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Huang W.-C.,National Chiao Tung University | Liu K.-H.,Advanced Delivery Technology Co. | Liu T.-C.,National Chiao Tung University | Liu D.-M.,National Chiao Tung University | Chen S.-Y.,National Chiao Tung University
Acta Biomaterialia | Year: 2014

In this study, a new type of polydimethylsiloxane-modified chitosan (PMSC) amphiphilic hydrogel was developed as a soft substrate to explore cellular responses for dermal reconstruction. The hydrogel wettability, mechanical stiffness and topography were controllable through manipulation of the degree of esterification (DE) between hydrophobic polydimethylsiloxane (PDMS) and hydrophilic N,O-(carboxymethyl)-chitosan (NOCC). Based on microphase separation, the incorporation of PDMS into NOCC increased the stiffness of the hybrid through the formation of self-assembled aggregates, which also provided anchor sites for cell adhesion. As the DE exceeded 0.39, the size of the PDMS-rich aggregates changed from nanoscale to microscale. Subsequently, the hierarchical architecture resulted in an increase in the tensile modulus of the hybrid gel up to fourfold, which simultaneously provided mechano-topographic guidance and allowed the cells to completely spread to form spindle shapes instead of forming a spherical morphology, as on NOCC (DE = 0). The results revealed that the incorporation of hydrophobic PDMS not only impeded acidic damage resulting from NOCC but also acted as an adhesion modification agent to facilitate long-term cell adhesion and proliferation on the soft substrate. As proved by the promotion on long-term type-I collagen production, the PMSC hybrid with self-assembled mechano-topography offers great promise as an advanced scaffold material for use in healing applications. © 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. Source


Chao C.-S.,National Chiao Tung University | Liu K.-H.,Advanced Delivery Technology Co. | Tung W.-L.,National Chiao Tung University | Chen S.-Y.,National Chiao Tung University | And 3 more authors.
Microporous and Mesoporous Materials | Year: 2012

Ultrathin mesoporous TiO 2 coatings with a wormhole-like architecture were synthesized using evaporation-induced self-assembly method. The morphological and chemical structures of TiO 2 films were characterized using small-angle and large-angle X-ray diffraction, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and Fourier transformed infrared spectrometry (FTIR). The films demonstrated a thickness of ∼120 nm and open-porous nanostructure. By taking advantage of the tortuosity of the worm-like mesoporous architecture associated with the chemistry of the TiO 2 film, a sustained drug release using ibuprofen and vancomycin as model molecules from the film was determined. Besides, adhesion behavior of osteoblast cells, together with an in vitro apaptitic formation substantiated the cytocompatibility and bioactivity of the mesoporous TiO 2 films. Such combined bioactive and drug-releasing functions of the TiO 2 films with worm-like mesoporosity ensure an improved therapeutic performance for potential applications included orthopedics, dentistry, and drug delivery. © 2011 Elsevier Inc. All rights reserved. Source


Lee S.-M.,Nursing and Management College | Liu K.-H.,Advanced Delivery Technology Co. | Liu Y.-Y.,Advanced Delivery Technology Co. | Chang Y.-P.,Advanced Delivery Technology Co. | And 2 more authors.
Materials | Year: 2013

Chitosonic® Acid, carboxymethyl hexanoyl chitosan, is a novel chitosan material that has recently been accepted by the Personal Care Products Council as a new cosmetic ingredient with the INCI (International Nomenclature of Cosmetic Ingredients) name Carboxymethyl Caprooyl Chitosan. In this study, we analyze several important cosmetic characteristics of Chitosonic® Acid. Our results demonstrate that Chitosonic® Acid is a water-soluble chitosan derivative with a high HLB value. Chitosonic® Acid can form a nano-network structure when its concentration is higher than 0.5% and can self-assemble into a nanosphere structure when its concentration is lower than 0.2%. Chitosonic® Acid has potent antimicrobial activities against gram-positive bacteria, gram-negative bacteria and fungus. Chitosonic® Acid also has moderate DPPH radical scavenging activity. Additionally, Chitosonic® Acid exhibits good hydration activity for absorbing and retaining water molecules with its hydrophilic groups. From a safety point of view, Chitosonic® Acid has no cytotoxicity to L-929 cells if its concentration is less than 0.5%. Moreover, Chitosonic® Acid has good compatibilities with various normal cosmetic ingredients. Therefore, we propose that Chitosonic® Acid has the potential to be a widely used ingredient in various types of cosmetic products. © 2013 by the authors. Source


Huang L.-Y.,National Taiwan University of Science and Technology | Liu T.-Y.,National Taiwan University | Liu K.-H.,Advanced Delivery Technology Co. | Liu Y.-Y.,Advanced Delivery Technology Co. | And 3 more authors.
Journal of Nanoscience and Nanotechnology | Year: 2012

Novel amphipathic derivative of chitosan (carboxymethyl-hexanoyl chitosan. CHC) was made into mats of nanofibers (?100 nm) via electrospinning. The resulting mats were further cross-linked with genipin. The morphology of CHC nanofibers was examined using a field emission scanning electron microscope (FESEM). The optimum parameters of CHC nanofiber was achieved when the CHC concentration was 4 wt% and electrospinning was conducted with a voltage of 20 kV over a distance of 10 cm. The characterizations of biocompatibility, hemocompatibility, and anti-bacterial activity of the nanofibers were also investigated. The results show that CHC nanofibers still preserved antibacterial activity and thrombogeneicity owing to those residual amino groups of chitosan and exhibit high biocompatibility for L929 fibroblast test. Thus CHC exhibited the potential to serve as a novel wound dressing and surgical implants application by these advanced features. Copyright © 2012 American Scientific Publishers All rights reserved. Source

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