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Patent
Achira Labs Pvt. Ltd | Date: 2010-09-03

In one aspect, the invention provides a method for making a hydrophilic-silk composition. The method includes providing at least one strand of silk fiber, treating the silk fiber with an alkaline solution to provide at least one strand of degummed silk fiber, and treating the degummed silk fiber with a treatment solution to provide a hydrophilic-silk drophilic-silk composition. The degummed silk fiber or the hydrophilic-silk composition is further immobilized with at least one reagent to make a silk-based diagnostic composition. The invention provides a silk-based diagnostic composition made by the method of the invention, and a diagnostic device that comprises the silk-based diagnostic composition. In another aspect, the invention provides a method of making a diagnostic device. The method includes providing at least one strand of a diagnostic-fiber composition, providing at least one strand of a hydrophobic-fiber composition, inter-weaving the at least one strand of the diagnostic-fiber composition and the at least one strand of the hydrophobic-fiber composition. In one embodiment, the diagnostic-fiber composition and the hydrophobic-fiber composition are both based on silk.


Modali A.,Achira Labs Pvt. Ltd. | Vanjari S.R.K.,Indian Institute of Technology Hyderabad | Dendukuri D.,Achira Labs Pvt. Ltd.
Electroanalysis | Year: 2016

In this paper, we demonstrate a woven electrochemical biosensor patch as a low cost, robust wearable sensor for non-invasive diagnostics. The critical requirement for wearable sensors is their robustness which necessitates the output signal to be independent of the deformation that the sensor system could potentially undergo. In this work, the performance of woven electrode patch post standard deformations such as bending and twisting were analysed using chronoamperometric detection of lactate, a sweat borne analyte, as a model system. In spite of rigorous deformation, the CV was well within 13 %, indicating the applicability of woven electrode patch as a low cost, robust, wearable, non-invasive electrochemical biosensor platform. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Source


Robinson A.M.,Saint Marys University, Halifax | Zhao L.,Saint Marys University, Halifax | Shah Alam M.Y.,Saint Marys University, Halifax | Bhandari P.,Achira Labs Pvt. Ltd. | And 4 more authors.
Analyst | Year: 2015

The demand for methods and technologies capable of rapid, inexpensive and continuous monitoring of health status or exposure to environmental pollutants persists. In this work, the development of novel surface-enhanced Raman spectroscopy (SERS) substrates from metal-coated silk fabric, known as zari, presents the potential for SERS substrates to be incorporated into clothing and other textiles for the routine monitoring of important analytes, such as disease biomarkers or environmental pollutants. Characterization of the zari fabric was completed using scanning electron microscopy, energy dispersive X-ray analysis and Raman spectroscopy. Silver nanoparticles (AgNPs) were prepared, characterized by transmission electron microscopy and UV-vis spectroscopy, and used to treat fabric samples by incubation, drop-coating and in situ synthesis. The quality of the treated fabric was evaluated by collecting the SERS signal of 4,4′-bipyridine on these substrates. When AgNPs were drop-coated on the fabric, sensitive and reproducible substrates were obtained. Adenine was selected as a second probe molecule, because it dominates the SERS signal of DNA, which is an important class of disease biomarker, particularly for pathogens such as Plasmodium spp. and Mycobacterium tuberculosis. Excellent signal enhancement could be achieved on these affordable substrates, suggesting that the developed fabric chips have the potential for expanding the use of SERS as a diagnostic and environmental monitoring tool for application in wearable sensor technologies. © The Royal Society of Chemistry 2015. Source


Bhandari P.,Achira Labs Pvt. Ltd. | Narahari T.,Achira Labs Pvt. Ltd. | Dendukuri D.,Achira Labs Pvt. Ltd.
Lab on a Chip - Miniaturisation for Chemistry and Biology | Year: 2011

Low cost and scalable manufacture of lab-on-chip devices for applications such as point-of-care testing is an urgent need. Weaving is presented as a unified, scalable and low-cost platform for the manufacture of fabric chips that can be used to perform such testing. Silk yarns with different properties are first selected, treated with the appropriate reagent solutions, dried and handloom-woven in one step into an integrated fabric chip. This platform has the unique advantage of scaling up production using existing and low cost physical infrastructure. We have demonstrated the ability to create pre-defined flow paths in fabric by using wetting and non-wetting silk yarns and a Jacquard attachment in the loom. Further, we show that yarn parameters such as the yarn twist frequency and weaving coverage area may be conveniently used to tune both the wicking rate and the absorptive capacity of the fabric. Yarns optimized for their final function were used to create an integrated fabric chip containing reagent-coated yarns. Strips of this fabric were then used to perform a proof-of-concept immunoassay with sample flow taking place by capillary action and detection being performed by a visual readout. © 2011 The Royal Society of Chemistry. Source


Choudhary T.,Achira Labs Pvt. Ltd. | Rajamanickam G.P.,Achira Labs Pvt. Ltd. | Dendukuri D.,Achira Labs Pvt. Ltd.
Lab on a Chip - Miniaturisation for Chemistry and Biology | Year: 2015

We present textile weaving as a new technique for the manufacture of miniature electrochemical sensors with significant advantages over current fabrication techniques. Biocompatible silk yarn is used as the material for fabrication instead of plastics and ceramics used in commercial sensors. Silk yarns are coated with conducting inks and reagents before being handloom-woven as electrodes into patches of fabric to create arrays of sensors, which are then laminated, cut and packaged into individual sensors. Unlike the conventionally used screen-printing, which results in wastage of reagents, yarn coating uses only as much reagent and ink as required. Hydrophilic and hydrophobic yarns are used for patterning so that sample flow is restricted to a small area of the sensor. This simple fluidic control is achieved with readily available materials. We have fabricated and validated individual sensors for glucose and hemoglobin and a multiplexed sensor, which can detect both analytes. Chronoamperometry and differential pulse voltammetry (DPV) were used to detect glucose and hemoglobin, respectively. Industrial quantities of these sensors can be fabricated at distributed locations in the developing world using existing skills and manufacturing facilities. We believe such sensors could find applications in the emerging area of wearable sensors for chemical testing. This journal is © The Royal Society of Chemistry 2015. Source

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