Aerosol Research Laboratory
Aerosol Research Laboratory
Gilani K.,Aerosol Research Laboratory |
Gilani K.,Tehran University of Medical Sciences |
Daman Z.,Aerosol Research Laboratory |
Moazeni E.,Aerosol Research Laboratory |
And 4 more authors.
Journal of Drug Delivery Science and Technology | Year: 2014
This study investigated various solid dispersions of itraconazole with different carrier blends to enhance its dissolution. HPMC E5, HPMC E15, PEG 20000 and TPGS 1000 were used to construct 20 % drug containing solid matrices by spray drying method. The formulations were analyzed in terms of dissolution profile, x-ray diffraction (XRD) and/or differential scanning calorunetiy (DSC) pattern once immediately after preparation and again after storage for three months at accelerated conditions. The results showed that different excipients could put their effects on the dissolution behavior of the drug mainly through affecting the solid states of the resultant mixtures. In this regard, presence of high portions of HPMC in the resultant matrices was the most promising approach for improving the dissolution rate of itraconazole. The dissolution behavior of the drug after storage was directly related to its relative crystal growth during this period, since the more generated semicrystalline structures showed slower dissolution rates.
Shokri Z.,Islamic Azad University at Tehran |
Fazeli M.R.,Probiotic Research Laboratory |
Ardjmand M.,Islamic Azad University at Tehran |
Mousavi S.M.,Tarbiat Modares University |
Gilani K.,Aerosol Research Laboratory
DARU, Journal of Pharmaceutical Sciences | Year: 2015
Background: There is substantial clinical data supporting the role of Bifidobacterium bifidum in human health particularly in benefiting the immune system and suppressing intestinal infections. Compared to the traditional lyophilization, spray-drying is an economical process for preparing large quantities of viable microorganisms. The technique offers high production rates and low operating costs but is not usually used for drying of substances prone to high temperature. The aim of this study was to establish the optimized environmental factors in spray drying of cultured bifidobacteria to obtain a viable and stable powder. Methods: The experiments were designed to test variables such as inlet air temperature, air pressure and also maltodextrin content. The combined effect of these variables on survival rateand moisture content of bacterial powder was studied using a central composite design (CCD). Sub-lethal heat-adaptation of a B. bifidum strain which was previously adapted to acid-bile-NaCl led to much more resistance to high outlet temperature during spray drying. The resistant B. bifidum was supplemented with cost friendly permeate, sucrose, yeast extract and different amount of maltodextrin before it was fed into a Buchi B-191 mini spray-dryer. Results: Second-order polynomials were established to identify the relationship between the responses andthe three variables. Results of verification experiments and predicted values from fitted correlations were in close agreement at 95% confidence interval. The optimal values of the variables for maximum survival and minimum moisture content of B. bifidum powder were as follows: inlet air temperature of 111.15°C, air pressure of 4.5 bar and maltodextrin concentration of 6%. Under optimum conditions, the maximum survival of 28.38% was achieved while moisture was maintained at 4.05%. Conclusion: Viable and cost effective spray drying of Bifidobacterium bifidum could be achieved by cultivating heat and acid adapted strain into the culture media containing nutritional protective agents. © 2015 Shokri et al.; licensee BioMed Central.
