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Zheng B.,Anhui University of Science and Technology | Zheng B.,Advanced Laboratory of Environmental Research and Technology ALERT | Cheng S.,Anhui University of Science and Technology | Cheng S.,Advanced Laboratory of Environmental Research and Technology ALERT | And 6 more authors.
Analytical Biochemistry | Year: 2013

Abstract Small molecules are difficult to detect by the conventional quartz crystal microbalance with dissipation (QCM-D) technique directly because the changes in frequency resulting from the binding processes of small biomolecules are often small. In the current study, an aptamer-based gold nanoparticles (AuNPs)-enhanced sensing strategy for detection of small molecules was developed. The QCM crystal was first modified with a layer of thiolated linker DNA, which can be partly base-paired with the detection part containing the adenosine aptamer sequence. In the presence of adenosine, the aptamer bound with adenosine and folded to the complex structure, which precluded the reporter part carrying AuNPs to combine with the random coiled detection part. Therefore, the lower the concentration of adenosine, the more AuNPs combined to the crystal. The resulting aptasensor showed a linear response to the increase of the adenosine concentration in the range of 0-2 μM with a linear correlation of r = 0.99148 and a detection limit of 65 nM. Moreover, the aptasensor exhibited several excellent characteristics such as high sensitivity, selectivity, good stability, and reproducibility.© 2013 Elsevier Inc. All rights reserved. Source


Feng T.,Hefei University of Technology | Feng T.,CAS Changchun Institute of Applied Chemistry | Feng T.,City University of Hong Kong | Feng T.,Advanced Laboratory of Environmental Research and Technology ALERT | And 9 more authors.
European Journal of Pharmaceutics and Biopharmaceutics | Year: 2014

PLGA porous microspheres loaded with doxorubicin (DOX) and paclitaxel (PTX) were developed for in situ treatment of metastatic lung cancer. The synergistic effect of the combined drugs was investigated against B16F10 cells to obtain the optimal prescription for in vivo studies. The combination therapy showed great synergism when DOX was the majority in the combination therapy, while they showed moderate antagonism when PTX is in major. The combination of DOX and PTX at a molar ratio of 5/1 showed the best synergistic therapeutic effect in the free form. However, the drugs exhibited more synergism in the PLGA microspheres at a molar ratio of 2/1, due to the difference in drug release rate. The in vivo study verified the synergism of DOX and PTX at the optimal molar ratio. These results suggested that dual encapsulation of DOX and PTX in porous PLGA microspheres would be a promising technology for long effective lung cancer treatment. © 2014 Elsevier B.V. All rights reserved. Source


Feng T.,CAS Changchun Institute of Applied Chemistry | Feng T.,City University of Hong Kong | Feng T.,Hefei University of Technology | Feng T.,Advanced Laboratory of Environmental Research and Technology ALERT | And 8 more authors.
Journal of Materials Chemistry B | Year: 2014

Two PEGylated poly(aspartate-g-OEI) cationic copolymers, PEG-b-pAsp-g-OEI (DAO) and OEI-g-pAsp-b-PEG-b-pAsp-g-OEI (TAO), were developed for in vivo gene transfer, and a non-PEGylated copolymer (MAO) was utilized as a control. These copolymers exhibited favorable capacities for condensing plasmid DNA (pDNA) into nanosized particles (90-180 nm) with positive surface charges. Gene transfection efficiency of the copolymers (especially DAO) demonstrated improved performance compared to PEI25k in both HeLa and HEK 293 cell lines in the presence of serum. Although MAO and DAO show similar gene transfection efficiency in vitro, DAO is shown to be more effective in vivo. The potential reason is that PEGylation enhances the serum-resistance of the carriers and prolongs gene transfection in vivo. For TAO, despite its PEG segment, the complex of copolymer-pDNA is encompassed by a cation shell and cannot reduce the serum effects. These results suggest that PEGylated diblock copolymers have potential as non-viral gene carriers in gene delivery systems for in vivo application. This journal is © the Partner Organisations 2014. Source


Qiu Y.,City University of Hong Kong | Wang L.,City University of Hong Kong | Wang L.,Advanced Laboratory of Environmental Research and Technology ALERT | Wang L.,Anhui University of Science and Technology | And 7 more authors.
Applied Catalysis A: General | Year: 2011

Nitrogen doped K2Nb4O11 (K 2Nb4O11-N) has been prepared by solid state reaction between K2Nb4O11 and urea at 400 °C. K2Nb4O11-N has been characterized by XRD, SEM, XPS and UV/vis diffuse reflectance. The photodegradation of various organic pollutants in water by this material, including Orange G (OG), bisphenol A (BPA) and pentachlorophenol (PCP) have been studied at λ > 330 nm and >399 nm. The results show that the photocatalytic activity of K 2Nb4O11-N at >399 nm is higher than those of K2Nb4O11 and Degussa TiO2 P25, indicating the activating effect of nitrogen doping. A mechanism for the photodegradation of organic substrates by K2Nb4O 11-N is proposed. © 2011 Elsevier B.V. All rights reserved. Source


Wang R.,Advanced Laboratory of Environmental Research and Technology ALERT | Wang R.,City University of Hong Kong | Wang R.,Anhui University of Science and Technology | Zhu Y.,City University of Hong Kong | And 4 more authors.
Applied Catalysis A: General | Year: 2013

A new silver tantalate material has been synthezied by heating a mixture of AgNO3, Ta2O5 and KCl at 850 C for 20 h. XRD, EDX, XPS, SEM, TEM and HRTEM show that the material consists of Ag 1.4K0.6Ta4O11 nanoplates, with Ag nanoparticles present on the surface, which is consistent with enhanced absorption of the material in the visible region. Addition of 0.5-5 mol% of La2O3 in the preparation of Ag1.4K 0.6Ta4O11 results in significant change in the morphology from nanoplates to nanoplates/nanowire composites. The photocatalytic activities of Ag1.4K0.6Ta4O11 and La-Ag1.4K0.6Ta4O11 have been evaluated by degradation of the organic pollutants rhodamine B (RhB) and pentachlorophenol (PCP) in water under visible light (λ > 420 nm), as well as by photocatalytic reduction of water to H2 at λ > 390 nm. The photocatalytic activity of Ag1.4K0.6Ta 4O11 is significantly enhanced by La-doping; the optimal La content is 1 mol% for degradation of organic substrates and 5 mol% for H 2 evolution, with photocatalytic activity significantly higher than that of P25 TiO2. The enhancement of photocatalytic activity upon La doping is attributed to trapping of excited electrons by La3+, and the formation of nanowires which further promote charge separation. © 2013 Elsevier B.V. All rights reserved. Source

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