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News Article | May 24, 2017
Site: www.businesswire.com

SAN DIEGO--(BUSINESS WIRE)--Bacterial biofilms, surface adherent communities of bacteria that are encased in a secreted extracellular matrix of polymeric molecules, can cause diverse pathologies ranging from food poisoning and catheter infections to gum disease and the rejection of medical implants. Though the economic impact of biofilms is in the tens of billions of dollars per year, there is currently a paucity of means for preventing their formation or treating them once they are established. Compared to the free-floating “planktonic” lifestyle, within biofilms bacteria adopt differential gene expression profiles, have heightened resistance to antibiotics (up to 1,000-fold), and are less detectable/ accessible to the immune system. The current approach for treating biofilm infections is far from optimal: antibiotics are administered in a trial-and-error manner. To reduce the time required for eliminating a biofilm infection, lower the cost of treatment, and improve the patient’s experience, it would be helpful to empirically identify the most efficacious antibiotic prior to initiating treatment. Towards this end, Dr. Alex Mira and colleagues at the Centre for Advanced Research in Public Health (Valencia, Spain) recently announced an initiative to personalize the treatment of biofilm infections using a novel ex vivo antibiotic screening assay. In their protocol, bacteria from biofilm growing on a patient’s catheter, medical implant, infected gums, etc. are isolated and grown in specialized electronic microtiter plates (E-Plates®) in the presence or absence of diverse antibiotics. An xCELLigence instrument, which uses the principle of cellular impedance to track the real-time growth of both the bacteria and their extracellular matrix inside E-Plates, is then used to rank the efficacy of the different antibiotics tested. Using this approach, in just 8 hours a physician can identify the optimal therapeutic course of action on a patient by patient basis. The primary objective of this initiative’s ongoing first phase is to determine how accurate the ex vivo xCELLigence assay is for predicting antibiotic efficacy in patients. With the backing of Minister of Health Carmen Montón, the project has been fast-tracked and Mira’s team is expecting to enroll 100 patients over the first year. If the clinical findings reflect the preliminary data, this personalized approach will revolutionize how biofilm infections are treated in the near future. xCELLigence® Real Time Cell Analysis (RTCA) instruments utilize gold microelectrodes embedded in the bottom of microtiter wells to non-invasively monitor the status of adherent cells using the principle of cellular impedance. In short, cells act as insulators – impeding the flow of a miniscule electric current between electrodes. This impedance signal is measured automatically, at an interval defined by the user, and provides an extremely sensitive readout of cell number, cell size, cell-substrate attachment strength, and cell invasion/migration. xCELLigence® RTCA instruments are being used in both academia and industry for basic and applied applications ranging from bacterial biofilms and cancer immunotherapy to cardiotoxicity and drug discovery. To date xCELLigence® has been used in more than 1,250 publications, which can be viewed in a searchable library. Founded in 2002, ACEA Biosciences is a pioneer in the development and commercialization of high performance, cutting edge cell analysis platforms for life science research. ACEA’s xCELLigence® impedance-based, label-free, real-time cell analysis instruments and NovoCyte® flow cytometer are used in pre-clinical drug discovery and development, toxicology, safety pharmacology, and basic academic research. More than 2,300 instruments have been placed globally.


