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Mincea M.,West University of Timisoara | Ionascu C.,West University of Timisoara | Kis K.,Advanced Environmental Research Laboratories | Kis K.,West University of Timisoara | And 2 more authors.
Studia Universitatis Babes-Bolyai Chemia | Year: 2016

A rapid, accurate and economic procedure has been applied to extract and analyze trace level of ochratoxin A (OTA) in instant coffee. The OTA was extracted and cleaned-up by methanol extraction followed by retention on IRA-400 anion exchanger and quantified by Ultra Performance Liquid Chromatography (UPLC) with MS/MS detection. Under optimum conditions, the detection limit (S/N = 3) was 0.05 ng/g and recoveries of OTA at spiking levels of 5, 10, and 20 ng/mL, were 82.67 ± 8.05%, 96.87 ± 4.63%, and 98.25 ± 4.46% respectively. © 2016, Universitatea Babes-Bolyai, Catedra de Filosofie Sistematica. All rights reserved. Source

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