Zhao A.-J.,Affiliated Hospital of North China |
Zheng M.-G.,Affiliated Hospital of North China |
Wang G.-C.,Affiliated Hospital of North China |
Hou J.-P.,Affiliated Hospital of North China |
And 4 more authors.
Progress in Biochemistry and Biophysics | Year: 2010
To investigate further the role of ABCE1 in 95-D/NCI-H446 lung carcinoma cells, the protein of ABCE1 was knocked-down using an RNAi approach. Effect of siRNA- expressing vector on ABCE1 and E- Cadherin protein expression was evaluated in 95-D/NCI-H446 lung carcinoma cells by Western blot and FACS analysis. A transwell collagen invasion assay was used to assess differences in the invasive properties of cells expressing ABCE1. Treatment of the 95-D or NCI-H446 cells with an RNAi to ABCE1 led to near 85% knockdown of ABCE1 expression after 48 h. Interestingly, knockdown of ABCE1 expression was accompanied by a significantly higher level in E-cadherin expression. And the expression of E-cadherin in siRNA-95D/H446 was much higher than that in either of the control cell lines by FACS analysis. Moreover, cells treated with the ABCE1 RNAi displayed poor less invasion than the control cell lines too.There were close relationships between ABCE1 and E- Cadherin. It has been suggested that silencing ABCE1 expression increases E-cadherin expression and decreases cell invasion in 95-D / NCI-H446 lung carcinoma cells. Source