Aditya Institute of Pharmaceutical science and Research

andhra Pradesh, India

Aditya Institute of Pharmaceutical science and Research

andhra Pradesh, India
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Phani Kumar C.,Aditya Institute of Pharmaceutical science and Research
Drug Invention Today | Year: 2013

Hepatotoxicity in one of the major parameters need to be consider in drug therapy, because most of the drugs in single at low/high doses for a prolonged period or multiple drug therapy causes liver damage. Chicory is a Mediterranean herb that has been used as a coffee filler or substitute. It contains no caffeine and has a chocolate-like flavor. Aim: The present study aimed to investigates the possible hepatoprotective activity of Cichorium intybus (Linn.) against carbon tetrachloride induced hepatotoxicity in albino Wistar rats. Methods: Alcoholic and aqueous extracts of the C. intybus (Linn.) roots were used in the study for determination of hepatoprotective activity against carbon tetrachloride induced hepatotoxicity in albino Wistar rats. A comparison was also made between the actions of C.intybus (Linn.) root extracts and a known hepatoprotective drug silymarin. Preliminary phytochemical screening was carried. Biochemical parameters like SGOT, SGPT, SALP and total bilirubin and histopathological studies were used to evaluate the hepatoprotective activity. Acute toxicity studies were carried out according to OECD (420) guidelines and 400 mg/kg BW for both extracts was selected for the present study. Results: Showed that both extracts found to have significant hepatoprotective activity, reduces the elevated levels of SGOT, SGPT, SALP and Total bilirubin. But aqueous extract found to have hepatoprotective activity almost compared to silymarin. Histopathology showed regeneration of hepatocytes to normal, with the root extracts further confirmed the hepatoprotective activity of extracts of C. intybus (Linn.). Conclusion: Results indicate that it is worth undertaking further studies on possible usefulness of the extracts of the roots of C. intybus (Linn.) in hepatotoxicity. © 2013.


Killi G.D.,Sri Sai Aditya Institute of Pharmaceutical science and Research | Maddinapudi R.K.,Matrix | Dinakaran S.K.,Aditya Institute of Pharmaceutical science and Research | Harani A.,Andhra University
Brazilian Journal of Pharmaceutical Sciences | Year: 2014

A simple gradient Ultra Performance liquid chromatographic method (UPLC) was developed for determination of lopinavir and ritonavir from its related impurities and assay for the first time. This method involves the use of a C18 (Acquity UPLC BEH C18, 50 × 2.1 mm, 1.7 µm) column thermostated at 30 oC using triethylamine (pH 2.2): 0.1% H3PO4 in acetonitrile and methanol (85:15) as mobile phase in gradient elution mode. A Photo Diode Array (PDA) detector set at 215 nm was used for detection with flow rate 0.4 mL/min. This method was validated over the range of limit of quantitation (LOQ) to 50 to 150% of impurity specification limit and of working concentration for assay. The developed method was validated for linearity, range, precision, accuracy and specificity. This method was successfully applied for content determination of lopinavir and ritonavir in pharmaceutical formulations. This method can be conveniently used in quality control laboratory for routine analysis for assay and related substances as well as for evaluation of stability samples of bulk drugs and pharmaceutical formulations. © 2014, Faculdade de Ciencias Farmaceuticas (Biblioteca). All rights reserved.


Kumar O.,Drugs Control Administration | Rani A.P.,Acharya Nagarjuna University | Kumar D.V.,Aditya Institute of Pharmaceutical science and Research
Journal of Chemical and Pharmaceutical Research | Year: 2011

Flurbiprofen is a potent antii{dotless}inflammatory analgesic agent indicated for acute and chronic treatment of rheumatoid arthritis, osteoarthritis, and ankylosing spondylytis. Flurbiprofen is poorly water soluble and may show dissolution limited absorption. The Solid dispersions (SD) of Flurbiprofen by fusion method were prepared using 1:1, 1:4 and 1:5 ratios of drug and polymers (PEG 6000, PVP K30 and Urea). The Solid dispersions (SD) were characterized for physical appearance, solubility, FT-I.R. and in vitro dissolution studies. FT-IR study revealed that drug was stable in Solid dispersions (SD). Solubility of Flurbiprofen from Solid dispersions increased in distilled water. The drug content was found to be high and uniformly distributed in all formulations. The in vitro dissolution studies were carried using USP type XXVII (paddle) type dissolution apparatus. The prepared Solid dispersions showed marked increase in the dissolution rate of Flurbiprofen than that of pure Flurbiprofen drug. The dispersion with PEG 6000 (1:5) by fusion method showed faster dissolution rate (99.56%) as compared to other dispersions with PVP K30 and Urea (1:1, 1:4 and 1:5) whichever prepared by physical mixture (PM) and fusion method. Of the three carriers used, dissolution of the drug was more in PEG 6000 based Solid dispersions. It is concluded that dissolution of the Flurbiprofen could be improved by the Solid dispersion and PEG 6000 based solid dispersions were more effective in enhancing the dissolution.