News Article | May 23, 2017
Site: www.prweb.com

Bacterial biofilms, surface adherent communities of bacteria that are encased in a secreted extracellular matrix of polymeric molecules, can cause diverse pathologies ranging from food poisoning and catheter infections to gum disease and the rejection of medical implants. Though the economic impact of biofilms is in the tens of billions of dollars per year, there is currently a paucity of means for preventing their formation or treating them once they are established. Compared to the free-floating “planktonic” lifestyle, within biofilms bacteria adopt differential gene expression profiles, have heightened resistance to antibiotics (up to 1,000-fold), and are less detectable/ accessible to the immune system. The current approach for treating biofilm infections is far from optimal: antibiotics are administered in a trial-and-error manner. To reduce the time required for eliminating a biofilm infection, lower the cost of treatment, and improve the patient’s experience, it would be helpful to empirically identify the most efficacious antibiotic prior to initiating treatment. Towards this end, Dr. Alex Mira and colleagues at the Centre for Advanced Research in Public Health (Valencia, Spain) recently announced an initiative to personalize the treatment of biofilm infections using a novel ex vivo antibiotic screening assay. In their protocol, bacteria from biofilm growing on a patient’s catheter, medical implant, infected gums, etc. are isolated and grown in specialized electronic microtiter plates (E-Plates®) in the presence or absence of diverse antibiotics. An xCELLigence instrument, which uses the principle of cellular impedance to track the real-time growth of both the bacteria and their extracellular matrix inside E-Plates, is then used to rank order the efficacy of the different antibiotics tested. Using this approach, in just 8 hours a physician can identify the optimal therapeutic course of action on a patient by patient basis. The primary objective of this initiative’s ongoing first phase is to determine how accurate the ex vivo xCELLigence assay is for predicting antibiotic efficacy in patients. With the backing of Minister of Health Carmen Montón, the project has been fast-tracked and Mira’s team is expecting to enroll 100 patients over the first year. If the clinical findings reflect the preliminary data, this personalized approach will revolutionize how biofilm infections are treated in the near future. About xCELLigence® RTCA xCELLigence® Real Time Cell Analysis (RTCA) instruments utilize gold microelectrodes embedded in the bottom of microtiter wells to non-invasively monitor the status of adherent cells using the principle of cellular impedance. In short, cells act as insulators – impeding the flow of a miniscule electric current between electrodes. This impedance signal is measured automatically, at an interval defined by the user, and provides an extremely sensitive readout of cell number, cell size, cell-substrate attachment strength, and cell invasion/migration. xCELLigence® RTCA instruments are being used in both academia and industry for basic and applied applications ranging from bacterial biofilms and cancer immunotherapy to cardiotoxicity and drug discovery. To date xCELLigence® has been used in more than 1,250 publications, which can be viewed in a searchable library. About ACEA Biosciences Founded in 2002, ACEA Biosciences is a pioneer in the development and commercialization of high performance, cutting edge cell analysis platforms for life science research. ACEA’s xCELLigence® impedance-based, label-free, real-time cell analysis instruments and NovoCyte® flow cytometer are used in pre-clinical drug discovery and development, toxicology, safety pharmacology, and basic academic research. More than 2,300 instruments have been placed globally. For more information, click here. For further information please contact:                                                                                     ACEA Biosciences, Inc.                                                                                                                 Dr. Jeff Xue                                                                                                                                     Phone: 1-858-724-0928, x3075                                                                                                 email: jxue(at)aceabio(dot)com


News Article | November 28, 2016
Site: www.prweb.com

ACEA Biosciences Inc. (ACEA), a privately owned biotechnology company focused on pioneering cell analysis instrumentation as well as the discovery and development of novel pharmaceuticals for the treatment of chronic diseases, today announced that Dr. Li Xu has joined the company as its Chief Medical Officer. Dr. Xu will lead ACEA’s clinical drug development team in both China and the USA. “We are very pleased to welcome Li during this exciting time for the company,” said Xiao Xu, M.D., President and Chief Executive Officer of ACEA. “As we continue the clinical development of our small molecules for treating cancer and autoimmune diseases, Li will contribute extensive strategic leadership, clinical development, and operational skill acquired within the realm of global clinical drug development. Her knowledge and experience in bringing innovative therapies from Phase I to regulatory approval will be a major asset as we continue advancing our EGFR TKI and BTK inhibitor programs towards commercialization." “I am delighted to join ACEA at this pivotal time point in the company's evolution as we seek for rapid and effective clinical development of ACEA’s innovative targeted therapies,” commented Dr. Xu. “I look forward to working closely with our scientific team and key opinion leaders to guide the advancement of these novel molecules – which may have the potential to address significant unmet medical needs and positively impact the lives of millions of patients with cancer and autoimmune disease." Dr. Li Xu has over 20 years of hands-on expertise leading global clinical development teams. Prior to joining ACEA, she was the Vice President and Head of the Oncology Business Unit at Jiangsu Hengrui Medicine Co. Ltd, in China. Prior to her role at Jiangsu Hengrui, Dr. Xu was the Vice President of the Oncology Business Unit and Head of Global Oncology Clinical Operations at Pfizer (2009-2013) focused on strategy, planning and execution; and the Director of Global Medical and Scientific Operations, Oncology Clinical Research Operations at Merck and Company (2006-2009). Dr. Li Xu has conducted/participated in multiple US, EU, and global phase I, II and III clinical trials, with 9 of these being US, EU, and global phase III trials. She has also provided strategic leadership to two very successful China FDA NDA registrations, including accelerated approval for Crizotinib (the first in class ALK inhibitor for ALK positive non-small cell lung cancer) during her time at Pfizer, and the approval for Apatinib (VEGF inhibitor for advanced GI cancers) during her time at Jiangsu Hengrui. About ACEA Biosciences ACEA Biosciences, Inc. (ACEA) is a privately owned biotechnology company focused on the development and commercialization of innovative cell analysis instrumentation for life sciences, and on the discovery and development of novel pharmaceutical products for the treatment of chronic diseases. ACEA’s instruments, xCELLigence® and NovoCyte™, are used in preclinical drug discovery and development, toxicology, safety pharmacology, disease studies, and basic academic research. More than 1,700 instruments have been placed globally, leading to >1,250 peer-reviewed publications from both academia and the pharmaceutical industry in diverse applications spanning everything from cancer immunotherapy and cardiotoxicity to chemotactic migration and GPCR inhibition. ACEA has leveraged its technology platform to develop a robust pipeline of clinical and preclinical small molecules for the treatment of cancer and autoimmune diseases, with two clinical stage programs running in China and the USA. ACEA Biosciences is headquartered in San Diego, California with world-class manufacturing operations in Hangzhou, China. For more information on ACEA’s ongoing clinical trials, click here.


