplementation could reverse the effect. We enrolled 47 normotensive subjects, aged 29–65 years. The protocol included 7 days on a low-salt diet (3g/day, NaCl), 7 days on a high-salt diet (18g/day), and then a high-salt diet with potassium supplementation (4.5g/day) for 7 days. After high-salt intake, BP was significantly increased and potassium supplementation lowered it in the SS group. Plasma Hcys were higher in SS subjects than in salt-resistant (SR) subjects after salt loading (34.4 ± 17.0 μmol/L versus 19.16 ± 6.4 μmol/L, P < 0.01). Plasma Hcys in SS subjects was increased on a high-salt diet than on a low-salt diet (34.4 ± 17.0 μmol/L versus 16.5 ± 8.3 μmol/L, P < 0.01), but plasma Hcys was ameliorated by potassium supplementation (34.4 ± 17.0 μmol/L versus 20.9 ± 10.4 μmol/L, P < 0.01). In SS subjects, the change of mean arterial blood pressure (MBP) correlated significantly and positively with the alteration of plasma Hcys during low-salt to high-salt intake and high-salt to high-salt with potassium supplementation (r = 0.75, P < 0.001; r = 0.74, P < 0.001, respectively). Our results indicate that Hcys may partly mediate the impact of high-salt intake and potassium supplementation on BP in SS subjects. © 2017 Taylor & Francis
Wang L.,Affiliated Hospital Of Yanan University |
Wang F.-F.,Affiliated Hospital Of Yanan University |
Ma Y.-H.,Affiliated Hospital Of Yanan University |
Zhang J.,Affiliated Hospital Of Yanan University |
And 4 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2017
BACKGROUND: Intramedullary nails and locking plates are widely used for two-part proximal humerus fracture. Which is better for two types of implants in patients remains controversial. OBJECTIVE: To determine the clinical outcome of intramedullary nails versus locking plates for two-part proximal humerus fracture according to Cochrane Meta analysis. METHODS: We searched PubMed, SCI, Embase, the Cochranel Library and CBMdisc, VIP information, Wanfang Database, and CNKI for randomized controlled trials and quasi-randomized controlled trials on intramedullary nails and locking plates for two-part proximal humerus fracture. RevMan 5.2 software was used to analyze operation time, intraoperative blood loss, fracture healing time, postoperative complications (heterotopic ossification, pain, screw penetration, necrosis of humeral head), and Constant Score. RESULTS AND CONCLUSION: Six articles of clinical controlled trials were included with 259 patients. 131 patients received intramedullary nails, and 128 patients received locking plates. Meta-analysis displayed that no significant difference in fracture healing time, heterotopic ossification, pain, necrosis of humeral head and Constant Score was detected between intramedullary nails and locking plates for two-part proximal humerus fracture. Operation time, intraoperative blood loss, and screw penetration were significantly less in the fixation with intramedullary nails than that in locking plates (P < 0.05). These findings suggested that compared with locking plates, intramedullary nails method for two-part proximal humerus fractures could reduce screw penetration. © 2017, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.
Zhang S.-J.,Affiliated Hospital Of Yanan University
Academic Journal of Second Military Medical University | Year: 2015
Objective To explore the clinical and pathological features of cotyledonoid dissecting leiomyoma of the uterus with intravascular growth. Method 1 cases of cotyledonoid dissecting leiomyoma of the uterus with intravascular growth was observed pathological feature ,immunohistochemical stained and reviewed related literature. Results uterine muscle layers 15cmx13cmx10cm mass was detected with medical ultrasound in a 42-year-old woman and clinical diagnosis of uterine sarcoma. The naked eye uterine muscle layers in a haphazard mass and serous membrane outer protrusions like placental villi-like appearance were discovered, which was composed of well-differentiated tumors of smooth muscle cell and the distribution of the plexiform vascular with prominent hydropic degeneration. In myometrium at the same time form of venous blood tube has good smooth muscle hyperplasia, did not see beyond the blood vessels. The patient underwent total hysterectomy and the right Ssalpingectomy, no recurrence was found during the follow-up of 6 mouths. Conclusion cotyledonoid dissecting leiomyoma of the uterus with intravascular growth, also known as cotyledonoid hydropic intravenous leiomyomatosis(CHIL), is a very uncommon type of a benign uterine smooth-muscle tumor and the prognosis is good. The worrying appearance of the gross specimen is often mistaken for malignant or non-uterine lesions that may result in overtreatment. It is important to be aware of this entity to prevent overly aggressive treatment for this benign smooth-muscle neoplasm. © 2015 Second Military Medical University Press. All rights reserved.
