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Fremont, CA, United States

Patent
Aemtek Inc. and The United States Of America As Represented By The Secretary Of Agriculture | Date: 2015-02-18

Systems and methods for sampling within a conveyance system are provided herein, such systems and methods being particularly useful in batch sampling of food products for a targeted biological agent. In one aspect, the sampling system includes a conveyance system and one or more sampling devices positioned along a conveyance path such that at least one portion of the batch contacts a sampling medium of the one or more sampling devices. In another aspect, sampling devices are provided that allow a sampling member to be secured in a sampling position for batch testing and readily removed after sampling and tested. The systems, devices and methods herein provide improved sampling coverage of the entire batch and reduce waste and inefficiency as compared to conventional batch sampling methods.


Patent
Aemtek Inc. | Date: 2013-01-18

A sample holding device having a plurality of sample wells. A gutter surrounds the sample wells and is in fluidic communication with an overflow sample well. An upper surface of the device is sealed with a sealing film that fluidically isolates each sample well from each other and from the gutter and overflow well. The device is used to perform a most probable number (MPN) procedure.


Bird P.,Q Laboratories Inc. | Flannery J.,Q Laboratories Inc. | Crowley E.,Q Laboratories Inc. | Agin J.,Q Laboratories Inc. | And 32 more authors.
Journal of AOAC International | Year: 2015

The 3M™ Molecular Detection Assay (MDA) Listeria monocytogenes combines isothermal amplification and bioluminescence to detect Listeria monocytogenes with high specificity and efficiency in select foods and environmental samples. The 3M MDA Listeria monocytogenes method was evaluated using an unpaired study design in a multilaboratory collaborative study to the U.S. Department of Agriculture, Food Safety and Inspection Service Microbiology Laboratory Guidebook 8.09 (2011) Isolation and Identification of Listeria monocytogenes from Red Meat, Poultry, and Egg Products and Environmental Samples for deli Turkey, and the AOAC Official Method of AnalysisSM 993.12 Listeria monocytogenes in Milk and Dairy Products for full-fat (4% milk fat) cottage cheese following the current AOAC guidelines. A total of 16 laboratories located in the continental United States and Canada participated in this collaborative study. For deli Turkey, 125 g test portions were evaluated using heat-stressed cells by each method. For full-fat cottage cheese, 25 g test portions were evaluated using nonheatstressed cells. Each matrix had three inoculation levels: an uninoculated control level (0 CFU/test portion), and two levels artificially contaminated with L. monocytogenes, a low inoculum level (0.2-2 CFU/test portion) and a high inoculum level (2-5 CFU/test portion). In total, 1584 unpaired replicate samples were analyzed. Statistical analysis was conducted according to the probability of detection (POD) model. Results obtained for the low inoculum level full-fat cottage cheese test portions produced a difference in cross-laboratory POD (dLPOD) value of -0.08 with a 95% confidence interval (CI) of (-0.20, 0.05). For the low-level deli Turkey test portions, a dLPOD value of -0.02 with a 95% CI of (-0.14, 0.11) was obtained. Source


Bird P.,Q Laboratories Inc. | Flannery J.,Q Laboratories Inc. | Crowley E.,Q Laboratories Inc. | Agin J.,Q Laboratories Inc. | And 29 more authors.
Journal of AOAC International | Year: 2015

The 3M™ Molecular Detection Assay (MDA) Listeria is used with the 3M Molecular Detection System for the detection of Listeria species in food, foodrelated, and environmental samples after enrichment. The assay utilizes loop-mediated isothermal amplification to rapidly amplify Listeria target DNA with high specificity and sensitivity, combined with bioluminescence to detect the amplification. The 3M MDA Listeria method was evaluated using an unpaired study design in a multilaboratory collaborative study and compared to the AOAC Official Method of AnalysisSM (OMA) 993.12 Listeria monocytogenes in Milk and Dairy Products reference method for the detection of Listeria species in full-fat (4% milk fat) cottage cheese (25 g test portions). A total of 15 laboratories located in the continental United States and Canada participated. Each matrix had three inoculation levels: an uninoculated control level (0 CFU/test portion), and two levels artificially contaminated with Listeria monocytogenes, a low inoculum level (0.2-2 CFU/test portion) and a high inoculum level (2-5 CFU/test portion) using nonheatstressed cells. In total, 792 unpaired replicate portions were analyzed. Statistical analysis was conducted according to the probability of detection (POD) model. Results obtained for the low inoculum level test portions produced a difference in crosslaboratory POD value of -0.07 with a 95% confidence interval of (-0.19, 0.06). No statistically significant differences were observed in the number of positive samples detected by the 3M MDA Listeria method versus the AOAC OMA method. Source

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