News Article | February 28, 2017
Site: www.prweb.com

ACEA Biosciences, a pioneer in cell analysis instrumentation and the development of novel pharmaceuticals for the treatment of chronic diseases, has announced Dr. Roger (Feng) Luo as the new Vice President of Global Clinical Development. With more than 15 years of clinical development experience with a number of multinational drug companies, Dr. Luo will now team with Dr. Li Xu, Chief Medical Officer, and Dr. Xiao Xu, President, to continue advancing the company’s lead drug candidate, AC0010. The drug candidate is currently in clinical trials for the treatment of non-small cell lung cancer (NSCLC) in both China and the US. A pyrrolopyrimidine-based irreversible inhibitor of the EGFR tyrosine kinase, AC0010 provides NSCLC patients with a new treatment alternative should first generation EGFR tyrosine kinase inhibitors fail to produce a durable response. Thus far, AC0010 has demonstrated efficacy, tolerability, and safety in clinical trials in both China and the U.S. Global clinical trials are slated for late 2017. Before joining ACEA, Dr. Luo was Compound Development Team Lead, and Senior Leadership Member of Exploratory Medicine and Early Development at Johnson & Johnson (JNJ). Prior to JNJ, he was the Sr. Director of Early Clinical Development at Daiichi-Sankyo, and Head of the Tumor Pharmacology Group at Bristol-Myers Squibb. In total, Dr. Luo has participated in more than 20 oncology drug development trials around the globe, and has particular expertise in lung cancer therapeutics. “We are thrilled to have Roger join ACEA at this critical phase of the company’s history,” said Xiao Xu. “Roger’s experience and proficiency in drug development will be a major asset as we usher AC0010 through clinical trials.” For more information about ACEA Biosciences, click here. For further information please contact:                                                                                     ACEA Biosciences, Inc.                                                                                                                 Dr. Jeff Xue                                                                                                                                     Phone: +1 858 724 0928 x 3075                                                                                                 email: jxue(at)aceabio(dot)com