Sridhar N.,P.A. College | Surya Kiran B.V.V.S.,P.A. College | Rudrapal M.,Aditya Institute of Pharmaceutical science and Research
Asian Journal of Pharmaceutical Research and Health Care | Year: 2014

Based on the ethnobotanical importance as diuretics Aerva lanata and Ecbolium ligustrinum were selected for present screening. Experiment was carried out on male wister strain albino rats using furosemide as standard drug. 20mg/kg and 200mg/kg oral doses are selected for standard and test respectively. For quantifying the diuretic activity the urine output, urine pH, sodium, potassium and chloride levels in the urine are measured. The diuretic index value of Ecbolium ligustrinum is 1.70 and nearer to the standard furosemide value 1.80. Aerva lanata group shows less diuretic index of 1.49 compared to standard. The ratio of the concentration of sodium ions to the potassium ions in control group was found to be 1.39 and the ratio for standard, Aerva lanata and Ecbolium ligustrinum are 1.78, 1.49 and 1.68 respectively. Ecbolium ligustrinum affected the amount of urine excreted and also the electrolyte concentration in urine. The present study concluded that roots of Aerva lanata and Ecbolium ligustrinum are having diuretic nature.


Rudrapal M.,Aditya Institute of Pharmaceutical science and Research
Indian Journal of Natural Products and Resources | Year: 2012

An ethnomedicinal survey was carried out in 10 different villages of East Godavari district of Andhra Pradesh with an aim to document the information regarding folkloric uses of indigenous plant species. A total of 32 plant species belonging to 32 genera and 25 families were recorded, and enumerated along with their botanical name, family, local name, part(s) used, ethnomedicinal uses including their method of preparation, mode of administration and dosage. The reported plant species in the form of various traditional preparations are employed by the village people for the management as well as treatment of various ailments ranging from snake bite to fever.


Palaksha M.N.,Aditya Institute of Pharmaceutical science and Research | Das S.,Aditya Institute of Pharmaceutical science and Research
Journal of Natural Science, Biology and Medicine | Year: 2010

This study focuses the significant antibacterial activity of Garlic (Allium sativum Linn.) extract on streptomycin-resistant strains solely and in synergism with streptomycin. Gram-positive Staphylococcus aureus ATCC BAA 1026 and gram-negative Escherichia coli ATCC 10536 were made resistant to standard antibiotic streptomycin used as a control in the experiment. Zones of inhibition of different treatment groups were measured by agar-well-diffusion assay and compared with control. Statistical comparison of sole extract and streptomycin synergism with streptomycin control had proved it significant.


Sarwa K.K.,Dibrugarh University | Mazumder B.,Dibrugarh University | Rudrapal M.,Dibrugarh University | Rudrapal M.,Aditya Institute of Pharmaceutical science and Research | Verma V.K.,Dibrugarh University
Drug Delivery | Year: 2015

In the present study, the biopotential of capsaicin (an active principle of capsicum) as a topical antiarthritic agent was studied in arthritic rats. Transfersomal vesicular system was employed for the topical administration of capsaicin in experimental rats. The characterization of prepared capsaicin-loaded transfersomes reveals their nano size (94 nm) with negative surface charge (-14.5 mV) and sufficient structural flexibility, which resulted in 60.34% entrapment efficacy, penetration across the biomembrane (220 μm) and 76.76% of drug release from vesicular system in 24 h in their intact form as evident from confocal laser scanning micrographic study. Results of transfersomal nanoformulation (capsaicin loaded, test) were compared with that of conventional gel formulation available in the market (Thermagel, standard), with an aim to assess the antiarthritic efficacy of our prepared capsaicin-loaded transfersomal formulation. In vivo antiarthritic activity study shows that our formulation possesses superior inhibitory activity than the marketed Thermagel formulation at the same dosage level, which could probably be due to the lesser permeability of Thermagel across the dermal barriers compared to our specially designed transfersomal delivery system. Moreover, the better tolerance of prepared vesicular formulation in biological system further enlightens the suitability of the transfersomal vesicle to be used as a novel carrier system for the topical administration of such highly irritant substance. © 2014 Informa Healthcare USA, Inc. All rights reserved.


