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Kumar H.,University of Agricultural Sciences, Bangalore | Agasthya A.S.,Administrative Management College | Rijesh K.,University of Agricultural Sciences, Bangalore | Murugesh A.,University of Agricultural Sciences, Bangalore | And 2 more authors.
Asian Journal of Microbiology, Biotechnology and Environmental Sciences | Year: 2010

Saccharomyces cerevisiae gained particular attention in the 1990's as it has served as an important scientific model and tool, and was the first eukaryotic organism to have its genome sequenced. Mutations may be induced by exposure to ultraviolet rays and alpha, beta, gamma, and X radiation, by extreme changes in temperature, and by certain mutagenic chemicals such as nitrous acid, nitrogen mustard, and chemical substitutes for portions of the nucleotide subunits of genes. Ethidium bromide ("EtBr"), is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis. Ethidium bromide may be a strong mutagen. The molecular markers are one of the versatile tools in Molecular Biology and Genetic Engineering. Molecular genetic techniques can be used to discriminate between yeast strains that have similar physiological characteristics. In the present study, induced mutation was carried out with interchelating agent like ethidium bromide with four different concentrations, 0.25, 0.50, 0.75 and 1 μg/ml. Random Amplified Polymorphic DNA (RAPD) analysis was employed to characterize yeast isolates. Five Saccharomyces strains were subjected to RAPD analysis using nine primers, selected from the kits of Chromous Biotech. Dendrogram was constructed according to Ward's method based on the scorable bands using Statistica software. Cluster analysis of dendrograms revealed that all the five yeast isolates formed two major clusters. © Global Science Publications. Source


Leelaprakash G.,Administrative Management College
Research Journal of Chemistry and Environment | Year: 2010

The purpose of this study was to investigate the anticancer of alcohol (95%) extract of the whole plant of Phyllanthus rheedii. The MTT (methylthiazolyldiphenyl-tetrezolinum bromide) method was applied to compare the antitumoral activity of alcohol (95%) extract on lung carcinoma cell lines (A549), colon carcinoma cell lines (HCT-116), liver carcinoma cell lines (HEPG-2) and cervical carcinoma cell lines (HELA). Source


Kumar H.,Bangalore University | Agasthya A.S.,Administrative Management College | Rijesh K.,University of Agricultural Sciences, Bangalore | Murugesh A.,Sathyabhama College | And 2 more authors.
Asian Journal of Microbiology, Biotechnology and Environmental Sciences | Year: 2010

The investigation was carried out to isolate yeast strains from their natural habitats and to screen them for ethanol tolerance. A total of 5 yeast strains were isolated from various sources like Grape, Apple, Distillery effluents, Standard yeast and Dry yeast cultured on Yeast Extract Potato Dextrose Agar media. All of these five samples were used to isolate five strains of yeast identified as Saccharomyces strains based on colony type and cell morphology and budding characters. Saccharomyces species were screened for the ability to tolerate different ethanol concentrations from 8-14%. Growth in different ethanol concentrations varied from one strain to another. Mutation was induced with intercalating agent like Acridine Orange with four different concentrations, (0.25, 0.50. 0.75 and 1 mg/mL). Yeast strains showed tolerance level from 8-14%. Mutated strain YMSY showed highest tolerance to ethanol stress and YMDY with lowest tolerance. Mutants showed decreased growth and tolerance under high ethanol stress compared to their original isolates. © Global Science Publications. Source


Hemapriya J.,Administrative Management College | Kannan R.,Bharathidasan University | Vijayanand S.,Administrative Management College
Journal of Pure and Applied Microbiology | Year: 2010

Bacterial strains (DRS-1, DRS-2,DRS-3,DRS-4) isolated from dye contaminated sludge,decolorized an azo dye namely Direct Red-28.Of these,DRS-1 & DRS-3 were selected for further studies because of their high efficiency to decolorize the dye. Effect of Physio-chemical parameters, such as aeration/agitation, concentration of glucose, peptone and PH of the culture medium on dye decolourization by isolates DRS-1 & DRS-3 were analyzed.DRS-1 had higher dye decolorizing ability than DRS-3.Under optimal conditions, DRS-1 decolorized Direct Red-28 (100ppm) upto 94.80%.The highest decolourization ability of isolate DRS-1 can thus be exploited in the treatment of industrial effluent containing azo dyes. Source

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