News Article | December 7, 2016
Site: www.prweb.com

ACEA Biosciences, Inc. presented today updated efficacy and safety data from its phase I/II dose escalation and expansion clinical trial for its lead drug candidate, AC0010, at the World Conference on Lung Cancer (WCLC) 2016 in Vienna, Austria. The purpose of the trial was to determine the safety, antitumor activity, and recommended phase II dosage of AC0010 in T790M‐postitive non-small cell lung cancer (NSCLC) patients after treatment with first generation EGFR tyrosine kinase inhibitors (TKIs) have failed to produce a durable response. AC0010 is an orally available, irreversible small molecule tyrosine kinase inhibitor in development for the treatment of patients with EGFRmut-positive NSCLC. It was designed specifically to inhibit epidermal growth factor receptor (EGFR) active mutations (L858R, Exon 19 del) and the T790M acquired resistance mutation. “Based on AC0010’s encouraging efficacy, tolerability, and distinct safety profile, it appears that this novel compound may provide an important treatment option for NSCLC patients who carry the EGFR T790M-positive resistance mutation,” said lead investigator of the trial Dr. Yi-Long Wu, MD, of the Guangdong Lung Cancer Institute, Guangdong General Hospital (GGH), and Guangdong Academy of Medical Sciences in Guangzhou, China. “Based on the good efficacy and tolerability, a 300 mg BID dose was selected as the RP2D.” “About 1.8 million new patients are diagnosed with lung cancer every year and, amongst these, 15-40% develop EGFR mutations. Considering these sobering numbers, we are very pleased with the preliminary efficacy and safety data for AC0010. The results presented today make us optimistic that further clinical trials of AC0010 as a treatment option for patients with advanced NSCLC is highly justified. We have initiated two registration trials in China, as well as a multicenter open-label phase I/II trial in the USA,” said Dr. Xiao Xu, President and CEO of ACEA Biosciences. “We will continue to work closely with patients, investigators, and regulatory agencies around the world to bring this important treatment option to NSCLC patients.” In the study presented at WCLC, oral AC0010 was administered on a 28‐day cycle with a starting dose of 50 mg twice per day (BID). In any given dose regimen, if 1 out of 3 patients demonstrated a partial response within the first cycle, and no dose-limiting toxicity was determined, up to 20 patients were subsequently enrolled. Plasma samples were collected to evaluate the pharmacokinetics of AC0010, and the presence of the T790M mutation in biopsy samples was evaluated by a central laboratory. The primary efficacy outcome measures for this analysis were objective response rate (ORR) and disease control rate (DCR), as assessed by the investigator according to RECIST v.1.1. Summary of Efficacy Data Using the international rules for Response Evaluation Criteria in Solid Tumors (RECIST) responses were observed at all dose levels except 50 mg BID. Amongst 158 patients evaluated in all regimens, the ORR (including unconfirmed responses) was 42%. In the dose cohorts between 200 mg BID and 300 mg BID (n=103 pts), the ORR and DCR were 50% and 90%, respectively. At 300 mg BID, which was selected as RP2D, a total of 48 patients were treated and the ORR and DCR were 52% and 94%, respectively. Pharmacokinetic analyses demonstrate rapid absorption with a Tmax of 2­4 hours and a median T1/2 of 7-8 hours. Summary of Safety Data As of 28 Oct 2016, 158 patients have been treated across 7 drug regimens (50, 100, 150, 200, 250, 300, and 350 mg BID). At the 28 Oct 2016 cutoff, 158 patients were evaluable. Maximum Tolerated Dose (MTD) has not been reached. The most common adverse events (AE) regardless of study drug relationship were diarrhea (43%), rash (28%), and ALT/AST elevation (44%/41%). Most AEs were grade 1 and 2. The most common Grade 3/4 drug related AEs were diarrhea (1%), rash (1%), and ALT/AST elevation (5%, 3%). All patients with grade 3/4 AEs recovered after either stopping the treatment or reducing the dose. As of the cutoff date, no cases of Grade 2/3 hyperglycemia were observed, and no patients had AEs leading to death. About AC0010 AC0010 is a pyrrolopyrimidine-based irreversible inhibitor of the EGFR tyrosine kinase which is in development for the treatment of NSCLC. Structurally distinct from previously reported pyrimidine-based irreversible EGFR inhibitors such as Osimertinib, AC0010 selectively inhibits EGFR active and T790M mutations up to a 298-fold more efficiently than wild-type EGFR. Multiple clinical trials currently underway in China and the USA are demonstrating the utility of AC0010 in this patient population. ACEA holds worldwide rights for AC0010. About ACEA Biosciences ACEA Biosciences, Inc. (ACEA) is a privately owned biotechnology company focused on the development and commercialization of innovative cell analysis instrumentation for life sciences, and on the discovery and development of novel pharmaceutical products for the treatment of chronic diseases. ACEA’s instruments, xCELLigence® and NovoCyte™, are used in preclinical drug discovery and development, toxicology, safety pharmacology, disease studies, and basic academic research. More than 1,700 instruments have been placed globally, leading to >1,250 peer-reviewed publications from both academia and the pharmaceutical industry in diverse applications spanning everything from cancer immunotherapy and cardiotoxicity to chemotactic migration and GPCR inhibition. ACEA has leveraged its technology platform to develop a robust pipeline of clinical and preclinical small molecules for the treatment of cancer and autoimmune diseases, with two clinical stage programs running in China and the USA. ACEA Biosciences is headquartered in San Diego, California with world-class manufacturing operations in Hangzhou, China. For more information on ACEA’s ongoing clinical trials please [click here.


