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Li S.-L.,First Affiliated Hospital of Xian Medical University | Ye Y.,First Affiliated Hospital of Xian Medical University | Yuan X.-H.,First Affiliated Hospital of Xian Medical University
PLoS ONE | Year: 2017

Background: A number of studies have investigated the effect of perioperative blood transfusion (PBT) for patients after radical prostatectomy (RP), with some reporting conflicting results. A systematic review of the literature and a meta-analysis were conducted to explore the association between PBT (autologous or allogeneic) and biochemical recurrence-free survival (BRFS), overall survival (OS) and cancer-specific survival (CSS) in patients undergoing RP. Methods: The PubMed, Medline, Cochrane Library, and Embase databases were searched for published controlled clinical studies on perioperative allogeneic or autologous blood transfusion (BT) and patient survival after RP. STATA software version 12.0 was used for data analysis. We used hazard ratios (HRs) and 95% confidence intervals (CIs) to test the correlation between BT and patient survival after RP. Results: Data from a total of 26,698 patients in ten published studies were included in the meta-analysis. The meta-analysis results showed that autologous BT was not associated with BRFS (HR: 1.06; 95% CI: 0.96±1.18; Z = 1.17; P = 0.24), OS (HR: 0.86; 95% CI: 0.71±1.04; Z = 1.58; P = 0.11), or CSS (HR: 0.98; 95% CI: 0.49±1.96; Z = 0.05; P = 0.96). Allogeneic BT exhibited a significant association with worse BRFS (HR: 1.09; 95% CI: 1.01±1.16; Z = 2.37; P = 0.02), OS (HR: 1.43; 95% CI: 1.24±1.64; Z = 4.95; P<0.01) and CSS (HR: 1.74; 95% CI: 1.18±2.56; Z = 2.81; P = 0.005). Conclusion: Our data showed an association between allogeneic BT and reduced BRFS, OS and CSS in patients after RP. These findings indicate that perioperative blood conservation strategies are important for decreasing the allogeneic BT rate. Copyright © 2017 Li et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Gao F.,PLA Fourth Military Medical University | Gao F.,First Affiliated Hospital of Xian Medical University | Liu Z.,Shaanxi Normal University | Ren W.,Shaanxi Normal University | Jiang W.,PLA Fourth Military Medical University
Neuropsychiatric Disease and Treatment | Year: 2014

Growing evidence indicates brain inflammation has been involved in the genesis of seizures. However, the direct effect of acute inflammation on neuronal circuits is not well known. Lipopolysaccharide (LPS) has been used extensively to stimulate brain inflammatory responses both in vivo and in vitro. Here, we observed the contribution of inflammation induced by 10 μg/mL LPS to the excitability of neuronal circuits in acute hippocampal slices. When slices were incubated with LPS for 30 minutes, significant increased concentration of tumor necrosis factor α and interleukin 1β were detected by enzyme-linked immunosorbent assay. In electrophysiological recordings, we found that frequency of epileptiform discharges and spikes per burst increased 30 minutes after LPS application. LPS enhanced evoked excitatory postsynaptic currents but did not modify evoked inhibitory postsynaptic currents. In addition, exposure to LPS enhanced the excitability of CA1 pyramidal neurons, as demonstrated by a decrease in rheobase and an increase in action potential frequency elicited by depolarizing current injection. Our observations suggest that acute inflammation induced by LPS facilitates epileptiform activity in vitro and that enhancement of excitatory synaptic transmission and neuronal excitability may contribute to this facilitation. These results may provide new clues for treating seizures associated with brain inflammatory disease. © 2014 Gao et al. This work is published by Dove Medical Press Limited.


PubMed | Yihe Hospital, Shaanxi Normal University and First Affiliated Hospital of Xian Medical University
Type: Journal Article | Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2016

Objective To explore the relationship between signal transducers and activators of transcription 3 (STAT3) and nuclear factor B (NF-B) signaling pathway in papillary thyroid carcinoma. Methods The study enrolled 69 patients with thyroid cancer and 58 control individuals. Real-time fluorescence quantitative PCR, ELISA and immunohistochemistry were separately used to detect mRNA and protein expressions of NF-B and STAT3 in normal thyroid and thyroid carcinoma tissues. Results Compared with normal thyroid tissues, both mRNA and protein expressions of NF-B and STAT3 increased significantly in thyroid carcinoma tissues. Immunohistochemistry showed that the positive rate of STAT3 in all patients with thyroid cancer (89.3%) was significantly higher than that in the patients with normal thyroid tissue (10.2%). Conclusion The function of NF-B pathway mediated by STAT3 is activated in patients with thyroid cancer.


