Zhang J.,First Affiliated Hospital of Liaoning Medical College
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery | Year: 2011
Exploring the characteristics of arytenoid cartilage movement. Using Pro/ENGINEER (Pro/E) software, the cricoid cartilage, arytenoid cartilage and vocal cords were simulated to the three-dimensional reconstruction, by analyzing the trajectory of arytenoid cartilage in the joint surface from the cricoid cartilage and arytenoid cartilage composition. The 3D animation simulation showed the normal movement patterns of the vocal cords and the characteristics of vocal cords movement in occasion of arytenoid cartilage dislocation vividly. The three-dimensional model has clinical significance for arytenoid cartilage movement disorders.
Tian B.,First Affiliated Hospital of Liaoning Medical College
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine | Year: 2012
The expression status of CD74 in breast cancer stem cells and its clinical implications was evaluated in order to lay a foundation for managing breast cancer. Five hundred and eighty breast cancer specimens were enrolled in the study. The relationship between the CD74 protein and clinicopathological parameters as well as prognosis was subsequently determined. In total, 468 (80.69 %) of the 580 breast cases showed CD74-positive expression. After universal analysis, CD74 was observed to be related to lymph node metastasis and triple-negative breast cancer (P = 0.01 and 0.001). Moreover, CD74 expression has a line correlation with lymph node metastasis and triple-negative breast cancer (P = 0.02 and 0.001). Furthermore, periostin was shown to attain a significantly more distant liver metastasis and worse disease-specific survival than those with none or low-expressed CD74 protein (P = 0.001). In the Cox regression test, CD74 protein was detected as an independent prognostic factor (P = 0.001). CD74 is consistently expressed in triple-negative subgroups of breast cancer and might be a new potential marker for triple-negative breast cancer.
Tian B.,First Affiliated Hospital of Liaoning Medical College |
Zhang Y.,Jinzhou Central Hospital |
Li N.,Jinzhou Central Hospital
Cancer Biotherapy and Radiopharmaceuticals | Year: 2013
Objective: To study the expression and regulatory effects of CD146 protein in colorectal cancer and the correlation between CD146 protein expression and the prognosis of colorectal cancer. Materials and Methods: The CD146 protein level was detected by immunohistochemistry staining. The relationship between CD146 expression and clinicopathological parameters of colorectal cancer was determined. Results: It was observed that 216 (20.00%) of the 1080 cases positively expressed CD146 protein. Univariate analyses indicated that CD146 expression was related to histological grade, Duke's stage, and liver metastasis (p=0.001, 0.001, and 0.001, respectively). Spearman correlation analysis showed that CD146 expression has line correlation to histological grade, Duke's stage, and liver metastasis (p=0.02, 0.01 and 0.001, respectively). After multivariate analysis, Duke's stage and CD146 were related to liver metastasis (p=0.01 and 0.001, respectively). In the Cox regression test, histological grade, Duke's stage, and CD146 were detected as the independent prognostic factors (p=0.045, 0.01, and 0.001, respectively). Conclusions: CD146 protein may be a potential biomarker for the postoperative liver metastasis of colorectal cancer. © Copyright 2013, Mary Ann Liebert, Inc. 2013.
Zhang Y.,First Affiliated Hospital of Liaoning Medical College
International Journal of Ophthalmology | Year: 2011
AIM: To evaluate the effectiveness of pneumatic retinopexy(PR) with cryopexy in the therapy of inferior rhegmatogenous retinal detachment(RRD). METHODS: Twenty-two patients(22 eyes) were included in this study. The RRD diagnosis was confirmed by best-corrected visual acuity (BCVA), slit-lamp microscope, indirect ophthalmoscope and Goldman three-mirror contact lens. All patients had undergone PR with cryopexy, and the post-operative follow-up was 6 to 24 months with an average of 12 months. Post-operative BCVA and final anatomical outcome was analyzed. RESULTS: The reattachment rate of single PR was 77% (17/22 eyes), and 2 eyes required additional intravitreal injection of the gas. The total reattachment rate of PR was 86% (19/22 eyes). Three eyes needed additional scleral buckling. Final retinal reattachment was achieved in all 22 subjects (100%). Postoperative BCVA was significantly improved (P<0.01). CONCLUSION: PR is an effective surgery for inferior RRD.
[Comparative study on osteogenic effect of bone marrow mesenchymal stem cells transfected by adenovirus-bone morphogenetic protein 2-internal ribosome entry site-hypoxia inducible factor 1alpha(mu) and by bone morphogenetic protein 2 single gene].
Zhang J.,First Affiliated Hospital of Liaoning Medical College
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery | Year: 2012
To compare the osteogenic effect of bone marrow mesenchymal stem cells (BMSCs) transfected by adenovirus-bone morphogenetic protein 2-internal ribosome entry site-hypoxia inducible factor 1alpha(mu) (Ad-BMP-2-IRES-HIF-1alpha(mu)) and by Ad-cytomegalovirus (CMV)-BMP-2-IRES-human renilla reniformis green fluorescent protein 1 (hrGFP-1) single gene so as to optimize the source of osteoblasts. BMSCs were separated and cultured from 1-month-old New Zealand white rabbit. The BMSCs at passage 3 were transfected by virus. The experiment was divided into 4 groups (groups A, B, C, and D) according to different virus: BMSCs were transfected by Ad-BMP-2-IRES-HIF-1alpha(mu) in group A, by Ad-CMV-BMP-2-IRES-hrGFP-1 in group B, by Ad-CMV-IRES-hrGFP-1 in group C, and BMSCs were not transfected in group D. The optimum multiplicity of infection (MOI) (50, 100, 150, and 200) was calculated and then the cells were transfected by the optimum MOI, respectively. The expression of BMP-2 gene was detected by immunohistochemistry staining after transfected, the expressions of BMP-2 protein and HIF-1alpha protein were detected by Western blot method. The osteogenic differentiation potential was detected by alkaline phosphatase (ALP) activity and Alizarin red staining. The optimum MOI of groups A, B, and C was 200, 150, and 100, respectively. The expression of BMP-2 was positive in groups A and B, and was negative in groups C and D by immunohistochemistry staining; the number of positive cells in group A was more than that in group B (P < 0.05). The expression of BMP-2 protein in groups A and B was significantly higher than that in groups C and D (P < 0.05), group A was higher than group B (P < 0.05). The expression of HIF-1alpha protein in group A was significantly higher than those in the other 3 groups (P < 0.05), no significant difference was found among the other 3 groups (P > 0.05). ALP activity in groups A and B was significantly higher than that in groups C and D (P < 0.05), group A was higher than group B (P < 0.05). Calcium nodules could be seen in groups A and B, but not in groups C and D; the number of calcium nodules in group A was higher than that in group B (P < 0.05). The expression of BMP-2 and osteogenic effect of BMSCs transfected by Ad-BMP-2-IRES-HIF-1alpha(mu) (double genes in single carrier) are higher than those of BMSCs transfected by Ad-CMV-BMP-2-IRES-hrGFP-1 (one gene in single carrier).