Geller D.E.,Aerosol Research Laboratory |
Kesser K.C.,Aerosol Research Laboratory
Journal of Aerosol Medicine and Pulmonary Drug Delivery | Year: 2010
Background: Inhaled α1-antitrypsin (AAT) is being developed for treatment of cystic fibrosis to protect the lungs from excessive free elastase. High drug costs mandate a very efficient aerosol system to deliver a high payload to the airways. The I-neb Adaptive Aerosol Delivery (AAD) System is a portable, electronic, vibrating mesh nebulizer that delivers aerosol only during inhalation. It can be operated in conventional tidal breathing mode (TBM) or in target inhalation mode (TIM) that guides the patient to inhale deeply and slowly. The purposes of this in vitro study were to determine aerosol characteristics, device efficiency, and delivery time of AAT using the I-neb AAD System with TBM and TIM. Methods: We studied the I-neb AAD System in TBM and TIM (inspiratory time 6 or 9 sec) using a breath simulator. The loaded dose was 0.5 mL AAT (50 mg/mL). Nebulized drug captured on an inspiratory filter was reported as emitted dose. Particle size was measured by laser diffraction. Predicted lung doses were calculated based on the results of a prior scintigraphy study of the I-neb AAD System. Results: Particle size (VMD) for TBM and TIM was similar (4.4-4.8 μm). The emitted doses were very high and similar between modes (82-90% of loaded dose). Predicted lung dose of AAT (percent of loaded dose) and delivery times were: TBM 56.6% in 7.5 min; TIM-6 59.9% in 4.4 min; and TIM-9 64.5% in 2.5 min. Conclusions: The I-neb AAD System enhanced AAT delivery by inhalation-only aerosol generation and a low-residual dose. Predicted lung dose was high for both TBM and TIM, but longer inspiratory times with TIM reduced the administration time to one-third that of tidal breathing. We conclude that slow, deep, controlled inspirations using the I-neb AAD System is an efficient method to deliver AAT. © Copyright 2010, Mary Ann Liebert, Inc. 2010.
Geller D.E.,Aerosol Research Laboratory |
Weers J.,Novartis |
Journal of Aerosol Medicine and Pulmonary Drug Delivery | Year: 2011
At present, the only approved inhaled antipseudomonal antibiotics for chronic pulmonary infections in patients with cystic fibrosis (CF) are nebulized solutions. However, prolonged administration and cleaning times, high administration frequency, and cumbersome delivery technologies with nebulizers add to the high treatment burden in this patient population. PulmoSphere™ technology is an emulsion-based spray-drying process that enables the production of light porous particle, dry-powder formulations, which exhibit improved flow and dispersion from passive dry powder inhalers. This review explores the fundamental characteristics of PulmoSphere technology, focusing on the development of a dry powder formulation of tobramycin for the treatment of chronic pulmonary Pseudomonas aeruginosa (Pa) infection in CF patients. This dry powder formulation provides substantially improved intrapulmonary deposition efficiency, faster delivery, and more convenient administration over nebulized formulations. The availability of more efficient and convenient treatment options may improve treatment compliance, and thereby therapeutic outcomes in CF. © 2011 Mary Ann Liebert, Inc.
Kalantarian P.,Aerosol Research Laboratory |
Kalantarian P.,Tehran University of Medical Sciences |
Najafabadi A.R.,Aerosol Research Laboratory |
Haririan I.,Tehran University of Medical Sciences |
And 4 more authors.
International Journal of Nanomedicine | Year: 2010
This study concerns the supercritical antisolvent process which allows single-step production of 5-fluorouracil (5-FU) nanoparticles. This process enhances the physical characteristics of 5-FU in order to deliver it directly to the respiratory tract. Several mixtures of methanol with dichloromethane, acetone, or ethanol were used for particle preparation, and their effects on the physical characteristics of the final products were studied. The conditions of the experiment included pressures of 100 and 150 bar, temperature of 40°C, and a flow rate of 1 mL/min. The particles were characterized physicochemically before and after the process for their morphology and crystallinity. In spite of differences in size, the particles were not very different regarding their morphology. The resulting particles were of a regular shape, partly spherical, and appeared to have a smooth surface, whereas the mechanically milled particles showed less uniformity, had surface irregularities and a high particle size distribution, and seemed aggregated. Particles of 5-FU precipitated from methanol-dichloromethane 50:50 had a mean particle size of 248 nm. In order to evaluate the aerodynamic behavior of the nanoparticles, six 5-FU dry powder formulations containing mixtures of coarse and fine lactose of different percentages were prepared. Deposition of 5-FU was measured using a twin-stage liquid impinger and analyzed using a validated high pressure liquid chromatography method. Addition of fine lactose improved the aerodynamic performance of the drug, as determined by the fine particle fraction. © 2010 Kalantarian et al, publisher and licensee Dove Medical Press Ltd.