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Sarkar D.,Acharya Bangalore B School ABBS | Laha S.,Acharya Bangalore B School ABBS
International Journal of Applied Environmental Sciences | Year: 2013

Antibiotics are one of the most important commercially exploited secondary metabolites roduced by the bacteria and employed in a wide range. Most of the antibiotic producers used today are the soil microbes. Fungal strains and streptomyces members are extensively used in industrial antibiotic production. Bacteria are easy to isolate, culture, main-tain and to improve their strains. Microbes are omni present and exist in a competitive environment. Bacillus species being the predominant soil bacteria because of their resistant endospore formation and production of vital antibiotics like bacitracin etc. are always found inhibiting the growth of the other organisms. In the present paper a soil bacterium with the antibiotic activity was screened and studied for morphological characters probably providing valuable information about the strain. The inhibitory activity of the organism was checked against some of the important opportunistic microbial flora and inoculated into an appropriate designed media depending on the bacterial requirements, and incubated for 48 hrs at 370C. The produced compound was extracted by solvent extraction and assayed for its activity. Enhancement in the anti-biotic production was studied under various parameters like temperature, pH, carbon source concentration, and sodium nitrate concentration, probably helping in the industrial production. The extracted substance was found effective against the gram positive endospore forming bacilli and gram positive cocci. Though a large list of antibiotics are known to be commercially available, the search for the most potential one is still on, and this work may provide some potential information on the antibiotic production and the control of microbial strains. © Research India Publications. Source


Sarkar D.,Acharya Bangalore B School ABBS | Laha S.,Acharya Bangalore B School ABBS
International Journal of Applied Environmental Sciences | Year: 2013

The phytohormone auxins play a central role in plant growth and development as a regulator of numerous biological processes, from cell division, elongation and differentiation to tropic responses, fruit development and senescence. Auxins are employed to induce rooting, callus formation, flowering, parthenocarpy and so on. They can also prevent abscission of leaves, flowers and fruits. The action and interaction of some growth regulators like auxins regulate most of the physiological activities and growth in plants. Naturally occurring substances with indole nucleus possessing growth-promoting activity are referred to as auxins, chemically it is Indole acetic acid.(IAA) Not only plants but also microorganisms can synthesize auxins and cytokinins. The ability to synthesize phytohormone is widely distributed among plantassociated bacteria. 80% of the bacteria isolated from plant rhizosphere produce IAA. Many bacteria are known to produce IAA. Hence the present paper discusses isolation of rhizobia from the selected leguminous plants and its IAA production ability under laboratory conditions. © Research India Publications. Source


Sarkar D.,Acharya Bangalore B School ABBS | Chaki S.,Acharya Bangalore B School ABBS
International Journal of Pharma and Bio Sciences | Year: 2013

Due to the development of increased resistance power of large number of fungal and bacterial pathogens to common antimicrobial agents, there is an urgent need to survey for new novel antimicrobial agents. In the present study the bacterial strain capable of producing antimicrobial agent was isolated from soil collected from the organic waste dump site. The isolated bacterial strain was further screened for their antifungal activity. The identified antifungal bacteria were Azomonas sp. DS. The metabolites of the isolated bacteria were screened for antifungal activity against ten different fungal species. Screening was done by using paper disc containing 20μl of culture supernatant placed on nutrient agar plate seeded with test organisms. The activity was determined by measuring the inhibition zone diameter (in cm) both for crude and dilution with 0.1 (M) phosphate buffer (pH-7.0) in the ratio of 1:1 (Crude extract:Buffer). The Azomonas sp. DS showed inhibition zone diameter of 6.3 cm against Beauveria sp. with the application of crude metabolite and dilution of crude metabolite with 0.1 (M) phosphate buffer (pH-7.0) in the ratio of 1:1 (Crude metabolite: Buffer) showed inhibition zone diameter of 4.0 cm. From this work it might be concluded that Azomonas sp. DS can be used for the production of novel antifungal agent. Further study will be needed for characterization of the metabolites. Source


Sarkar D.,Acharya Bangalore B School ABBS | Laha S.,Acharya Bangalore B School ABBS
International Journal of Pharma and Bio Sciences | Year: 2013

Lipase production in Aspergillus niger was tested using both submerged fermentation (SmF) and solid-state fermentation (SSF) on a mineral culture medium and wheat bran, respectively. Culture media was optimized in both SmF and SSF. We found 1.46 IU/mL was the optimum activity of lipase in case of submerged fermentation, when medium containing 2% glucose and 2% olive oil under the conditions of 1 vvm and 450 m-1. However, 9.14 IU/g of dry solid substrate equivalent to 4.8 IU/mL of lipase activity was reached using solid-state fermentation process with a medium containing 0.75 % of ammonium sulphate and 0.34 % of urea. The optimum pH and temperature for enzymatic activity were pH=6 and 40°C, respectively. When we used neutral and midly acid media, temperature ranges between 20 to 30°C for 24 hour, the enzyme showed 80% of its initial activity. Source


Sarkar D.,Acharya Bangalore B School ABBS | Laha S.,Acharya Bangalore B School ABBS | Chaki S.,Acharya Bangalore B School ABBS
International Journal of Pharma and Bio Sciences | Year: 2013

Research on Xylanase enzyme has markedly increased due to its potential applications in several industries include pulping and bleaching processes, where it is using cellulose free preparations, textile processes, the enzymatic saccharification of lignocellulosic materials and waste treatment. The present study was aimed at isolation and characterization of xylan degrading strain of Bacillus sp from soil for production of xylanase. Five isolates were obtained from soil samples of different areas in the Andhrahalli area of Bangalore, Karnataka and studied for detection of xylanase activity. One of the strains was identified as Bacillus sp and it was having close relation with Bacillus cereus. On the basis of the nucleotide sequence of the 16S rRNA gene, which produces xylanase extracellularly. In this work we had purified xylinase enzyme after treating ammonium sulphate and then that precipitation was used for purification. Purification was done by using DEAE-sepharadox and Hydroxyapatite column chromatography. The SDS-PAGE gave a single band at 32 kDa. For the purified enzyme, optimum temperature was found 30 C and pH was 6.0. The xylanase hydrolyzed oat spelt xylan, birch wood xylan and beech wood xylan efficiently but showed no activity towards cellulose, CM-cellulose. Thus it was a true and neutral xylanase. Reporting on isolation of xylanase from Bacillus sp is rare. Source

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