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Rudrapal M.,Aditya Institute of Pharmaceutical science and Research
Indian Journal of Natural Products and Resources

An ethnomedicinal survey was carried out in 10 different villages of East Godavari district of Andhra Pradesh with an aim to document the information regarding folkloric uses of indigenous plant species. A total of 32 plant species belonging to 32 genera and 25 families were recorded, and enumerated along with their botanical name, family, local name, part(s) used, ethnomedicinal uses including their method of preparation, mode of administration and dosage. The reported plant species in the form of various traditional preparations are employed by the village people for the management as well as treatment of various ailments ranging from snake bite to fever. Source

Rao R.N.,Indian Institute of Chemical Technology | Prasad K.G.,Indian Institute of Chemical Technology | Kumar K.V.S.,Aditya Institute of Pharmaceutical science and Research | Ramesh B.,Indian Institute of Chemical Technology
Analytical Methods

A rapid, sensitive and selective bio-analytical method was developed for determination of elvitegravir and ritonavir in rat plasma by high-performance liquid chromatography-tandem mass spectrometry. Sample preparation was based on diatomaceous earth supported liquid extraction using dichloromethane to extract elvitegravir and ritonavir from rat plasma. Chromatographic separation was performed on a Waters Symmetry C18 column (4.6 × 250 mm, 5 μm) using 20 mM ammonium formate containing 0.05% trifluoroacetic acid and acetonitrile (35:65 v/v) for isocratic elution and mass detection. Calibration curves were linear with regression coefficients greater than 0.997 over the concentration range of 2-5000 ng mL-1 for elvitegravir and ritonavir. Absolute mean recoveries were in the range of 87.31 to 92.18% and no significant interferences were observed at the retention times of the elvitegravir, ritonavir and internal standard. The method exhibited good intra- and inter-day performance in terms of 1.55-7.47% precision and 0-5% accuracy. The developed method has been successfully applied to the pharmacokinetic study of elvitegravir in healthy rats following a single oral administration of a 15 mg kg-1 dose boosted with 5 mg kg-1 ritonavir. © 2013 The Royal Society of Chemistry. Source

Rudrapal M.,Aditya Institute of Pharmaceutical science and Research
Asian Journal of Chemistry

A RP-HPLC method was developed and validated for simultaneous determination of minoxidil and aminexil in a combined topical liquid formulation. Isocratic separation was achieved on a C18 column (250 mm × 4.6 mm i.d., particle size 5 μm, ThermoHypersil BDS) using a mobile phase consisting of methanol-phosphate buffer (pH 3.0, 60:40, v/v) at a flow rate of 1 mL/min and UV detection at 250 nm. Linearity was observed over the concentration range of 1.0-2.0 mg/mL for both minoxidil (r2 = 0.99) and aminexil (r2 = 0.99). The average percentage recovery of the method was 101.0 % with a relative standard deviation of 0.14 %. The limits of detection (LODs) were 0.7346 μg/mL and 0.2423 μg/mL for minoxidil and aminexil and limits of quantification (LOQs) were 0.7346 μg/mL and 0.2423 μg/mL, respectively. The method was validated as per ICH guidelines. Source

Palaksha M.N.,Aditya Institute of Pharmaceutical science and Research | Das S.,Aditya Institute of Pharmaceutical science and Research
Journal of Natural Science, Biology and Medicine

This study focuses the significant antibacterial activity of Garlic (Allium sativum Linn.) extract on streptomycin-resistant strains solely and in synergism with streptomycin. Gram-positive Staphylococcus aureus ATCC BAA 1026 and gram-negative Escherichia coli ATCC 10536 were made resistant to standard antibiotic streptomycin used as a control in the experiment. Zones of inhibition of different treatment groups were measured by agar-well-diffusion assay and compared with control. Statistical comparison of sole extract and streptomycin synergism with streptomycin control had proved it significant. Source

Dinakaran S.K.,Aditya Institute of Pharmaceutical science and Research | Dinakaran S.K.,Jawaharlal Nehru University | Avasarala H.,Aditya Institute of Pharmaceutical science and Research
Journal of Dietary Supplements

The present study involves the determination of antioxidant capacity and in vitro α-amylase and lipase inhibitory activity of the Crotalaria juncea Linn extract. The content of polyphenols, flavonoids, and tannins in the extracts was estimated by spectrophotometry. Antioxidant activity on goat liver lipid peroxidation and linoleic acid emulsion were determined and α-amylase and lipase inhibitory activity was also evaluated. All the extracts had shown antioxidant property, α-amylase, and lipase inhibitory properties. Aqueous extract was found to show maximum antioxidant activity on goat liver. Antilipid peroxidation and antioxidant activity were determined to be 66.94 ± 0.616 (p < .01) and 59.54 ± 0.2 (p < .01), respectively. Maximum α-amylase and lipase inhibitory activities of 71.42 ± 1.37 (p < .01) and 57.14 ± 2.74% (p < .01), respectively, were exhibited by macerated methanol extract. The results had shown that all the extracts exhibited low inhibition and antioxidant activity as compared to standard. © 2014 Informa Healthcare USA, Inc. Source

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