Kumar Sarwa K.,Dibrugarh University | Rudrapal M.,Dibrugarh University | Rudrapal M.,Aditya Institute of Pharmaceutical science and Research | Mazumder B.,Dibrugarh University
Drug Delivery | Year: 2015

In this study, topical ethosomal formulation of capsaicin was prepared and evaluated for bio-efficacy in arthritic rats. Physical and biological characterizations of prepared capsaicin-loaded nano vesicular systems were also carried out. Ethosomal capsaicin showed significant reduction of rat paw edema along with promising antinociceptive action. The topical antiarthritic efficacy of prepared formulation of capsaicin was found more than that of Thermagel, a marketed gel of capsaicin. From toxicological study, no predictable signs of toxicity such as skin irritation (of experimental rats) were observed. Based on this finding, ethosomal capsaicin could be proposed as an effective as well as a safe topical delivery system for the long-term treatment of arthritis and associated inflammo-musculoskeletal disorders. Such exciting result would eventually enlighten the analgesic and anti-inflammatory potential of capsaicin for topical remedy. © 2014 Informa Healthcare USA, Inc.


Rudrapal M.,Aditya Institute of Pharmaceutical science and Research | Chetia D.,Dibrugarh University
Pharma Times | Year: 2014

Cancer is a serious health problem affecting people of all ages around the world. Today, the high incidence risk of cancer is mainly attributed to globalization of unhealthy lifestyles; particularly cigarette smoking, inadequate diet like reduced intake of certain edible fruits and vegetables, adopting western modern diet rich in high fat and low fiber content and presence of certain carcinogenic chemicals in foods etc. Recent research has revealed that regular consumption of certain fruits and vegetables can reduce the risk of developing malignant cancers. In this context, dietary phytochemicals (non-nutritive components in the plant-based diet) are considered as potential alternative sources of safer chemicals which possess cancer preventive properties. Such dietary phytochemicals have been known to possess at various degrees their individual chemo-preventive potential against different malignant forms of cancers. These are capsaicin (chili pepper), curcumin (turmeric), diallylsulfide (garlic), epigallocatechin-3-gallate (green tea), genistein (soyabeans), 6-gingerol (ginger), indole-3-carbinol (cabbage), resveratrol (grapes); just to name a few. The chemoprevention of cancer by these edible phytochemicals remains to be a cost-effective, readily accessible and therapeutically effective approach for the better management of cancers in general public. © 2014, Indian Pharmaceutical Association. All rights reserved.


Rao R.N.,Indian Institute of Chemical Technology | Prasad K.G.,Indian Institute of Chemical Technology | Kumar K.V.S.,Aditya Institute of Pharmaceutical science and Research | Ramesh B.,Indian Institute of Chemical Technology
Analytical Methods | Year: 2013

A rapid, sensitive and selective bio-analytical method was developed for determination of elvitegravir and ritonavir in rat plasma by high-performance liquid chromatography-tandem mass spectrometry. Sample preparation was based on diatomaceous earth supported liquid extraction using dichloromethane to extract elvitegravir and ritonavir from rat plasma. Chromatographic separation was performed on a Waters Symmetry C18 column (4.6 × 250 mm, 5 μm) using 20 mM ammonium formate containing 0.05% trifluoroacetic acid and acetonitrile (35:65 v/v) for isocratic elution and mass detection. Calibration curves were linear with regression coefficients greater than 0.997 over the concentration range of 2-5000 ng mL-1 for elvitegravir and ritonavir. Absolute mean recoveries were in the range of 87.31 to 92.18% and no significant interferences were observed at the retention times of the elvitegravir, ritonavir and internal standard. The method exhibited good intra- and inter-day performance in terms of 1.55-7.47% precision and 0-5% accuracy. The developed method has been successfully applied to the pharmacokinetic study of elvitegravir in healthy rats following a single oral administration of a 15 mg kg-1 dose boosted with 5 mg kg-1 ritonavir. © 2013 The Royal Society of Chemistry.

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