Zhang X.-L.,First Affiliated Hospital of Liaoning Medical College |
Zheng S.-L.,First Affiliated Hospital of Liaoning Medical College |
Dong F.-R.,First Affiliated Hospital of Liaoning Medical College |
Wang Z.-M.,Third Affiliated Hospital of Liaoning Medical College
Journal of International Medical Research | Year: 2012
OBJECTIVE: To study the effect of nimodipine on hippocampal regional cerebral blood flow (rCBF) and proinflammatory cytokines in rats with experimental vascular dementia. METHODS: Male Sprague Dawley rats were randomly divided into four groups (n = 15/group): sham operated controls (group A); focal cerebral ischaemia (group B); vascular dementia (group C); and vascular dementia treated with 20 mg/kg nimodipine daily (group D). The Morris water maze test evaluated learning and memory, and magnetic resonance perfusion-weighted imaging was used to measure rCBF. Hippocampal levels of nuclear factor-κB (NF-κB), tumour necrosis factor-α (TNF-α) and interleukin 1b (IL-1β) were measured. RESULTS: Compared with group C, rats in group D demonstrated significantly improved learning ability and significantly increased hippocampal rCBF. The levels of NF-kB, TNF-α and IL-1β were significantly lower in group D than in group C. Hippocampal nerve cell morphology was abnormal in group C but near normal in group D. CONCLUSIONS: Nimodipine improved the symptoms of cognitive impairment, increased rCBF, reduced hippocampal cytokine levels and alleviated neuronal injury in the hippocampus of rats with experimental vascular dementia. © 2012 Field House Publishing LLP.
Zhang Y.,Third Affiliated Hospital of Liaoning Medical College |
Liu L.,First Affiliated Hospital of Liaoning Medical College |
Li C.,First Affiliated Hospital of Liaoning Medical College |
Ai H.,Third Affiliated Hospital of Liaoning Medical College
Cancer Biomarkers | Year: 2014
BACKGROUND: The expressions of leptin and its receptor (ObR) have been observed in human endometrial cancer (EC) cells, and leptin can promote the proliferation of EC cells. However, the correlation between leptin and ObR expressions in EC and the clinicopathology of EC is still unclear. OBJECTIVE: This study investigated the correlation between the expressions of leptin and ObR in EC and the clinicopathology of EC. METHODS: Immunohistochemistry was used in this study. The correlation between the expressions of leptin and ObR in EC and the clinicopathology of EC was analyzed. RESULTS: In the EC specimens, the expressions of leptin and ObR were positively correlated with the invasiveness of the cancer and the obesity of patients, but inversely correlated with histological grade. The percentages of leptin and ObR were significantly higher in the patients with lymph node metastasis. Positive expression of leptin and ObR was associated with poorer prognosis (3-year survival rate). Moreover, the expression of leptin and ObR was associated with positive expression of estrogen receptor. CONCLUSIONS: Leptin and ObR are overexpressed in EC, and their expressions are associated with malignancy, invasion, and metastasis of EC. Thus, leptin and ObR may be important indicators in EC. © 2014 - IOS Press and the authors. All rights reserved.
Li H.,Central Laboratory of Liaoning Medical College |
Song H.,Central Laboratory of Liaoning Medical College |
Luo J.,Central Laboratory of Liaoning Medical College |
Luo J.,First Affiliated Hospital of Liaoning Medical College |
And 3 more authors.
Journal of Experimental and Clinical Cancer Research | Year: 2012
Background: We have reported previously that overexpression of glucose-regulated protein 78 (GRP78) promotes the invasion of hepatocellular carcinoma. However, whether GRP78 knockdown affects the extracellular matrix degradation has not been elucidated. Here we are going to determine whether GRP78 knockdown affect the ECM degradation and the role of MMP-2 and MMP-9 in these process in hepatocellular carcinoma cells. Methods: Human hepatocellular carcinoma cell line SMMC7721 and HepG2 were cultured in DMEM supplemented with 10% FBS, RT-PCR and western blot were used to detect the endogenous expression of GRP78, MMP-2, MMP-9 and TIMP-2 in SMMC7721 and HepG2. GRP78 shRNAs were transfected using lipofection2000. Transwell assay and wound healing assay were used to analyze the invasion of each transfectant. Gelatin zymography and FITC-gelatin degradation assay were employed to investigate the capabilities of ECM degradation of each transfectant. MTT assay was used to determine the proliferation status. Western blot was employed to detect the expression of matrix metalloproteinase 2(MMP-2), MMP-9, MMP-14, and tissue inhibitor of metalloproteinases 2(TIMP-2), focal adhesion kinase (FAK), ERK1/2, JNK and Src. Results: According to the expression levels of GRP78, MMP-2, MMP-9, MMP-14 and TIMP-1 in hepatocellular carcinoma cell lines SMMC7721 and hepG2, we used SMMC7721 as the in vitro invasion model for further functional analysis. Using this model, we found that GRP78 knockdown decreased the invasion of tumor cells, and this inhibitory effect was independent of cell proliferation. In hepatocellular carcinoma cells, Grp78 knockdown inhibited ECM degradation and the decreased activity and expression of MMP-2, but not MMP-9 contributed largely to this impact. Further analysis revealed that the decreased activity and expression of MMP-2 is mediated by JNK. Conclusion: Knockdown of GRP78 decreases ECM degradation, and downregulates the expression and activity of MMP-2 and TIMP-2. These results further demonstrate that GRP78 is a potential target for inhibiting the invasion of hepatocellular carcinoma cells. © 2012 Li et al.; licensee BioMed Central Ltd.
