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Yadu B.,Pandit Ravishankar Shukla University | Chandrakar V.,Pandit Ravishankar Shukla University | Meena R.K.,Aditya Biotech Laboratory and Research Pvt. Ltd. | Keshavkant S.,Pandit Ravishankar Shukla University
South African Journal of Botany | Year: 2017

Excess of fluoride (F) in irrigation water is a serious threat inducing variety of deleterious impacts in plants via over-production of active oxygen species (AOS), modifying antioxidant enzymes and nucleic acid. Present study was aimed to monitor protective functions of glycinebetaine (GB) against F-toxicity in Cajanus cajan L. GB is an osmolyte, serves as quencher of AOS and stabilizes membranes and proteins in stressed tissues. Seeds of C. cajan L. were exposed to F (75 ppm of NaF), and its combination with GB (50 μM), for five days. The results indicated that F caused inhibited growth, membrane stability index (MSI) and protein content, which were inversely related with the AOS levels and F sequestered. Moreover, few other stress markers viz.; malondialdehyde (MDA), 4-hydroxy-2-nonenal (4-HNE), lipoxygenase (LOX), and DNA polymorphism were found to be enhanced significantly, with a little of proline, in response to F. On the other hand, exogenous addition of GB exhibited improved growth, MSI, genomic template stability, protein and proline accumulations with lower levels of AOS, MDA, 4-HNE, LOX and DNA polymorphism in stressed tissue. Additionally, alterations in the activities/isoforms of superoxide dismutase, catalase, guaiacol peroxidase and ascorbate peroxidase were examined in F-stressed samples, and were found to be up-regulated by GB. This study concludes that GB counteracts F-toxicity strongly via restricting accumulation of F, AOS operated membrane deterioration and injury symptoms, and improving proline accumulation, defensive function and genomic template stability. Conducted study for the first time provides evidence that support the protective function of GB against F-toxicity. © 2017 SAAB

PubMed | Aditya Biotech Laboratory and Research Pvt. Ltd., Central Laboratory Facility and Pandit Ravishankar Shukla University
Type: | Journal: Plant physiology and biochemistry : PPB | Year: 2017

Presence of the toxic metalloid, arsenic (As) is ubiquitous in the environment especially in the soil and water. Its excess availability in the soil retards growth and metabolism of plants via (a) slowing down the cell division/elongation, (b) overproduction of reactive oxygen species (ROS), (c) modulation of antioxidant enzymes, and (d) alteration of DNA profile/genomic template stability (GTS). In the current study, diphenylene iodonium (DPI), 24-epibrassinolide (EBL) and proline (Pro) were used to analyze their roles in eliminating the adverse effects of As. Glycine max L. (variety JS 335) seeds were subjected to As (75M, Sodium arsenite was used as source of As), and in combination with DPI (10M), EBL (0.5M) or Pro (10mM), for five consecutive days, and effects of these treatment combinations were analyzed on germination percentage, biomass, membrane stability, GTS and expressions of defensive genes. In addition, the levels of As, ROS, malondialdehyde, DNA content, oxidation, fragmentation, polymorphism, DNase activity, endogenous Pro and pyrroline-5-carboxylate synthetase activity were evaluated. The results indicated that the treatments of DPI, EBL or Pro are capable to alleviate detrimental effects of As, gauged from above variables, but with different magnitudes. Apropos As-stress mitigation, Pro was found to be the most effective under the confines of the study protocol. This study certainly provides new ideas for intensifying studies to unravel elusive central mechanism of amelioration involving use of DPI, EBL or Pro in plants with confirmed As-toxicity.

PubMed | Aditya Biotech Laboratory and Research Pvt. Ltd.
Type: | Journal: Interdisciplinary sciences, computational life sciences | Year: 2016

In rice, ferric-chelate reductase-1 (FRO1) (LOC_Os04g36720) gene was present on chromosome number 4 and its beginning and ending coordinates where coding sequence lies are 22182599 and 22186943, respectively. It plays a vital role in metal homeostasis and iron transportation in plants. Based on the alignment results, location of single-nucleotide variants is located in open reading frame and their effects of variants were predicted using SIFT sequence tool. The non-synonymous variants at position 342 and 436 lies in helical and coil parts of the protein, respectively, as predicted by Psi-pred server. PSI-Blast which resulted in significant hits and the most similar protein sequence (Accession ID: NP_001052896) with available sequence features displayed 100% identity with query cover of 99%. Results suggest the non-synonymous variant at position 436 (Accession ID: TBGI204002) lies in FAD-binding domain and nsSNV at position 342 (Accession ID: TBGI203998) lies in periphery of NADP. The SNPs were also analyzed for the deleterious effect by PANTHER subPSEC scores and I-mutant score, and it was postulated that SNPs would be hampering on biological as well as molecular function of FRO 1 gene of rice. A cutoff of -3 corresponds to a 50% probability that a score is deleterious. From this, the probability that a given variant will cause a deleterious effect on protein function is estimated by P

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