News Article | December 1, 2016
Site: www.prweb.com

ACEA Biosciences, Inc. announced today that it will be presenting updated efficacy and safety data from its Phase I/II clinical trials for AC0010 at the World Conference on Lung Cancer 2016, taking place in Vienna, Austria December 3rd-8th. “We look forward to providing an update on the phase I/II clinical trials for AC0010 in patients with advanced non-small cell lung cancer harboring the EGFR T790M resistance mutation,” said Dr. Xiao Xu, President and CEO of ACEA. “These interim data demonstrate the promising clinical activity and tolerability profile of AC0010 in the treatment of NSCLC. The availability of alternative treatment options like AC0010 will provide patients and physicians with flexibility as they tailor treatment regimens based on efficacy and tolerability.” Presentation Detail Abstract 5117 - Phase I/II Study of AC0010, Mutant­Selective EGFR Inhibitor, in Non­Small Cell Lung Cancer (NSCLC) Patients with EGFR T790M Mutation. Presented by: Dr. Yi-Long Wu, MD, Guangdong Lung Cancer Institute, Guangdong General Hospital (GGH), and Guangdong Academy of Medical Sciences, Guangzhou/China. About AC0010 AC0010 is an orally available, irreversible small molecule tyrosine kinase inhibitor in development for the treatment of EGFR active and T790M mutations in NSCLC patients. In preclinical studies AC0010 has been found to selectively inhibit EGFR active (Exon 19 del, L858R) and T790M mutations up to 298-fold more efficiently than wild-type EGFR. Multiple clinical trials are currently underway in China and in the USA to demonstrate the clinical utility of AC0010 in NSCLC patients who have developed the EGFR 790M mutation. AC0010 is also being developed as a first-line therapy for treatment of the EGFR activating mutations Exon 19 del and L858R. ACEA holds worldwide rights for AC0010. About ACEA Biosciences ACEA Biosciences, Inc. (ACEA) is a privately owned biotechnology company focused on the development and commercialization of innovative cell analysis instrumentation for life sciences, and on the discovery and development of novel pharmaceutical products for the treatment of chronic diseases. ACEA’s instruments, xCELLigence® and NovoCyte®, are used in preclinical drug discovery and development, toxicology, safety pharmacology, disease studies, and basic academic research. More than 1,700 instruments have been placed globally, leading to >1,250 peer-reviewed publications from both academia and the pharmaceutical industry in diverse applications spanning everything from cancer immunotherapy and cardiotoxicity to chemotactic migration and GPCR inhibition. ACEA has leveraged its technology platform to develop a robust pipeline of clinical and preclinical small molecules for the treatment of cancer and autoimmune diseases, with two clinical stage programs running in China and the USA. ACEA Biosciences is headquartered in San Diego, California with world-class manufacturing operations in Hangzhou, China. For more information on ACEA’s ongoing clinical trials, click here.


News Article | November 1, 2016
Site: www.prweb.com

Book-ended by molecular analyses and live animal studies, cell-based assays are an indispensable tool for both basic and applied biological research. However, a long-standing challenge with most cell-based assays is that they only evaluate a single time point for just one molecular target. In contrast, the xCELLigence Real-Time Cell Analyzers produced by ACEA Biosciences enable cell health and behavior to be monitored continuously in a non-invasive manner. This technology maximizes the physiological relevance of data obtained in a wide variety of assays that include, but are not limited to, immune cell-mediated killing of cancer cells, cancer cell invasion/migration, predictive toxicology and cardiotoxicity, drug discovery, and immune cell activation. As part of its award announcement, CV Magazine interviewed Dr. Xiaobo Wang, ACEA’s Chief Technology Officer. Dr. Wang explained that from its inception a major goal of ACEA has been to “transform cell-based assays by improving the accuracy, reliability, and scope of cell measurements,” so that dynamic responses to small molecules, biomolecules, or other cells in culture can be evaluated. With nearly 1,800 xCELLigence instruments in use globally, leading to >1,250 publications in dozens of research fields, this technology has begun to represent the new standard for cell-based assays. For Dr. Wang’s complete interview on page 45 of October’s issue of CV Magazine, click here. About ACEA Biosciences Founded in 2002, ACEA Biosciences is a pioneer in the development and commercialization of high performance, cutting edge cell analysis platforms for life science research. ACEA’s xCELLigence® impedance-based, label-free, real-time cell analysis instruments and NovoCyte® flow cytometers are used in pre-clinical drug discovery and development, toxicology, safety pharmacology, and basic academic research. For more information, click here. For further information please contact:                                                                                     ACEA Biosciences, Inc.                                                                                                                 Dr. Jeff Xue                                                                                                                                     Phone: 1-858-724-0928, x3075                                                                                                 email: jxue(at)aceabio(dot)com


