Zhu H.,Qingdao University |
Pan X.,Affiliated Hospital of Medical College |
Wang X.,Municipal Hospital |
Han B.,Eighth Peoples Hospital |
Xu L.,Qingdao University
Oncology Reports | Year: 2011
The role of the peroxisome proliferator-activated receptor-γ (PPARγ) in cell differentiation, cell cycle arrest and apoptosis has attracted increasing attention. Troglitazone (TGZ), a thiazolidinedione ligand of PPARγ, has been recently described as possessing antitumoral properties. We studied the effects of TGZ on proliferation, growth arrest and apoptosis in PC-3 prostate carcinoma cells and its interaction with the signaling pathways of the activated EFG receptor (EGFR). We observed that TGZ, in a dose-and timedependent manner, inhibited the growth of PC-3 cells independent of PPARγ. In addition, TGZ induced cell cycle arrest and apoptosis. We demonstrated that cell proliferation was stimulated by EFG in a dose-and time-dependent manner and was inhibited by TGZ. The analysis of the main intracellular signaling pathways downstream of the activated EGFR, PI3K-Akt, ERK1/2 cascades and IκB revealed that TGZ reduced the phosphorylation levels of EGFR and of their downstream inter-mediators which mediate EGF stimulated proliferation. In conclusion, simultaneous targeting of EGFR, PI3K-Akt, ERK1/2 and NF-κB by TGZ could be the molecular mechanism by which TGZ exerts its additive inhibitory effects on PC-3 cell proliferation.
Lei Z.,Affiliated Hospital of Medical College |
Nan S.,Affiliated Hospital of Medical College |
Chuan J.,Affiliated Hospital of Medical College |
Lei X.,Affiliated Hospital of Medical College |
Xing G.-Y.,General Hospital of Chinese Peoples Armed Police Force
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2010
BACKGROUND: Avascular necrosis of the femoral head involves pimelosis changes of bone marrow progenitor cells on the proximal end of the femur, and leads to difficulties in repairing the bone remodeling. It still remains unclear whether bone marrow mesenchymal stem cells (BMSCs) can be induce-differentiated into osteoblast precursor at autologous non-osteonecrosis region using in vitro extracorporeal shock wave therapy, and then transplanted in autologous osteonecrosis region, contributing to bone remodeling in the zone of necrosis. OBJECTIVE: To investigate the effect of extracorporeal shock wave on the BMSC differentiation in the non-necrotic area of steroid-induced osteonecrosis of the femoral head in patients. METHODS: This study took the bone marrow of steroid-induced osteonecrosis of the femoral head patients who donated voluntarily. BMSCs were isolated and cultured in vitro by density gradient centrifugation and adherence method. Primary cultured BMSCs were cultured with serum-free medium for 24 hours, and intervened by extracorporeal shock wave of 5 kV/500 frequency for 10 minutes. No extracorporeal shock wave was given in the blank control group. RESULTS AND CONCLUSION: Cells after passage in the extracorporeal shock wave group entered the peak phase of proliferation early, showing osteogenic differentiation trend. Cells in the blank control group presented multi-directional differentiation into other cell types. Proliferation speed was significantly greater in the extracorporeal shock wave group than in the blank control group at various time points (except at day 1) (P < 0.01). Mean positive rate of alkaline phosphatase was about 90% at day 24 in the extracorporeal shock wave group, and about 50% at day 36 in the blank control group. At day 20, alizarin red staining exhibited that mineralized nodules in the extracorporeal shock wave group was (7.0 ± 1.3) per field, but no obvious mineralized nodules were found, and staining was always negative in the blank control group. Core binding factor α1 mRNA expression in the extracorporeal shock wave group was significantly stronger than the blank control group at various time points (except at day 3) (P < 0.01). Osteocalcin mRNA expression was significantly stronger in the extracorporeal shock wave group than in the blank control group at various time points (except at days 3 and 6) (P < 0.01). Results have suggested that appropriate intensity of extracorporeal shock wave can promote proliferation and induce osteoblastic differentiation of BMSCs at non-necrotic region of patients with steroid-induced osteonecrosis of the femoral head.
Wu Z.,Affiliated Hospital of Medical College |
Guo Y.,Affiliated Hospital of Medical College |
Bu J.,Affiliated Hospital of Medical College |
Guo C.,Affiliated Hospital of Medical College |
And 2 more authors.
Chinese Journal of Infection and Chemotherapy | Year: 2012
Objective To investigate the extent of inflammatory reaction induced by nosocomial gram-negative or gram-positive bloodstream infections through comparing some clinical indicators and levels of inflammatory mediators. Methods Prospective analysis was conducted in patients with positive blood culture obtained 48 h after admission to ICU, in terms of patient gender, age, APACHE II and Sequential organ failure assessment (SOFA) scores, underlying diseases, severity of infection, total parenteral nutrition, receiving operation or surgical procedure, mechanical ventilation, blood purification therapy, mixed bacterial bloodstream infection, length of ICU stay and in hospital mortality. The parameters such as temperature, heart rate, WBC count, neutrophils, lymphocytes, levels of C-reactive protein, calcitonin, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) were also compared between the patients with gram-positive bloodstream infection and those with gram-negative bloodstream infection. Results The incidence of severe sepsis and septic shock was significantly higher in the patients with gram-negative bloodstream infection than in those with gram-positive bloodstream infection (88.9% vs. 73%, P= 0.003). Blood levels of TNF-α (0.97±0.54 vs. 0.75±0.32, P = 0.029), IL-1β (7.03±0.12 vs. 2.21 ± 0.09, P = 0.006) and IL-6 (10.59±2.48 vs. 2.55±0.75, P = 0.005) increased more in the patients with gram-negative bloodstream infection than in the patients in gram-positive bloodstream infection. Conclusions The patients with gram-negative bloodstream infection show more severe clinical condition and inflammatory reaction than the patients with gram-positive bloodstream infection. The pathogenic mechanism and extent of the host inflammatory response should be considered when treating bloodstream infections in clinical practice.
