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Sun L.-X.,Affiliated Hospital of Chengde Medical College Chengde 067000 | Li W.-D.,Peking University | Lin Z.-B.,Peking University | Duan X.-S.,Affiliated Hospital of Chengde Medical College Chengde 067000 | And 7 more authors.
Cellular Physiology and Biochemistry | Year: 2014

Background/Aims: This study was conducted to determine the potential of Ganoderma lucidum polysaccharides (Gl-PS) in protection against lung cancer patient plasma-induced suppression of lymphocytes. Lung cancer is a major cause of disease and loss of life in the United States and worldwide. Cancer cells release immunosuppressive mediators, such as PGE2, TGF-β, IL-10, and VEGF, to inhibit the immune response to escape from immune surveillance. Gl-PS has been shown to counteract this immune inhibition in an animal cell culture model, and thus to facilitate tumor control. The present study explored whether or not such an effect could also be demonstrated in human lung cancer patients. Methods: Immunofluorescence, flow cytometry, MTT, immunocytochemistry, and western blot analysis were used to assess lymphocyte activation with PHA. Results: The plasma of lung cancer patients suppressed proliferation, CD69 expression, and perforin and granzyme B production in lymphocytes upon activation by PHA, effects that were partially of fully reversed by Gl-PS. Conclusion: Lung cancer patient plasma-induced suppression of lymphocyte activation by phytohemagglutinin may be antagonized fully or partially by Gl-PS, an observation suggesting the potential of Gl-PS in cancer therapy. Copyright © 2014 S. Karger AG, Basel.


Li L.,Affiliated Hospital Of Chengde Medical College Chengde 067000 | Zhang X.,Affiliated Hospital Of Chengde Medical College Chengde 067000 | Wang P.,Affiliated Hospital Of Chengde Medical College Chengde 067000 | Liu Y.,Affiliated Hospital Of Chengde Medical College Chengde 067000
Shanghai kou qiang yi xue = Shanghai journal of stomatology | Year: 2014

PURPOSE: To study the expression of survivin in ameloblastoma and its clinical significance.METHODS: Immunohistochemical S-P method was used to detect the expression of survivin in clinical significance. Seventy cases of ameloblastoma (AB), 15 cases of malignant AB and 30 cases of normal oral mucosa were included. SPLUS13.0 software package was used for statistical analysis.RESULTS: Survivin was strongly expressed in AB and malignant AB. The positive rate in malignant AB was up to 100%, followed by 82.9% in AB. Survivin was weakly expressed in normal oral mucosa, with a positive rate of 30%. The difference among the 3 groups was significant (P<0.05).CONCLUSIONS: Survivin is overexpressed in AB compared to normal oral mucosa. Survivin is involved in the development and progress of AB, and the ability of invasion and canceration of AB is correlated with the overexpression of survivin.


PubMed | Affiliated Hospital Of Chengde Medical College Chengde 067000
Type: Journal Article | Journal: Shanghai kou qiang yi xue = Shanghai journal of stomatology | Year: 2014

To study the expression of survivin in ameloblastoma and its clinical significance.Immunohistochemical S-P method was used to detect the expression of survivin in clinical significance. Seventy cases of ameloblastoma (AB), 15 cases of malignant AB and 30 cases of normal oral mucosa were included. SPLUS13.0 software package was used for statistical analysis.Survivin was strongly expressed in AB and malignant AB. The positive rate in malignant AB was up to 100%, followed by 82.9% in AB. Survivin was weakly expressed in normal oral mucosa, with a positive rate of 30%. The difference among the 3 groups was significant (P<0.05).Survivin is overexpressed in AB compared to normal oral mucosa. Survivin is involved in the development and progress of AB, and the ability of invasion and canceration of AB is correlated with the overexpression of survivin.

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