Zhang M.,Xi'an Jiaotong University | Li. W.,First Affiliated Hospital of Xian Medical University | Yu L.,Xi'an Jiaotong University | Wu S.,Xi'an Jiaotong University
PLoS ONE | Year: 2014

Background: Hypoxia-inducible factor-1a (HIF-1α) is overexpressed in many human tumors and their metastases, and is closely associated with a more aggressive tumor phenotype. The aim of the present study was to investigate the effect of resveratrol (RES) on the expression of ischemic-induced HIF-1α and vascular endothelial growth factor (VEGF) in rat liver.Methods: Twenty-four rats were randomized into Sham, ischemia/reperfusion (I/R), and RES preconditioning groups. I/R was induced by portal pedicle clamping for 60 minutes followed by reperfusion for 60 minutes. The rats in RES group underwent the same surgical procedure as I/R group, and received 20 mg/kg resveratrol intravenously 30 min prior to ischemia. Blood and liver tissue samples were collected and subjected to biochemical assays, RT-PCR, and Western blot assays.Results: I/R resulted in a significant (P,0.05) increase in liver HIF-1α and VEGF at both mRNA and protein levels 60 minutes after reperfusion. The mRNA and protein expressions of HIF-1α and VEGF decreased significantly in RES group when compared to I/R group (P,0.05).Conclusion: The inhibiting effect of RES on the expressions of HIF-1α and VEGF induced by I/R in rat liver suggested that HIF-1α/VEGF could be a promising drug target for RES in the development of an effective anticancer therapy for the prevention of hepatic tumor growth and metastasis. © 2014 Zhang et al.


Gao Y.,Xi'an Jiaotong University | Gao F.,First Affiliated Hospital of Xian Medical University | Ma J.-L.,Xi'an Jiaotong University | Sun W.-Z.,Xi'an Jiaotong University | Song L.-P.,Xi'an Jiaotong University
OncoTargets and Therapy | Year: 2014

Lung cancer is the major cause of cancer deaths worldwide due to its late diagnosis and poor outcome. Understanding genomic medicine may widen our vision into the oncogenesis of lung cancer and may open the door to improvements in the clinical management of lung cancer. It is well known that almost half of all genes are regulated by microRNAs (miRNAs). This review focuses on the role of miRNAs in lung cancer and also touches on the value of miRNA-based novel therapies for lung cancers. © 2014 Gao et al. This work is published by Dove Medical Press Limited.


Gao Y.,Xi'an Jiaotong University | Chen K.,Zhejiang Sci-Tech University | Ma J.-L.,Xi'an Jiaotong University | Gao F.,First Affiliated Hospital of Xian Medical University
OncoTargets and Therapy | Year: 2014

With the development of many nanomedicines designed for tumor therapy, the diverse abilities of cerium oxide nanoparticles (CONPs) have encouraged researchers to pursue CONPs as a therapeutic agent to treat cancer. Research data have shown CONPs to be toxic to cancer cells, to inhibit invasion, and to sensitize cancer cells to radiation therapy and chemotherapy. CONPs also display minimal toxicity to normal tissues and provide protection from various forms of reactive oxygen species generation. Differential cytotoxicity is important for anticancer drugs to distinguish effectively between tumor cells and normal cells. The antioxidant capabili- ties of CONPs, which enable cancer therapy protection, have also resulted in the exploration of these particles as a potential anticancer treatment. Taken together, CONPs might be a potential nanomedicine for cancer therapy and this review highlights the current research into CONPs as a novel therapeutic for the treatment of cancer. © 2014 Gao et al.


Wang S.,First Affiliated Hospital Of Xian Medical University | Gong W.,First Affiliated Hospital Of Xian Medical University | Tian Y.,First Affiliated Hospital Of Xian Medical University | Zhou J.,First Affiliated Hospital Of Xian Medical University
Respiratory Care | Year: 2016

BACKGROUND: FEV6 can be used as a convenient alternative to FVC. The aim of this study was to determine an alternative to the fixed cutoff points of FEV1/FVC <0.70 suitable for FEV1/FEV6 in primary care. METHODS: Pulmonary function testing was conducted on volunteers recruited from 4 community centers in Xi’an, China, between July and August 2012. Participants underwent 3 FVC maneuvers. The maneuver with the best FEV1 was retained. FVC, FEV1, and FEV6 were measured by portable spirometer. The receiver operating characteristic curves that corresponded to the optimal combination of sensitivity and specificity for FEV1/FEV6 were determined. A kappa test was used to compare the agreement between FEV1/FVC and FEV1/FEV6. The positive predictive value and negative predictive value were also calculated. RESULTS: A total of 767 volunteers participated in this study, of whom 297 were male and 470 were female. Considering FEV1/FVC <0.70 as the accepted standard for COPD, the area under the curve was 98% (P <.001), and the FEV1/FEV6 cutoff, corresponding to the greatest sum of sensitivity and specificity, was 0.72. For the total population, the FEV1/FEV6 sensitivity, specificity, positive predictive value, and negative predictive value were 96.9, 98.8, 95.8, and 99.2%, respectively. The agreement between the 2 cutoff points was excellent, and the kappa value was 0.954. CONCLUSIONS: FEV1/FEV6 <0.72 can be used in primary care as a valid alternative to FEV1/FVC <0.70 as a fixed cutoff point for the detection of COPD in adults. This study suggests that FEV1/FEV6 is an effective and well validated option that should be used in primary care to detect COPD, which is a rampant problem. © 2016, Daedalus Enterprises. All right reserved.


