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Gou W.-F.,Liaoning Medical University | Zhao S.,Liaoning Medical University | Song L.,Liaoning Medical University | Yang X.-F.,Liaoning Medical University | And 3 more authors.
Chinese Journal of Cancer Prevention and Treatment | Year: 2014

OBJECTIVE: To establish the transgenic mouse model of spontaneous gastric epithelial tumors using JCV T antigen initiated by cytokeratin 19 promoter (K19). METHODS: DNA fragments containing T antigen and K19 promoter were separated from constructant by NdeI and then microinjected into the fertilized eggs of C57BL/6J mice. The positive mice were screened by PCR antigen by targeting JCV T antigen. The organs of transgenic mice were histologically examined with JCV T antigen immunostained. RESULTS: The positive mice for K19-JCV T antigen was successfully established by microinjection.There was no abnormal appearance of brain, liver, kidney, esophagus, prestomach, stomach, small intestine, large intestine, pancreas, lung, heart and spleen in 2-month KT7 mouse. The positivity of JCV T antigen was observed in the nucleus of gastric and bronchial epithelial cells. The 13.3%(2/15) of 16-month KT7 mice showed pulmonary adenoma or adenocarcinoma with T antigen positive in the nuclei of carcinoma cells. CONCLUSIONS: The transgenic tumor model provides a direct evidence for the oncogenic role of JCV T antigen insertion in the epithelial cells, a good animal model to investigate the oncogenic mechanisms of JCV T antigen, screen the anti-tumor reagents and monitor the gene therapy. Source

Xiao L.-J.,Liaoning Medical University | Xiao L.-J.,Chengde Medical College | Zhao S.,Liaoning Medical University | Zhao S.,Chengde Medical College | And 5 more authors.
Oncology Letters | Year: 2013

The understanding of proliferative and apoptotic changes has aided the improvement of the diagnosis, treatment and prevention of gastric cancer. The present study aimed to investigate the clinicopathological and prognostic significance of Ki-67, caspase-3 and p53 in gastric cancer. The expression levels of Ki-67, caspase-3 and p53 were evaluated on tissue microarrays of gastric carcinomas specimens by immunohistochemistry and compared with the clinicopathological parameters and survival time of the patients. It was observed that the elder or male patients with gastric cancer showed p53 overexpression compared with the younger or female patients, respectively (P<0.05). The expression of Ki-67 and p53 was positively associated with tumor-node-metastasis (TNM) staging (P<0.05). There was higher caspase-3 and p53 expression in the intestinal-type compared with the diffuse-type of carcinomas (P<0.05). There was a positive correlation among Ki-67, caspase-3 and p53 expression in gastric cancer (P<0.05). A Kaplan-Meier analysis indicated that there was positive correlation between caspase-3 expression and the adverse prognosis of the patients (P>0.05). Cox's proportional hazards model indicated that the patient age, gender, depth of invasion, lymphatic invasion, lymph node metastasis, TNM staging, Lauren's classification and caspase-3 expression were independent prognostic factors for gastric carcinomas (P<0.05). The data indicated that the expression of Ki-67, caspase-3 and p53 may be involved in the progression or differentiation of gastric carcinoma. This expression may be employed as an indicator of the pathobiological behavior and prognosis of gastric carcinomas. Source

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