Time filter

Source Type

Ōita-shi, Japan

Arai K.,Waseda University | Ogata M.,Waseda University | Fujiwara M.,Waseda University | Tsutsumi H.,Showa Womens University | And 4 more authors.
Indoor Air 2014 - 13th International Conference on Indoor Air Quality and Climate | Year: 2014

We propose an infection-control bed as an infection-control technique using a push-pull local ventilation system and evaluated this bed in this study. The effectiveness in reducing the risk of airborne infection was evaluated using a cough-simulation machine. The machine releases carbon dioxide (CO2) as a tracer gas. The simulated cough was set up to be released in two directions. The directions were assumed such that they typically pose an infection risk to the doctor. In addition, the diffusion-prevention effect in the hospital room was also studied. When the cough was released directly above the bed, the amount of CO2 gas sampled at the measurement points above the patient decreased. The concentration at the most effective measurement point was 91.1 % lower as compared to that in the control case. When the cough was released directly in the doctor's direction, the concentration at the doctor's respiratory area was 56.7 % lower as compared to that in the control case.

A method for detecting or quantifying an analyte, using a test strip for lateral flow type chromatography which contains a membrane and a detection part on which a capturing ligand that is to specifically bind to the analyte has been fixed on the membrane, includes: bringing an analyte contained in a sample into contact with a labeled ligand labeled with a phosphor that is to be excited by light having a wavelength from 600 nm to 800 nm to generate fluorescence, bringing a complex containing the analyte and the labeled ligand into contact with a capturing ligand at the detection part, and irradiating on the test strip light having a wavelength from 600 nm to 800 nm as an excitation light for the phosphor contained in the complex to generate fluorescence from the phosphor, and measuring a fluorescence intensity of the fluorescence.

Adtec Productions Incorporated and ADTEC Inc. | Date: 2005-02-01


Santivanez S.J.,Instituto Peruano Of Parasitologia Clinica Y Experimental | Rodriguez M.L.,Instituto Peruano Of Parasitologia Clinica Y Experimental | Rodriguez S.,Instituto Nacional Of Ciencias Neurologicas | Sako Y.,Asahikawa University | And 9 more authors.
Journal of Clinical Microbiology | Year: 2015

Diagnosis of cystic echinococcosis (CE) is based on the identification of the cyst(s) by imaging, using immunodiagnostic tests mainly as complementary tools in clinical settings. Among the antigens used for immunodiagnosis, previous studies described a good performance of the recombinant antigen B8/1 (rAgB) in an enzyme-linked immunosorbent assay (ELISA) format; however, in remote parts of areas where the disease is endemic, the implementation of an ELISA is difficult, so a more simple, rapid, and reliable method such as the immunochromatographic test (ICT) is required. In this study, using a set of 50 serum samples from patients with surgically confirmed CE, we compared the performance of an ICT and that of an ELISA using the rAgB. The overall sensitivities of ICT and ELISA were not statistically different (78% versus 72%; P=0.36). The overall agreement between both tests was moderate (κ=0.41; P<0.01). Concordance between ICT and ELISA was substantial or almost perfect for patients with liver involvement (κ=0.65; P<0.001) and patients with more than one hydatid cyst (κ=0.82; P<0.001), respectively. Moreover, specificity analysis using a total of 88 serum samples from healthy individuals (n=20) and patients (n=68) with other parasitic infections revealed that ICT had a specificity of 89.8%. ICT and ELISA had similar performance for the detection of specific antibodies to E. granulosus, and ICT had a high specificity, opening the possibility of using ICT as a screening tool in rural settings. © 2015, American Society for Microbiology.

Nakano S.,Tokyo Metropolitan Institute of Medical Science | Nakano S.,Synthera Technologies Co. | Tsukimura T.,Meiji Pharmaceutical University | Togawa T.,Meiji Pharmaceutical University | And 10 more authors.
PLoS ONE | Year: 2015

We developed an immunochromatography-based assay for detecting antibodies against recombinant α-galactosidase A proteins in serum. The evaluation of 29 serum samples from Fabry patients, who had received enzyme replacement therapy with agalsidase alpha and/ or agalsidase beta, was performed by means of this assay method, and the results clearly revealed that the patients exhibited the same level of antibodies against both agalsidase alpha and agalsidase beta, regardless of the species of recombinant α-galactosidase A used for enzyme replacement therapy. A conventional enzyme-linked immunosorbent assay supported the results. Considering these, enzyme replacement therapy with agalsidase alpha or agalsidase beta would generate antibodies against the common epitopes in both agalsidase alpha and agalsidase beta. Most of the patients who showed immunopositive reaction exhibited classic Fabry phenotype and harbored gene mutations affecting biosynthesis of α-galactosidase A. As immunochromatography is a handy and simple assay system which can be available at bedside, this assay method would be extremely useful for quick evaluation or first screening of serum antibodies against agalsidase alpha or agalsidase beta in Fabry disease with enzyme replacement therapy. Copyright: © 2015 Nakano et al.

Discover hidden collaborations