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Bromsgrove, United Kingdom

Singh R.S.,Punjabi University | Saini G.K.,Punjabi University | Kennedy J.F.,Advanced Science and Technology Institute
Carbohydrate Polymers | Year: 2010

Pullulanase showed significant improvement in thermal stability after covalent immobilization on Duolite XAD761. Optimum temperature of immobilized enzyme was 60 °C, which was 10 °C higher than the free enzyme. Apparent Km values for pullulan, soluble starch and dextran were 4.4, 20 and 50 mg mL-1, respectively. Mn2+ and Ca2+ showed 2.0- and 2.2-fold increase in enzyme activity. Activation energy (Ea) of immobilized biocatalyst was 22.38 kJ mol-1. Thermodynamic parameters (ΔH*, ΔG*, ΔS*) for irreversible inactivation of immobilized pullulanase at different temperatures (60-70 °C) were also determined. D-value was maximum (95.95 h) at 60 °C and temperature quotient (Q10) was 1.29. Immobilized biocatalyst was effectively used for pullulan hydrolysis in a batch system. Stationary phase in hydrolysis (95.70 ± 1.36%) was reached after 300 min at 125 rpm. Pullulan hydrolysis yielded 4.21 ± 0.06% reducing sugars under optimal conditions. Immobilized biocatalyst was successfully recycled for 33 batches, but the enzyme activity was reduced to half after 25th cycle. © 2010 Elsevier Ltd. All rights reserved. Source


Singh R.S.,Punjabi University | Saini G.K.,Punjabi University | Kennedy J.F.,Advanced Science and Technology Institute
Carbohydrate Polymers | Year: 2010

Pullulan production was carried out at shake-flask level and purified with isopropanol precipitation using single-step purification strategy. Purified pullulan obtained was used for preparing maltotriose syrup using pullulanase. Pullulanase was subjected to kinetic and thermodynamic characterization before its use for pullulan hydrolysis. Pullulanase exhibited considerable activity between pH 5 and 6, with an optimum pH of 5.0. It remained stable (100%) for 6 h at optimum temperature of 50 °C. Mn2+ and Ca2+ showed 1.8 and 2.1 times increase in pullulanase activity. Pullulanase effectively hydrolyzed pullulan, soluble starch and dextran. Decimal reduction time (D value) was 76.77 h at 50 °C and Z value was 12.5 °C for pullulanase. Thermodynamic parameters (ΔH*, ΔG*, ΔS*) for irreversible inactivation of free pullulanase at different temperatures (50-70 °C) were also determined. In a batch system, pullulanase hydrolyzed 94.25 ± 1.83% of purified pullulan and the resultant syrup contained 3.77 ± 0.07 mg/mL of maltotriose. Thus far, there is no report on thermodynamics of irreversible inactivation of pullulanase for the hydrolysis of pullulan. © 2009 Elsevier Ltd. All rights reserved. Source


Singh R.S.,Punjabi University | Saini G.K.,Punjabi University | Kennedy J.F.,Advanced Science and Technology Institute
Carbohydrate Polymers | Year: 2011

Pullulanase covalently immobilized on Duolite XAD761 has been used for developing a continuous flow reactor for the hydrolysis of pullulan. Packed bed reactor containing 34.10 U of immobilized biocatalyst with feeding of pullulan solution (0.44%, w/v) at a flow rate of 5 mL h-1 supported maximum pullulan hydrolysis at 60 °C. The reactor was run continuously for 32 days and immobilized biocatalyst lost half of its original activity after 31 days of continuous operation at 60 °C. The volumetric productivity and yield of reducing sugars were 3.38 ± 0.02 g L-1 h-1 and 4.40 ± 0.01 mg mL-1, respectively during the beginning of the hydrolysis. The developed immobilized biocatalyst has shown good operational and mechanical stability and can be successfully used for the hydrolysis of pullulan in a continuous system. Literature survey reveals no report on continuous hydrolysis of pullulan using whole cells or immobilized enzyme. © 2010 Elsevier Ltd. All rights reserved. Source


Grant
Agency: Cordis | Branch: FP7 | Program: CSA | Phase: ICT-2007.9.1 | Award Amount: 839.91K | Year: 2007

The SEACOOP project addresses the development of SandT cooperation on ICT with Southeast Asia, a region of strategic interest for the European Union. Its two main objectives are to identify, promote, and support strategic cooperation opportunities, and to support bilateral and multilateral dialogues. The project also aims at developing awareness on SandT cooperation on ICT under FP7 in Southeast Asia, and at widely disseminating project perspectives and results in the European and Southeast Asian ICT communities. The SEACOOP activities build upon the achievements reached by the BASIC, ENGAGE, and GAPFILL FP6 SSAs, and will develop synergies with other programmes and initiatives similarly addressing EU-Southeast Asia cooperation on ICT. Project outputs will include: - The identification of, and support to 10 to 15 key opportunities of strategic cooperation, transformed, before the end of the project (18 months), into major cooperation initiatives between the two regions, - The organisation of a cooperation event in Europe and of awareness workshops in Vietnam, Cambodia, and Laos (or Brunei), - The development of a web-based portal devoted to EU-Southeast Asia cooperation on ICT, part of an extensive set of promotion and dissemination activities. The SEACOOP project is coordinated by Orionis, who has successfully coordinated the GAPFILL SSA. The partnership includes, on the Southeast Asian side, the national Agencies managing ICT research in the 7 most advanced countries, which, added to the fact that ASEAN has brought its support to the project, will ensure the largest participation of the Southeast Asian ICT community in project activities, and will provide a suited environment for the development of EU-Southeast Asia policy dialogues. The SEACOOP project is formally supported, on the European side, by leading organisations, including the main ETPs, which will ensure the highest involvement of, and the highest project impact on, the European ICT community.


Grant
Agency: Cordis | Branch: FP7 | Program: CSA | Phase: ICT-2009.9.1 | Award Amount: 954.50K | Year: 2010

The SEALING project, targeting the objective 9.1a of the FP7 ICT work programme, aims at supporting the development of Information Society policy dialogues and the strengthening of cooperation on ICT research with the ASEAN region in general (a region of strategic importance for the EU), and with Singapore in particular (a country with which a MoU on ICT has been signed).\n\nIt builds on the results achieved and the experience gained through the FP7 SEACOOP project, which has already supported, over the period 2007-2009, the development of S&T cooperation in ICT between the two regions.\n\nOn the Southeast Asian side, the SEALING partnership includes the organisation managing ICT research in each of the ten ASEAN countries and is formally supported by the ASEAN Secretariat.\n\nOn the European side, the project involves, through a Project Advisory Committee, over 20 leading companies, research institutes and academia. The project is also supported by the main European Technology Platforms and Joint Technology Initiatives in the ICT area, and by key European initiatives in Southeast Asia, namely the EU-SEA-NET INCO-Net project and the TEIN3 initiative.\n\nThe SEALING project will develop its activities over a 2-year period:\n Organization of at least 6 cooperation events in Europe and in Southeast Asia to be synchronised with policy dialogue meetings,\n Identification and analysis of ICT policy and research priorities, at bilateral and regional levels, and provision of recommendations for future cooperation initiatives,\n Development of synergies with dialogues and activities launched under EU-funded programmes (EU-SEA-NET, TEIN3, Marie Curie, etc.), at the initiative of EU Member States and Associated States, and at the ASEAN level (SCMIT),\n Development of promotion, dissemination and awareness-raising activities.

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