Wang X.,Clinical Laboratory Of Affiliated Hospital Of Yanan University |
Ren J.,Second Hospital Of Xian Jiaotong University |
An J.,Second Hospital Of Xian Jiaotong University |
Liu W.,Affiliated Hospital Of Yanan University
Biomedical Research (India) | Year: 2016
Psoriasis is a common chronic, inflammatory and proliferative skin disease that is widely associated with multiple factors under a polygenic background. The expression of hypoxia-inducible factor-1α (HIF-1α), macrophage migration inhibitory factor (MIF), cyclooxygenase-2 (COX-2) and vascular endothelial growth factor (VEGF) is associated with psoriasis; however, there have been no systematic studies analyzing the mRNA and protein levels in psoriasis vulgaris tissues. The aim of the present study was to investigate the mRNA and protein levels of these genes in psoriasis vulgaris tissues and assess their correlation. Tissue samples from 45 cases of psoriasis vulgaris lesions and 45 cases of normal skin were collected. The study used semi-quantitative polymerase chain reaction and western blot analysis to detect the mRNA and protein levels in the psoriatic lesions and normal skin tissue. The mRNA and protein levels of HIF-1α, MIF, COX-2 and VEGF were significantly higher in psoriasis vulgaris tissues compared with those in normal skin tissue, all P-values were <0.05. Additionally, the mRNA and protein levels in the psoriatic lesions were positively correlated with each other. In conclusion, these genes may have important roles in the development of psoriasis. © 2016, Scientific Publishers of India. All rights reserved.
Li R.,Xian Fifth Hospital |
Li R.,Xian No 1 Hospital |
Dai G.-R.,Affiliated Hospital Of Yanan University
Chinese Journal of Tissue Engineering Research | Year: 2015
BACKGROUND: Tumor stem cells have self-renewal, drug resistance and metastasis tumorigenicity, which play an important role in occurrence, development and metastasis of tumors. Currently, there are two methods to identify tumor stem cells, namely, in vitro tumor sphere culture experiments and in vivo mouse tumorigenic experiments. However, there ia a lack of reports regarding clinically enriched gastric cancer cells by chemotherapy. OBJECTIVE: To investigate the enrichment of rat gastric cancer stem cells by cisplatin, and to explore the screening methods for their surface marker proteins. METHODS: BCG-823 gastric cancer model was established in rats, and then rat models were randomized into two groups: rats in experimental groups were subjected to intravenous injection of 0. 1, 0. 2, 0. 25, 0. 3 g/L cisplatin via the tail vein; those in control group were injected with normal saline via the tail vein. After three courses of chemotherapy, gastric stem cells-enriched tissues were collected. Tumor surface proteins were extracted using high-throughput protein microarray and identified by western blot assy. Effects of cisplatin on enrichment of rat gastric cancer stem cells and screening methods for surface marker proteins were compared. RESULTS AND CONCLUSION: Cisplatin at a dose of 0. 3 g/L×200 μL exhibited the best therapeutic effects, and moreover, with the dose increasing, the tolerance became worse and the incidence of adverse reaction became higher. Transplantation tumors were verified by hematoxylin-eosin staining. Western blot test results were similar to the findings of protein microarray method, that is, HLA-DQ, PMP22 and Claudin7 protein expressions increased in gastric tissues, but HLA-DR, CD14, CD16 and CD56 protein expression decreased. These findings suggest that cisplatin can be used to enrich gastric cancer stem cells in rats, and to successfully screen the corresponding surface marker proteins. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.
