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Marietta, GA, United States

Sarangapani K.K.,Georgia Institute of Technology | Sarangapani K.K.,University of Washington | Marshall B.T.,Georgia Institute of Technology | Marshall B.T.,Aderans Research Institute Inc. | And 2 more authors.
Journal of Biological Chemistry | Year: 2011

During inflammation, selectin-ligand interactions provide forces for circulating leukocytes to adhere to vascular surfaces, which stretch the interacting molecules, suggesting that mechanical properties may be pertinent to their biological function. From mechanical measurements with atomic force microscopy, we analyzed the molecular characteristics of selectins complexed with ligands and antibodies. Respective stiffness of L-, E-, and P-selectins (4.2, 1.4, and 0.85 piconewton/nm) correlated inversely with the number (2, 6, and 9) of consensus repeats in the selectin structures that acted as springs in series to dominate their compliance. After reconstitution into a lipid bilayer, purified membrane P-selectin remained a dimer, capable of forming dimeric bonds with P-selectin glycoprotein ligand (PSGL)-1, endoglycan-Ig, and a dimeric form of a glycosulfopeptide modeled after the N terminus of PSGL-1. By comparison, purified membrane L- and E-selectin formed only monomeric bonds under identical conditions. Ligands and antibodies were much less stretchable than selectins. The length of endoglycan-Ig was found to be 51 ± 12 nm. These results provide a comprehensive characterization of the molecular stiffness of selectins and illustrate how mechanical measurements can be utilized for molecular analysis, e.g. evaluating the multimericity of selectins and determining the molecular length of endoglycan. © 2011 by The American Society for Biochemistry and Molecular Biology, Inc. Source


Trademark
Aderans Research Institute Inc. | Date: 2009-01-20

Cells for medical or clinical use, namely, hair follicle cells to be used for hair transplants; Biological hair tissue intended for a subsequent hair transplant.


Trademark
Aderans Research Institute Inc. | Date: 2010-12-28

Cells for medical or clinical use, namely, hair follicle cells to be used for hair transplants; Biological hair tissue intended for a subsequent hair transplant.


Trademark
Aderans Research Institute Inc. | Date: 2013-09-17

Medical devices, namely, injection devices for the delivery of solutions, suspensions, fluids, and substances to a patient, for use in dermatologic procedures relating to hair growth or hair restoration.


Patent
Aderans Research Institute Inc. | Date: 2013-10-21

Compositions and methods for producing reconstituted human skin and/or hair follicles in situ are provided. The method for producing the skin is unique in that tissue culture expanded cells including multipotent cells such as neonatal cells as well as cultured epidermal and dermal cells are immediately placed on a substrate such as a membrane and then the membrane with adherent cells is placed on a skin wound. Examples demonstrate formation of hair follicles in situ.

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