Wang Y.,Qingdao University |
Zhang A.,Qingdao University |
Lu S.,Qingdao Municipal Hospital |
Pan X.,Affiliated Hospital of Medical College |
And 8 more authors.
International Immunopharmacology | Year: 2014
Many studies have shown that LPS mainly activates four signal transduction pathways to induce inflammation, namely the p38, ERK1/2, JNK and IKK/NF-κB pathways. Studies have demonstrated that 5′-AMP-induced hypothermia (AIH) exhibits high anti-inflammatory capabilities. In this study, we explore that how AIH inhibits the inflammatory response. Wistar rats were divided into five groups: a control group, an LPS group, a 5′-AMP pre-treatment group, a 5′-AMP post-treatment group and a 5′-AMP group. For each group, plasma and lung were collected from the rats at 6 h and 12 h after LPS injection. ELISA assays were used to detect plasma levels of CD14, CRP and MCP-1. Inflammatory pathway activation and TLR4 expression were assayed separately by Western blot analysis and immunohistochemistry. Our results showed that rats treated with AIH either before or after an LPS-challenge had a significant decrease in plasma levels of CD14, CRP and TLR4 compared with rats that received LPS only. Western blot analysis showed that AIH inhibited the activation of extracellular signal-regulated kinases (ERK) 1/2, p38, c-Jun N-terminal kinase (JNK) and NF-κB in inflammatory rats. Our study concluded that AIH attenuated LPS-induced inflammation mainly by inhibiting activation on the ERK1/2, p38, JNK and NF-κB signaling pathways. © 2014 Elsevier B.V.
Wang B.,Affiliated Hospital of Medical College |
Wang B.,Peking Union Medical College |
Wang B.,National Research Institute for Family Planning |
Meng D.,Affiliated Hospital of Medical College |
And 13 more authors.
Rheumatology | Year: 2011
Objective. We suspect that genes or loci that contribute to coronary artery disease (CAD) may also play a role in the pathogenesis of gout, since hyperuricaemia leads to gout, and serum uric acid (SUA) levels are potential risk factors for CAD. The single nucleotide polymorphism (SNP) rs1333049 (C/G) on chromosome 9p21 has been implicated in previous studies to be associated with CAD. The aim of this study was to evaluate the relationship between this SNP and gout pathogenesis. Methods. Nine hundred Chinese Han were recruited for this study (461 gout patients and 439 gout-free individuals). The rs1333049 SNP and surrounding sequences were PCR sequenced. Results. There was a clear link between the rs1333049 genotypic and allelic frequencies between gout cases and controls (χ2 = 6.81, df = 2, P = 0.033 by genotype; χ = 6.63, df = 1, P = 0.01 by allele). There was a significantly increased risk of gout in carriers of the CC genotype (odds ratio = 1.43, 95% CI 1.07, 1.91). Conclusion. To the best of our knowledge, our findings are the first to establish an association of rs1333049 with gout in a Chinese Han population. Meanwhile, this SNP is homologous to miR-519 and miR-520. © The Author 2011. Published by Oxford University Press on behalf of the British Society for Rheumatology. All rights reserved.
Wang Y.,Affiliated Hospital of Medical College |
Xu B.,Affiliated Hospital of Medical College |
Dai S.,Affiliated Hospital of Medical College |
Zhang Y.,Affiliated Hospital of Medical College |
And 2 more authors.
American Journal of Obstetrics and Gynecology | Year: 2011
OBJECTIVE: We sought to evaluate a conservative treatment modality, angiographic uterine artery embolization (UAE) followed by immediate curettage, in the treatment of cervical pregnancy. STUDY DESIGN: Sixteen patients with cervical pregnancy were first treated by UAE to control or prevent vaginal bleeding. Curettage of cervical canal was performed immediately after UAE to remove gestational tissue from the cervix. Clinical outcome assessments include vaginal bleeding, serum β-human chorionic gonadotropin level, cervical mass, menstruation, fertility, and hospitalization time. RESULTS: Fifteen patients were successfully treated by UAE followed by immediate curettage. One patient at very early gestational age underwent UAE only. Quick regression of serum human chorionic gonadotropin level and cervical mass, fertility preservation, and a short hospital stay were observed. CONCLUSION: UAE followed by immediate curettage is an efficient conservative treatment for cervical pregnancy. This procedure may become a useful alternative to other conservative approaches. © 2011 Mosby, Inc.