Tian B.,First Affiliated Hospital of Liaoning Medical College |
Zhang Y.,Jinzhou Central Hospital |
Li N.,Jinzhou Central Hospital
Cancer Biotherapy and Radiopharmaceuticals | Year: 2013
Objective: To study the expression and regulatory effects of CD146 protein in colorectal cancer and the correlation between CD146 protein expression and the prognosis of colorectal cancer. Materials and Methods: The CD146 protein level was detected by immunohistochemistry staining. The relationship between CD146 expression and clinicopathological parameters of colorectal cancer was determined. Results: It was observed that 216 (20.00%) of the 1080 cases positively expressed CD146 protein. Univariate analyses indicated that CD146 expression was related to histological grade, Duke's stage, and liver metastasis (p=0.001, 0.001, and 0.001, respectively). Spearman correlation analysis showed that CD146 expression has line correlation to histological grade, Duke's stage, and liver metastasis (p=0.02, 0.01 and 0.001, respectively). After multivariate analysis, Duke's stage and CD146 were related to liver metastasis (p=0.01 and 0.001, respectively). In the Cox regression test, histological grade, Duke's stage, and CD146 were detected as the independent prognostic factors (p=0.045, 0.01, and 0.001, respectively). Conclusions: CD146 protein may be a potential biomarker for the postoperative liver metastasis of colorectal cancer. © Copyright 2013, Mary Ann Liebert, Inc. 2013.
Xu J.,First Affiliated Hospital of Liaoning Medical College |
Li H.,Liaoning Medical College |
Gu L.,First Affiliated Hospital of Liaoning Medical College |
Zhou X.,First Affiliated Hospital of Liaoning Medical College
Tumor Biology | Year: 2014
Vitamin D receptor (VDR) poly(A) is a common genetic polymorphism in the VDR gene, and it has been implicated to be associated with breast cancer risk. However, previous studies on the association reported inconclusive results. We performed this meta-analysis to comprehensively assess the association. Eligible studies were searched in PubMed and EMBASE databases. Odds ratio (OR) and its 95 % confidence interval (95 % CI) were used for statistical analysis. A total 6,631 cases and 6,718 controls from 11 case-control studies were finally included into the meta-analysis. Meta-analysis of total eligible studies showed that VDR poly(A) polymorphism was not associated with the risk of breast cancer (S versus L: OR=0.99, 95 % CI of 0.90-1.09, P=0.84; SS versus LL: OR=0.96, 95%CI of 0.79-1.18, P=0.70; SS/LS versus LL: OR=0.96, 95%CI of 0.83-1.12, P=0.63; SS versus LL/LS: OR=1.00, 95 % CI of 0.91-1.10, P=0.98). Metaanalysis of studies with high quality also showed that there was no association between VDR poly(A) polymorphism and breast cancer risk. In addition, in the subgroup analysis by ethnicity, no significant association was found among Caucasians. Therefore, the meta-analysis suggests that VDR poly(A) polymorphism is not associated with the risk of breast cancer. Large well-designed studies are necessary to clarify the possible association in Asians.
Tian B.,First Affiliated Hospital of Liaoning Medical College
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine | Year: 2012
The expression status of CD74 in breast cancer stem cells and its clinical implications was evaluated in order to lay a foundation for managing breast cancer. Five hundred and eighty breast cancer specimens were enrolled in the study. The relationship between the CD74 protein and clinicopathological parameters as well as prognosis was subsequently determined. In total, 468 (80.69 %) of the 580 breast cases showed CD74-positive expression. After universal analysis, CD74 was observed to be related to lymph node metastasis and triple-negative breast cancer (P = 0.01 and 0.001). Moreover, CD74 expression has a line correlation with lymph node metastasis and triple-negative breast cancer (P = 0.02 and 0.001). Furthermore, periostin was shown to attain a significantly more distant liver metastasis and worse disease-specific survival than those with none or low-expressed CD74 protein (P = 0.001). In the Cox regression test, CD74 protein was detected as an independent prognostic factor (P = 0.001). CD74 is consistently expressed in triple-negative subgroups of breast cancer and might be a new potential marker for triple-negative breast cancer.
Zhang J.,First Affiliated Hospital of Liaoning Medical College
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery | Year: 2011
Exploring the characteristics of arytenoid cartilage movement. Using Pro/ENGINEER (Pro/E) software, the cricoid cartilage, arytenoid cartilage and vocal cords were simulated to the three-dimensional reconstruction, by analyzing the trajectory of arytenoid cartilage in the joint surface from the cricoid cartilage and arytenoid cartilage composition. The 3D animation simulation showed the normal movement patterns of the vocal cords and the characteristics of vocal cords movement in occasion of arytenoid cartilage dislocation vividly. The three-dimensional model has clinical significance for arytenoid cartilage movement disorders.