News Article | December 19, 2016
Site: www.prweb.com

The clinical classification of breast cancers is largely based upon the levels at which the patient’s cancerous cells express estrogen receptor and human epidermal growth factor receptor 2 (HER2), as determined by immunohistochemistry. Amplification of the HER2 gene and/or overexpression of the HER2 protein, which occurs in roughly 15-20% of breast cancers, is associated with more aggressive disease progression, metastasis, and a poorer prognosis. Therapeutics such as the monoclonal antibodies Trastuzumab and Pertuzumab, which bind HER2 and thereby prevent its dimerization and signaling activity, significantly improve clinical outcomes for HER2 overexpressing patients. Surprisingly, however, HER2 inhibitors have also been found to benefit some patients whose cancer cells do not overexpress HER2. It has been suggested that in these contexts it is aberrant HER2 activity, rather than increased HER2 expression, which accounts for the clinical benefit of HER2 inhibition. In order to test the above hypothesis, Lance Laing and colleagues at the University of Minnesota have evaluated the ability of Pertuzumab to inhibit HER2-mediated signaling in breast cancer cells that do not overexpress HER2. Patient-derived malignant and healthy cells were clonally expanded and HER2 expression levels were assessed using flow cytometry. To functionally characterize HER2-mediated signaling cells were exposed to HER2 ligand and their response recorded using one of ACEA Biosciences’ xCELLigence instruments – which monitor cell number, size, and cell-substrate attachment quality in real-time using the principle of cellular impedance. HER2 stimulation by the ligand was found to rapidly increase the impedance signal (within minutes). Importantly, some primary breast cancer cells that do not overexpress HER2 still displayed a functional response to ligand stimulation. This lends support to the hypothesis that aberrant HER2 signaling in the absence of overexpression may leave some breast cancer cells still susceptible to HER2-targeted therapies. Consistent with this, the HER2 response detectable by xCELLigence could be suppressed in a dose-dependent manner by Pertuzumab. Their complete findings, published in the recent issue of the journal Oncotarget, can be viewed here. The data from this study suggest that cell-based functional assays, as opposed to mere biomarker evaluation, may provide a more accurate means of determining the optimal therapeutic strategy for each patient. Learn more about xCELLigence RTCA and how it is being used for cancer immunotherapy research. About xCELLigence® ACEA’s xCELLigence® Real Time Cell Analysis (RTCA) instruments utilize gold microelectrodes embedded in the bottom of microtiter wells to non-invasively monitor the status of adherent cells using the principle of cellular impedance. In short, cells act as insulators – impeding the flow of an alternating microampere electric current between electrodes. This impedance signal is measured automatically, at an interval defined by the user (e.g. every 10 seconds, once per hour, etc.), and provides an extremely sensitive readout of cell number, cell size/shape, and cell-substrate attachment strength. About ACEA Biosciences Founded in 2002, ACEA Biosciences is a pioneer in the development and commercialization of high performance, cutting edge cell analysis platforms for life science research. ACEA’s xCELLigence® impedance-based, label-free, real-time cell analysis instruments and NovoCyte® flow cytometer are used in pre-clinical drug discovery and development, toxicology, safety pharmacology, and basic academic research. More than 2,000 instruments have been placed globally, leading to >1,200 peer reviewed publications. For further information please contact:                                                                                     ACEA Biosciences, Inc.                                                                                                                 Dr. Jeff Xue                                                                                                                                     Phone: +1 858 724 0928 x 3075                                                                                                 email: jxue(at)aceabio(dot)com


