Puting Kahoy, Philippines

Adventist University of the Philippines

www.aup.edu.ph
Puting Kahoy, Philippines

The Adventist University of the Philippines is a private coeducational Christian university located in Puting Kahoy, Silang, Cavite, Philippines. The university is affiliated with the Seventh-day Adventist Church and holds an autonomous status recognized by the CHED . Wikipedia.


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Ono J.,Chiba University | Yong J.W.H.,Singapore University of Technology and Design | Takayama K.,Museum of Natural and Environmental History | Saleh M.N.B.,University Putra Malaysia | And 13 more authors.
Conservation Genetics | Year: 2016

Bruguiera hainesii (Rhizophoraceae) is one of the two Critically Endangered mangrove species listed in the IUCN Red List of Threatened Species. Although the species is vulnerable to extinction, its genetic diversity and the evolutionary relationships with other Bruguiera species are not well understood. Also, intermediate morphological characters imply that the species might be of hybrid origin. To clarify the genetic relationship between B. hainesii and other Bruguiera species, we conducted molecular analyses including all six Bruguiera species using DNA sequences of two nuclear genes (CesA and UNK) and three chloroplast regions (intergenic spacer regions of trnL-trnF, trnS-trnG and atpB-rbcL). For nuclear DNA markers, all nine B. hainesii samples from five populations were heterozygous at both loci, with one allele was shared with B. cylindrica, and the other with B. gymnorhiza. For chloroplast DNA markers, the two haplotypes found in B. hainesii were shared only by B. cylindrica. These results suggested that B. hainesii is a hybrid between B. cylindrica as the maternal parent and B. gymnorhiza as the paternal one. Furthermore, chloroplast DNA haplotypes found in B. hainesii suggest that hybridization has occurred independently in regions where the distribution ranges of the parental species meet. As the IUCN Red List of Threatened Species currently excludes hybrids (except for apomictic plant hybrids), the conservation status of B. hainesii should be reconsidered. © 2016 Springer Science+Business Media Dordrecht


Tomizawa Y.,Chiba University | Shinmura Y.,Chiba University | Wee A.K.S.,National University of Singapore | Takayama K.,University of Tokyo | And 18 more authors.
Conservation Genetics Resources | Year: 2013

Human impacts have seriously damaged mangroves, and conservation of mangroves will require information on local and regional population genetic structures. Here, we report the development and polymorphism of eleven novel microsatellite markers, developed using next-generation sequencing on 56 samples of widespread mangrove species Xylocarpus granatum (Meliaceae) from nine populations across the Indo-West Pacific region. All loci were found to be polymorphic, with the number of alleles per locus ranging from four to 19. In a population from Sabah (Malaysia), the mean observed and expected heterozygosity per locus was 0.59 and 0.58, respectively. No null allele, significant linkage disequilibrium or deviation from Hardy-Weinberg equilibrium was detected among all loci. The eleven markers developed can be valuable tools to conservation genetics of this species across its distributional range. © 2013 Springer Science+Business Media Dordrecht.


Shinmura Y.,Chiba University | Wee A.K.S.,National University of Singapore | Takayama K.,University of Vienna | Asakawa T.,Chiba University | And 17 more authors.
Conservation Genetics Resources | Year: 2012

A set of 15 new microsatellite loci was developed and characterized for the widespread mangrove tree species Sonneratia alba (Lythraceae) by using next-generation sequencing. Forty-eight S. alba samples from seven populations in the Indo-West Pacific region were genotyped; all loci were polymorphic, with the number of alleles ranging from three to eight. The mean observed heterozygosity per locus was 0.21 for a population from Sabah, Malaysia. No null allele or significant linkage disequilibrium was detected, indicating the robustness of the markers. Only one locus (SA103) showed deviation from Hardy-Weinberg equilibrium. As characterization of these microsatellite loci was done with samples covering most of the species' distribution range, the markers can be applied to genetic diversity studies over the broad geographical range of the species. © 2012 Springer Science+Business Media B.V.


Shinmura Y.,Chiba University | Wee A.K.S.,National University of Singapore | Takayama K.,University of Vienna | Meenakshisundaram S.H.,Ms Swaminathan Research Foundation | And 17 more authors.
Conservation Genetics Resources | Year: 2012

A set of 14 new microsatellite markers was developed for mangrove species Rhizophora mucronata (Rhizophoraceae) by using pyrosequencing. Fifty-six samples from 9 populations of R. mucronata in the Indo-West Pacific region were genotyped; all loci were polymorphic, with the number of alleles ranging from 2 to 9. The mean expected heterozygosity per locus was 0. 16 in a population from Sabah, no significant linkage disequilibrium was found among loci, and significant deviation from Hardy-Weinberg equilibrium was found in 3 loci. The polymorphic microsatellite markers with samples covering most of the species' distribution range can be applied in genetic diversity studies covering a broad geographical range of the species. © 2012 Springer Science+Business Media B.V.


