Affiliated Calmette Hospital of Kun Ming Medical University

Kunming, China

Affiliated Calmette Hospital of Kun Ming Medical University

Kunming, China
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Zhang L.,Affiliated Calmette Hospital of Kun Ming Medical University | Zhao Y.-H.,Affiliated Calmette Hospital of Kun Ming Medical University | Guan Z.,Affiliated Calmette Hospital of Kun Ming Medical University | Ye J.-S.,Affiliated Calmette Hospital of Kun Ming Medical University | And 3 more authors.
Bio-Medical Materials and Engineering | Year: 2015

The shortage of organ resource has been limiting the application of liver transplantation. Bioartificial liver construction is increasingly focused as a replacement treatment. To product a bioartificial liver, three elements must be considered: seeding cells, scaffold and bioreactor. Recent studies have shown that several methods can successfully differentiate MSC (mesenchymal stem cells) derived from Wharton's jelly into hepatocyte, such as stimulating MSC by cytokines and growth factors, direct and indirect co-culture MSC with hepatocytes, or promote MSC differentiation by 3-dimensional matrix. In some cases, differentiation of MSC into hepatocytes can also be an alternative approach for whole organ transplantation in treatment of acute and chronic liver diseases. In this review, the characterization of MSC from Wharton's jelly, their potential of application in liver tissue engineering on base of decellularized scaffold, their status of banking and their preclinical work performed will be discussed. © 2015 - IOS Press and the authors.


Ye J.-S.,Affiliated Calmette Hospital of Kun Ming Medical University | Ye J.-S.,University of Lorraine | Stoltz J.-F.,University of Lorraine | De Isla N.,University of Lorraine | And 3 more authors.
Bio-Medical Materials and Engineering | Year: 2015

OBJECTIVES: In present study, we plan to produce a decellularization protocol from rat liver to generate a three-dimensional whole organ scaffold.METHODS: A combination of 1% SDS and 1% tritonX-100 were used orderly to decellularize rat livers. After about 6 h of interactive antegrade/retrograde perfusion, a decellularized whole translucent liver scaffold with integrated blood vessel networks was generated. The decellularized livers are charactered by light microscopy, scanning electron microscopy, and biochemical analysis (DNA quantification) for preservation of the three-dimension of extracellular matrix architecture.RESULTS: The decellularization protocol was verified by observation of the whole translucent liver organ with intact vascular trees under macroscopy, in conjunction with the hematoxylin-eosin staining that showed no cells or nuclear material remained. Additionally, the Masson's stain indicted that the extracellular proteins were well kept and scanning electron microscopy (SEM) revealed a preserved decellularized matrix architecture. Compared to normal livers, DNA in the decellularized livers was quantified less than 10% at the same mass.CONCLUSIONS: The current method of decellularization protocol was feasible, simple and quick, and was verified by an absence of residual cells. The decellularized extracellular matrix had preserved integrate vascular network and a three-dimensional architecture. © 2015 - IOS Press and the authors.

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