News Article | November 11, 2016
Site: www.prweb.com

In addition to living in a free-floating “planktonic” state within aqueous environments, bacteria can also colonize solid surfaces present at liquid-solid and air-solid interfaces. Within these microenvironments the bacteria “speak” to each other via chemical messengers to coordinate gene expression profiles in ways that promote survival of the colony. A common feature of these bacterial communities is the secretion of polymeric substances (polysaccharides, nucleic acids, proteins, etc.) which serve to encapsulate the cells and protect them from the environment. These “biofilms” enhance antibiotic resistance as much as 10,000-fold and play critical roles in human health and disease, including: dental plaques and cavities, infections, rejection of artificial implants, and food poisoning. Though developing drugs to treat biofilms – or prevent their formation in the first place – is of critical importance, existing assays for assessing biofilm formation and growth are time consuming and low throughput. Microtiter plate endpoint assays wherein adherent bacteria are stained with crystal violet are currently the most common type of assay used. Last month Patricia Ruas-Madiedo and colleagues demonstrated in the journal PLOS ONE use of the xCELLigence Real-Time Cell Analysis (RTCA) technology for monitoring the biofilms produced by clinically relevant bacterial species including Staphylococcus epidermidis and Streptococcus mutans. Biofilm-producing strains could readily be differentiated from non-producing strains using this technique. Importantly, the authors showed that treating bacteria either before or after biofilm production with cell wall-degrading endolysin enzymes or with bacteriophage result in a decreased xCELLigence signal that correlates with mitigation of the bacteria. These proof of concept studies represent a major step forward as they demonstrate the ability to quantitatively track in a continuous manner the state of biofilms without having to use labels or labor intensive protocols. Adoption of this approach has the potential to dramatically accelerate screening programs focused on discovery/development of drugs for preventing biofilm formation or destroying it once it has been established. Learn more about xCELLigence RTCA, and how it is being used for diverse applications. About xCELLigence® ACEA’s xCELLigence® Real Time Cell Analysis (RTCA) instruments utilize gold microelectrodes embedded in the bottom of microtiter wells to non-invasively monitor the status of adherent cells using the principle of cellular impedance. In short, cells act as insulators – impeding the flow of an alternating microampere electric current between electrodes. This impedance signal is measured automatically, at an interval defined by the user (e.g. every 10 seconds, once per hour, etc.), and provides an extremely sensitive readout of cell number, cell size/shape, and cell-substrate attachment strength. About ACEA Biosciences Founded in 2002, ACEA Biosciences is a pioneer in the development and commercialization of high performance, cutting edge cell analysis platforms for life science research. ACEA’s xCELLigence® impedance-based, label-free, real-time cell analysis instruments and NovoCyte® flow cytometers are used in pre-clinical drug discovery and development, toxicology, safety pharmacology, and basic academic research. For more information, click here. For further information please contact:                                                                                     ACEA Biosciences, Inc.                                                                                                                 Dr. Jeff Xue                                                                                                                                     Phone: +1 858 724 0928 x 3075                                                                                                 email: jxue(at)aceabio(dot)com


Hu N.,Zhejiang University | Wu C.,Zhejiang University | Ha D.,Zhejiang University | Wang T.,ACEA Biosciences, Inc. | And 2 more authors.
Biosensors and Bioelectronics | Year: 2013

This study presents a novel microphysiometer for studying the mechanism of cellular metabolism and drug effect. Based on the photocurrent amplification of light-addressable potentiometric sensor (LAPS), the constant voltage detection mode was introduced to enhance the detection sensitivity to replace the conventional constant current mode with the slow feedback rate. The photocurrent amplification of LAPS was improved by developing the sensor structure and fabrication processes. The sensor unit with microfluidic system was designed to detect the concentration change of cellular acidic metabolites in the extracellular microenvironment rapidly. Characteristic test experiments and cellular metabolism experiments were carried out to determine the performance of microphysiometer. The result showed that sensitivity of microphysiometer is significantly enhanced to sense the fluctuation of cellular metabolism rapidly and sensitively in real-time detection of living cells under physiological condition. With these improvements, the novel microphysiometer holds promise as a utility platform for studying cellular metabolism and evaluating drug effect. © 2012 Elsevier B.V.

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