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PubMed | University of the Republic of Uruguay, Federal University of Rio de Janeiro, University of Santiago de Chile, Institute Investigaciones Marinas and 11 more.
Type: Journal Article | Journal: Genome announcements | Year: 2014

Chuil Lagoon in central Chile harbors distinct microbial communities in various solar salterns that are arranged as interconnected ponds with increasing salt concentrations. Here, we report the metagenome of the 3.0- to 0.2-m fraction of the microbial community present in a crystallizer pond with 34% salinity.

Lomas M.W.,Bermuda Institute of Ocean Sciences | Bronk D.A.,Virginia Institute of Marine Science | Van Den Engh G.,BD Advanced Cytometry Group
Annual Review of Marine Science | Year: 2011

An important goal of marine biogeochemists is to quantify the rates at which elements cycle through the ocean's diverse microbial assemblage, as well as to determine how these rates vary in time and space. The traditional view that phytoplankton are producers and bacteria are consumers has been found to be overly simplistic, and environmental metagenomics is discovering new and important microbial metabolisms at an accelerating rate. Many nutritional strategies previously attributed to one microorganism or functional group are also or instead carried out by other groups. To tease apart which organism is doing what will require new analytical approaches. Flow cytometry, when combined with other techniques, has great potential for expanding our understanding of microbial interactions because groups can be distinguished optically, sorted, and then collected for subsequent analyses. Herein, we review the advances in our understanding of marine biogeochemistry that have arisen from the use of flow cytometry. Copyright © 2011 by Annual Reviews. All rights reserved.

Von Dassow P.,University of Santiago de Chile | Van Den Engh G.,BD Advanced Cytometry Group | Iglesias-Rodriguez D.,UK National Oceanography Center | Gittins J.R.,UK National Oceanography Center
Journal of Plankton Research | Year: 2012

Coccolithophores are important primary producers and a dominant group of calcifying organisms in the ocean. Calcification state depends on genetic, physiological and environmental factors. We show that flow cytometric measurement of the depolarization forward scattered light using a Brewster's Window Analyzer can be used to quantify the degree of calcification of coccolithophores at the single-cell level. Calcite-containing particles or cells were distinguished from non-calcified particles or cells by high values of forward scatter light with polarization orthogonal to that of the laser. Forward scatter polarization state varied strongly and linearly with the number of attached coccoliths per coccosphere when Emiliania huxleyi cells were first completely decalcified and then allowed to rebuild coccospheres. Cells of the heavily calcified E. huxleyi R-morphotype strain NZEH were also grown in different extracellular Ca2 concentrations, forming complete coccospheres that contained similar numbers of attached coccoliths but varied in total calcite mass. Forward scatter polarization state varied strongly and linearly with coccosphere calcite mass. In contrast, forward scatter polarization state of detached coccoliths did not vary significantly with calcite weight, although forward scatter and side scatter did. Treatments had relatively minor effects on forward scatter, side scatter and forward scatter polarization state of decalcified cells, suggesting that depolarization of forward scatter light from E. huxleyi cells might be linearly determined, to a first approximation, by the ratio of surface calcite to organic protoplast. We suggest that flow cytometric measurement of forward scatter depolarization provides a potentially valuable method for analysis of calcification state of individual cells. © 2012 The Author.

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