Xu G.,First Affiliated Hospital of Xian Medical University | Wu T.,First Affiliated Hospital of Xian Medical University | Ge L.,First Affiliated Hospital of Xian Medical University | Li W.,First Affiliated Hospital of Xian Medical University
Oncology Research and Treatment | Year: 2015

Aims: The purpose of this study was to assess the effects of adjuvant interventions for radioiodine in patients with thyroid cancer. Materials and Methods: A systematic search was undertaken on July 9, 2014. RevMan 5 software was used to synthesize data. Results: 13 randomized controlled trials were included. The pooled risk ratio of 0.99 (95% confidence interval (CI): 0.96-1.02, p = 0.58) indicated no significant difference in successful thyroid remnant ablation between recombinant human thyroid-stimulating hormone (rhTSH) and thyroid hormone withdrawal in 7 trials. The percentage of patients who had successful ablation was significantly higher in the oral-lithium group than in the control group (p = 0.017). A computerized decision aid improves informed decision-making in patients with early-stage papillary thyroid cancer (PTC) who are considering adjuvant radioiodine treatment (p < 0.001). Amifostine pretreatment did not prevent parenchymal damage to the major salivary gland function after radioiodine treatment (p = 0.2461). Conclusions: The present systematic review suggests that rhTSH, lithium, and computerized decision aids maybe act as beneficial adjuvant interventions for radioiodine in patients with thyroid cancer; however, amifostine does not exhibit helpful effects in thyroid cancer patients treated with radioiodine. © 2015 S. Karger GmbH, Freiburg.


PubMed | First Affiliated Hospital of Xian Medical University
Type: Journal Article | Journal: Respiratory care | Year: 2016

FEV6 can be used as a convenient alternative to FVC. The aim of this study was to determine an alternative to the fixed cutoff points of FEV1/FVC <0.70 suitable for FEV1/FEV6 in primary care.Pulmonary function testing was conducted on volunteers recruited from 4 community centers in Xian, China, between July and August 2012. Participants underwent 3 FVC maneuvers. The maneuver with the best FEV1 was retained. FVC, FEV1, and FEV6 were measured by portable spirometer. The receiver operating characteristic curves that corresponded to the optimal combination of sensitivity and specificity for FEV1/FEV6 were determined. A kappa test was used to compare the agreement between FEV1/FVC and FEV1/FEV6. The positive predictive value and negative predictive value were also calculated.A total of 767 volunteers participated in this study, of whom 297 were male and 470 were female. Considering FEV1/FVC <0.70 as the accepted standard for COPD, the area under the curve was 98% (P < .001), and the FEV1/FEV6 cutoff, corresponding to the greatest sum of sensitivity and specificity, was 0.72. For the total population, the FEV1/FEV6 sensitivity, specificity, positive predictive value, and negative predictive value were 96.9, 98.8, 95.8, and 99.2%, respectively. The agreement between the 2 cutoff points was excellent, and the kappa value was 0.954.FEV1/FEV6 <0.72 can be used in primary care as a valid alternative to FEV1/FVC <0.70 as a fixed cutoff point for the detection of COPD in adults. This study suggests that FEV1/FEV6 is an effective and well validated option that should be used in primary care to detect COPD, which is a rampant problem.


PubMed | Mayo Medical School and First Affiliated Hospital of Xian Medical University
Type: Journal Article | Journal: Journal of cellular physiology | Year: 2016

Brain derived neurotropic factor (BDNF) is emerging as an important player in airway inflammation, remodeling, and hyperreactivity. Separately, there is increasing evidence that sex hormones contribute to pathophysiology in the lung. BDNF and sex steroid signaling are thought to be intricately linked in the brain. There is currently little information on BDNF and sex steroid interactions in the airway but is relevant to understanding growth factor signaling in the context of asthma in men versus women. In this study, we assessed the effect of sex steroids on BDNF expression and secretion in human airway smooth muscle (ASM). Human ASM was treated with estrogen (E2 ) or testosterone (T, 10nM each) and intracellular BDNF and secreted BDNF measured. E2 and T significantly reduced secretion of BDNF; effects prevented by estrogen and androgen receptor inhibitor, ICI 182,780 (1M), and flutamide (10M), respectively. Interestingly, no significant changes were observed in intracellular BDNF mRNA or protein expression. High affinity BDNF receptor, TrkB, was not altered by E2 or T. E2 (but not T) significantly increased intracellular cyclic AMP levels. Notably, Epac1 and Epac2 expression were significantly reduced by E2 and T. Furthermore, SNARE complex protein SNAP25 was decreased. Overall, these novel data suggest that physiologically relevant concentrations of E2 or T inhibit BDNF secretion in human ASM, suggesting a potential interaction of sex steroids with BDNF in the airway that is different from brain. The relevance of sex steroid-BDNF interactions may lie in their overall contribution to airway diseases such as asthma.

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