Wee A.K.S.,Chiba University | Wee A.K.S.,CAS Xishuangbanna Tropical Botanical Garden | Takayama K.,University of Tokyo | Chua J.L.,National University of Singapore | And 16 more authors.
BMC Evolutionary Biology | Year: 2015

Background: Mangrove forests are ecologically important but globally threatened intertidal plant communities. Effective mangrove conservation requires the determination of species identity, management units, and genetic structure. Here, we investigate the genetic distinctiveness and genetic structure of an iconic but yet taxonomically confusing species complex Rhizophora mucronata and R. stylosa across their distributional range, by employing a suite of 20 informative nuclear SSR markers. Results: Our results demonstrated the general genetic distinctiveness of R. mucronata and R. stylosa, and potential hybridization or introgression between them. We investigated the population genetics of each species without the putative hybrids, and found strong genetic structure between oceanic regions in both R. mucronata and R. stylosa. In R. mucronata, a strong divergence was detected between populations from the Indian Ocean region (Indian Ocean and Andaman Sea) and the Pacific Ocean region (Malacca Strait, South China Sea and Northwest Pacific Ocean). In R. stylosa, the genetic break was located more eastward, between populations from South and East China Sea and populations from the Southwest Pacific Ocean. The location of these genetic breaks coincided with the boundaries of oceanic currents, thus suggesting that oceanic circulation patterns might have acted as a cryptic barrier to gene flow. Conclusions: Our findings have important implications on the conservation of mangroves, especially relating to replanting efforts and the definition of evolutionary significant units in Rhizophora species. We outlined the genetic structure and identified geographical areas that require further investigations for both R. mucronata and R. stylosa. These results serve as the foundation for the conservation genetics of R. mucronata and R. stylosa and highlighted the need to recognize the genetic distinctiveness of closely-related species, determine their respective genetic structure, and avoid artificially promoting hybridization in mangrove restoration programmes. © 2015 Wee et al.; licensee BioMed Central.


PubMed | University of Kota, Yangon University, University of Ryukyus, University of North Sumatra and 11 more.
Type: | Journal: BMC evolutionary biology | Year: 2015

Mangrove forests are ecologically important but globally threatened intertidal plant communities. Effective mangrove conservation requires the determination of species identity, management units, and genetic structure. Here, we investigate the genetic distinctiveness and genetic structure of an iconic but yet taxonomically confusing species complex Rhizophora mucronata and R. stylosa across their distributional range, by employing a suite of 20 informative nuclear SSR markers.Our results demonstrated the general genetic distinctiveness of R. mucronata and R. stylosa, and potential hybridization or introgression between them. We investigated the population genetics of each species without the putative hybrids, and found strong genetic structure between oceanic regions in both R. mucronata and R. stylosa. In R. mucronata, a strong divergence was detected between populations from the Indian Ocean region (Indian Ocean and Andaman Sea) and the Pacific Ocean region (Malacca Strait, South China Sea and Northwest Pacific Ocean). In R. stylosa, the genetic break was located more eastward, between populations from South and East China Sea and populations from the Southwest Pacific Ocean. The location of these genetic breaks coincided with the boundaries of oceanic currents, thus suggesting that oceanic circulation patterns might have acted as a cryptic barrier to gene flow.Our findings have important implications on the conservation of mangroves, especially relating to replanting efforts and the definition of evolutionary significant units in Rhizophora species. We outlined the genetic structure and identified geographical areas that require further investigations for both R. mucronata and R. stylosa. These results serve as the foundation for the conservation genetics of R. mucronata and R. stylosa and highlighted the need to recognize the genetic distinctiveness of closely-related species, determine their respective genetic structure, and avoid artificially promoting hybridization in mangrove restoration programmes.


Wittayawannakul W.,University of the Philippines at Los Baños | Garcia R.N.,University of the Philippines at Los Baños | Yllano O.B.,University of the Philippines at Los Baños | Yllano O.B.,Adventist University of the Philippines | And 3 more authors.
Philippine Agricultural Scientist | Year: 2010

The genetic diversity of 22 accessions of the genus Garcinia was assessed using peroxidase, RAPD markers and gene sequence specific amplification polymorphism (GSSAP). Among the 15 isozymes tested, only peroxidase produced reproducible, polymorphic bands with a polymorphism information content (PIC) of 0.79. A total of eight bands were generated forming three fingerprint patterns distinct for G. mangostana, G. binucao, G. kydia and G. lateriflora. No bands were observed for G. livingstonei and G. xanthochymus. The three RAPD primers showed high PIC of 0.92 (OPB-04), 0.78 (OPB-06) and 0.91 (OPB-07). For GSSAP markers, two sets of primers based on the conserved regions of acyl-ACP thioesterase (ACYL-ACP), and chalcone synthase (CHALCS) had relative PICs of 0.75 and 0.89 for ACYL-ACP and CHALCS, respectively. The high PICs indicate the capability of these techniques to quantify genetic diversity in Garcinia species. The dendrograms using UPGMA-SAHN cluster analysis based on peroxidase, RAPD and GSS amplification polymorphism showed that Garcinia species clustered into five groups at mean similarity coefficient 0.54. Group I consisted of all 17 G. mangostana accessions and was further classified into three subgroups (Ia, Ib and Ic). Group II composed of G. kydia and G. lateriflora showed a genetic similarity of 0.94. G. livingstonei, G. xanthochymus and G. binucao were unique in their groups. This study showed that the G. mangostana accessions analyzed had low genetic variation and that the different species can be clearly distinguished by combined peroxidase, RAPD and gene sequence specific amplification polymorphism.


