Groll J.,achen University
Methods in enzymology | Year: 2010
Poly(ethylene oxide) (PEO) is known as an excellent coating material to minimize nonspecific protein adsorption. For an examination of biomolecules attached to surfaces with sensitivities down to the single-molecule level, demands on the surface additionally comprise low-intrinsic fluorescence of the coating material and a possibility to immobilize biomolecules in their functional conformation. One strategy that combines the protein-resistant properties of PEO with chemical functionality is the use of star-shaped PEOs that allow for interpolymer cross-linking. Our system consists of six-arm PEO-based star polymers functionalized with reactive isocyanate groups at the ends of the polymer chain. The isocyante groups allow intermolecular cross-linking so that high grafting densities may be achieved, which render the surfaces extremely resistant to protein adsorption. Application by spin coating offers a simple procedure for the preparation of minimally interacting surfaces. The reactive end groups may be further biofunctionalized to recognize specific biomolecules such as streptavidin or His-tagged proteins in specific geometries or as single isolated molecules. These properties, together with the advantageous chemical properties of PEO, render the surfaces ideal for immobilizing proteins with detection limits down to the single molecule level. This chapter focuses on the preparation of substrates that are suitable for single-molecule experiments. Besides a detailed description of surface preparation, two examples for the single-molecule detection of immobilized proteins, nucleosomes and RNase H, are presented that demonstrate the advantages of the star-polymer derived coatings over linear-grafted PEO. Copyright 2010 Elsevier Inc. All rights reserved.
Gariboldi E.,Polytechnic of Milan |
Lemke J.N.,Polytechnic of Milan |
Ozhoga-Maslovskaja O.,achen University |
Timelli G.,University of Padua |
Bonollo F.,University of Padua
Metallurgia Italiana | Year: 2016
Hypo-or nearly eutectic Al-Si-Cu alloys are widely used as high pressure die casting alloys in foundry Due to high strength-to-weight ratio, they offer a wide range of applications. Some of the most commonly used Al-Si alloys are EN AC-46000, EN AC-46W0 and EN AC-47100 alloys. Their beneficial properties have been characterized in plenty previous works Less attention got their tensile behaviour at relatively high temperature, which is becoming of interest due to the possibility that the cast parts are applied at moderate temperatures and in the presence of external applied forces On the other hand the mechanical behaviour of these alloys at temperatures as high as those reached during possible homogenization or solution treatment processes could help modelling the thermomechanical behaviour of castings during heat treatments The research work focuses on the evolution of the tensile properties of diecast EN AC 46000-AlSi9Cu3(Fe), EN AC 46100-AISinCu2(Fe) and EN AC 47100-AlSi12Cu1(Fe)alloys Hot tensile testing with increasing temperature from room temperature up to 450°C were carried out on 3 mm thick specimen in view of proposing a temperature-dependent description of the main material properties.
Diessel D.,achen University |
Hirtz M.,achen University |
Otto N.,achen University |
Robens N.,achen University |
And 3 more authors.
Olhydraulik und Pneumatik | Year: 2013
A continuous improvement in the overall economic situation compared to previous years could also be felt in this year's leading trade fair MDA - Motion, Drive and Automation. In Hanover, numerous fluid power companies presented their latest innovations in hydraulics and pneumatics. The authors of the article draw a conclusion of personal highlights of the fair based on several selected examples.
PubMed | achen University
Type: Journal Article | Journal: Current pharmaceutical design | Year: 2013
Biosensors are used for a variety of applications in medicine and biology. A critical step during the development of such devices is the coordination of biological and technical requirements. The design of the device, as well as of the sample chamber and its functionalized surface is of great importance. Depending on the surface, the method of coupling of the desired receptor has to be adapted to guarantee functionality and biological activity during the measuring process. By using the SNAP-tag technology, a site-specific coupling of molecules with unaltered activity to a variety of O(6)-benzylguanine functionalized surfaces is possible, making it a versatile tool for the setup of biomedical devices.