Zhang Y.,First Affiliated Hospital of Liaoning Medical College
International Journal of Ophthalmology | Year: 2011
AIM: To evaluate the effectiveness of pneumatic retinopexy(PR) with cryopexy in the therapy of inferior rhegmatogenous retinal detachment(RRD). METHODS: Twenty-two patients(22 eyes) were included in this study. The RRD diagnosis was confirmed by best-corrected visual acuity (BCVA), slit-lamp microscope, indirect ophthalmoscope and Goldman three-mirror contact lens. All patients had undergone PR with cryopexy, and the post-operative follow-up was 6 to 24 months with an average of 12 months. Post-operative BCVA and final anatomical outcome was analyzed. RESULTS: The reattachment rate of single PR was 77% (17/22 eyes), and 2 eyes required additional intravitreal injection of the gas. The total reattachment rate of PR was 86% (19/22 eyes). Three eyes needed additional scleral buckling. Final retinal reattachment was achieved in all 22 subjects (100%). Postoperative BCVA was significantly improved (P<0.01). CONCLUSION: PR is an effective surgery for inferior RRD.
[Comparative study on osteogenic effect of bone marrow mesenchymal stem cells transfected by adenovirus-bone morphogenetic protein 2-internal ribosome entry site-hypoxia inducible factor 1alpha(mu) and by bone morphogenetic protein 2 single gene].
Zhang J.,First Affiliated Hospital of Liaoning Medical College
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery | Year: 2012
To compare the osteogenic effect of bone marrow mesenchymal stem cells (BMSCs) transfected by adenovirus-bone morphogenetic protein 2-internal ribosome entry site-hypoxia inducible factor 1alpha(mu) (Ad-BMP-2-IRES-HIF-1alpha(mu)) and by Ad-cytomegalovirus (CMV)-BMP-2-IRES-human renilla reniformis green fluorescent protein 1 (hrGFP-1) single gene so as to optimize the source of osteoblasts. BMSCs were separated and cultured from 1-month-old New Zealand white rabbit. The BMSCs at passage 3 were transfected by virus. The experiment was divided into 4 groups (groups A, B, C, and D) according to different virus: BMSCs were transfected by Ad-BMP-2-IRES-HIF-1alpha(mu) in group A, by Ad-CMV-BMP-2-IRES-hrGFP-1 in group B, by Ad-CMV-IRES-hrGFP-1 in group C, and BMSCs were not transfected in group D. The optimum multiplicity of infection (MOI) (50, 100, 150, and 200) was calculated and then the cells were transfected by the optimum MOI, respectively. The expression of BMP-2 gene was detected by immunohistochemistry staining after transfected, the expressions of BMP-2 protein and HIF-1alpha protein were detected by Western blot method. The osteogenic differentiation potential was detected by alkaline phosphatase (ALP) activity and Alizarin red staining. The optimum MOI of groups A, B, and C was 200, 150, and 100, respectively. The expression of BMP-2 was positive in groups A and B, and was negative in groups C and D by immunohistochemistry staining; the number of positive cells in group A was more than that in group B (P < 0.05). The expression of BMP-2 protein in groups A and B was significantly higher than that in groups C and D (P < 0.05), group A was higher than group B (P < 0.05). The expression of HIF-1alpha protein in group A was significantly higher than those in the other 3 groups (P < 0.05), no significant difference was found among the other 3 groups (P > 0.05). ALP activity in groups A and B was significantly higher than that in groups C and D (P < 0.05), group A was higher than group B (P < 0.05). Calcium nodules could be seen in groups A and B, but not in groups C and D; the number of calcium nodules in group A was higher than that in group B (P < 0.05). The expression of BMP-2 and osteogenic effect of BMSCs transfected by Ad-BMP-2-IRES-HIF-1alpha(mu) (double genes in single carrier) are higher than those of BMSCs transfected by Ad-CMV-BMP-2-IRES-hrGFP-1 (one gene in single carrier).
Liu H.-Y.,First Affiliated Hospital of Liaoning Medical College |
Dong Z.,First Affiliated Hospital of Liaoning Medical College
OncoTargets and Therapy | Year: 2014
Ovarian cancer is a leading gynecological malignancy associated with high mortality. Hedgehog signaling has been found to be important for cell proliferation and tumor growth for multiple cancers, including ovarian cancer. The present study showed that the drug cyclopamine, which blocks the hedgehog signaling pathway, could reduce cancer cell growth and proliferation and induce cell apoptosis. In addition, the silencing of the glioma-associated oncogene (Gli)3, a downstream component of the hedgehog signaling pathway, could further enhance the antitumor effects of cyclopamine. Our results suggest that Gli3 may act as resistance to cyclopamine‘s effect on tumor growth. The combined treatment of cyclopamine application and Gli3 silencing therapy, therefore, may provide novel directions for clinical management of ovarian cancer. © 2014 Liu and Dong.