Aclan E.M.,Adventist University of the Philippines | Aziz N.H.A.,Northern University of Malaysia
International Journal of Applied Linguistics and English Literature | Year: 2015

To survive in the 21st century workplace, communication skills are extremely important. However, a mismatch between the industry requirement and the university graduates’ competencies in terms of effective communication skills exists. Rote learning and lack of opportunities to practice English communication skills inside and outside the classroom are common issues in EFL/ESL contexts. Thus, this qualitative study was conducted to explore how debate as a pedagogical tool with three stages - pre-debate, actual debate and post-debate - can develop communication skills. The data were gathered through semi-structured one-on-one interview with five debate experts across from ASEAN countries and focus group interview with six ASEAN debate students. The participants of this study described the use of the pre-debate stage for the research and brainstorming tasks that engage the team members with each other, the actual debate for the arguments, POI and rebuttals that actively engage debaters with their opponents, and the postdebate stage that engage all the debaters with the adjudicators, their team-mates and their opponents. This pedagogical aspect focusing on the three stages of debate which has implications for SLA and language teaching was not substantially dealt with in previous studies on debate. © Australian International Academic Centre, Australia.


Macalalad M.B.,Adventist University of the Philippines | Narbarte M.P.,Adventist University of the Philippines
American Society of Agricultural and Biological Engineers Annual International Meeting 2015 | Year: 2015

This study determined to know the objectives, procedures, and feedbacks from creditors and suppliers of goods and services and from department heads/workers involved; to analyze its strengths and weaknesses; to identify the problems encountered, suggest solutions in the procedures, and to make recommendations for modification and improvement of funds disbursement. The purpose is to achieve a more tight control over funds and provide administrative techniques to satisfy the needs of creditors and suppliers in the collection of payments of goods and services delivered in a private hospital. Twenty (20) creditors and suppliers of goods and services and fifteen (15) department heads/workers involved, with a total of 35 respondents participated in answering the survey questionnaires. The President, the Vice-President for Finance and her Secretary, the Comptroller, and the Accountant were the respondents to the five sets of structured interview questionnaire respectively. A majority of creditors and suppliers, and departments heads/workers involved in the disbursement of funds in a private hospital were satisfied with their procedures; however, a private hospital are not totally following the disbursement cycle controls. There is still a need to further strengthen and update proper disbursement scheme to better safeguard the funds and promote better relations and satisfaction with its creditors and suppliers, employees and the general public. Similar studies on funds disbursement of private and public hospitals in the local and foreign setting be conducted. Variables should be included in the profile of respondents in related studies of funds disbursement. Copyright © (2015) by the American Society of Agricultural & Biological Engineers All rights reserved.


Taclan L.B.,Adventist University of the Philippines | Agulto I.C.,Central Luzon State University | Taclan E.Y.,Adventist University of the Philippines | Abuel E.,Adventist University of the Philippines | And 2 more authors.
American Society of Agricultural and Biological Engineers Annual International Meeting 2015 | Year: 2015

The study was conducted in a coastal barangay in Pasuquin, Ilocos Norte, Philippines with pre determined high saltwater content (> 1000.0 ppm) of the groundwater system. A matrix of 5 deepwells and 25 shallow tubewells were constructed in a 7.5 ha agricultural production area. The 5 deepwells were used for the injection and addition of freshwater, while, the 25 wells were the obseivation wells. Electrical conductivity (EC) and depth to water table were measured and recorded daily for 6 months. Results of three-batches: single well, three-well and five- well of freshwater recharging process showed that the hydraulic barrier created during the single- well injection process has its sphere of influence only at the point of injection. This would mean that the hydraulic barrier formed had push more saltwater landwards, thus increased in groundwater salinity in the study area. The outcome of the second freshwater addition resulted to the formation of a hydraulic barrier in three locations resulted in an increased pressure that served as a driving force pushing salt ions to the edges of the groundwater system. Migration of salts through the effects of diffusion and dispersion cause the increase in saline concentration. The live-well process demonstrated the best freshwater addition process. Results of the addition of freshwater simultaneously to five injection wells resulted to decreased salinity was maintained considerably at low levels in the whole study area. Copyright © (2015) by the American Society of Agricultural & Biological Engineers.

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