Mu Y.-J.,Affiliated Hospital Of Yanan University |
Feng Y.-C.,Affiliated Hospital Of Yanan University
World Chinese Journal of Digestology | Year: 2016
Gastrin 17 (G17) is a polypeptide hormone secreted by gastrointestinal G cells, and it binds to cholecystokinin receptor (CCKR) to exert its biological function through signal transduction, stimulating the secretion of gastric acid and the growth of gastrointestinal mucosa. In recent years some studies suggest that G17 promotes cell proliferation and inhibits apoptosis. Since serum G17 can provide some clues to the function of gastric mucosa and the presence of gastric cancer and precancerous disease, it is of great significance in the diagnosis of gastrointestinal diseases. However, serum G17 is affected not only by gastric factors such as the lesion, the degree of atrophy, and Helicobacter pylori (H. pylori) infection, but also by extragastric and drug factors. In this article, we discuss the biological characteristics of G17, factors influencing serum G17 and the relationship between serum G17 and gastrointestinal diseases. © The Author(s) 2016.
Gu Q.-Y.,Second Affiliated Hospital Of Xian Jiaotong University |
Gu Q.-Y.,Affiliated Hospital Of Yanan University |
Zhang J.,Second Affiliated Hospital Of Xian Jiaotong University |
Feng Y.-C.,Affiliated Hospital Of Yanan University |
And 2 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2015
Objective: The present study aims to investigate the relationship between genetic polymorphisms in HTR3A and HTR3E and diarrhea predominant irritable bowel syndrome (D-IBS) in a Chinese population. Methods: We enrolled 500 D-IBS patients and 500 age- and sex-matched healthy control subjects to detect the genotypes in HTR3A and HTR3B gene by using of PCR-RFLP method. Results: There were significant difference between the D-IBS patients and the health control subjects in the distribution of genotype and allele of rs1062613 in HTR3A gene. As regarding rs62625044 in HTR3E gene, we found there was a significant different between the case and the control group in the distribution of GA genotype and A allele in female but not in male. Conclusion: The present study suggested that there are associations of D-IBS risk with genetic polymorphisms in HTR3A and HTR3E. © 2015, Int J Clin Exp Med. All rights reserved.
Liu S.-J.,The Center Hospital Of Xian City |
Zhang F.-P.,Affiliated Hospital Of Yanan University
World Chinese Journal of Digestology | Year: 2014
AIM: To investigate the status of hepatitis B virus infection and hepatitis B vaccination in maintenance hemodialysis patients.METHODS: Two hundred patients who under-went maintenance hemodialysis at our hospital from January 2011 to December 2012 were included as a study group, and 200 healthy subjects were used as controls. The status of hepatitis B virus infection and hepatitis B vaccination was analyzed and compared between the two groups.RESULTS: After excluding those positive for HBsAb alone, the rate of hepatitis B virus infection in the study group was 17.5%, significantly higher than that in the control group (P < 0.05). The detection rates of hepatitis B surface antigen (HBsAg), hepatitis B surface antibody (HBsAb), hepatitis B core antibody (HBcAb) and hepatitis B e antibody (HBeAb) differed significantly between the two groups (P < 0.05), although the detection rate of HBeAg showed no significant difference (P > 0.05). The rate of hepatitis B vaccination did not differ significantly between the study group and control group (29.0% vs 31.0%, P > 0.05). The percentage of patients who did not receive vaccination for economic reasons differed significantly between the two groups (P < 0.05), although the percentages of patients who did not receive vaccination for other reasons did not differ (P < 0.05).CONCLUSION: The majority of patients undergoing maintenance hemodialysis have hepatitis B virus infection. Iatrogenic infection control and hepatitis B vaccination should be strengthened to reduce infection. © 2014 Baishideng Publishing Group Inc. All rights reserved.