Wei F.,Academy of Shanxi Medical Science |
Ma L.-M.,Academy of Shanxi Medical Science |
Gong X.-D.,Academy of Shanxi Medical Science |
Ren L.-S.,Academy of Shanxi Medical Science |
And 3 more authors.
Journal of Leukemia and Lymphoma | Year: 2013
Objective To establish a mouse model of acute graft-versus-host disease (aGVHD) after allogeneic bone marrow transplantation, and using exogenous interleukin-7 receptor alpha (IL-7Rα) intervene mice aGVHD and analyse its possible mechanism. Methods The BALB/C (H-2d) female mice as recipients were grouped by rat: the irradiation group (group A), irradiation transplantation group (group B) and IL-7Ra in the intervention group (group C), each 10. ALL mice were accepted 9 Gy 60Co total body irradiation. 1×1O7 bone marrow cells and 2×107 spleen cells of donor C57BL/6 (H-2b) via the tail vein were infused to recipient mice. The signs of the recipient mice,hematopoietic functional recovery and survival time of change, and pathology, chimerism and cytokine levels in check were observed. Results Mice in A group after irradiation were gradually death, in group B and group C mice after transplantation had typical aGVHD symptoms, but lighter signs and a longer survival time of Group C than in group B. WBC count in Group C was +14 d (4.53 ± 0.21) x109/L, +21 d (3.63±0.06) x109/L, +28 d (4.31±0.04) x109/L, was hematopoietic recovery compared with Group B [+14 d (1.81±0.05) x109/L, +21 d (1.32±0.04) x109/L, +28 d (1.76±0.04) x109/L], the difference was statistically significant (t = 0.237, 0.108, 0.359, P < 0.05). The pathological results of liver, spleen, skin histopathology in group C were better than group B. Chimera implants, plasma IL-7 levels after transplant +7 d, concentration was significantly increased. IL-7 concentration in group C was +14 d (194.32±1.02) pg/ml, +21 d (131.63±1.54) pg/ml and in group B was +14 d (330.24±8.08) pg/ml, +21 d (184.09±2.05) pg/ml, the difference was statistically significant (t = 1.590, 1.285, P <0.05). Conclusion The stable aGVHD mouse model was established. In aGVHD early, plasma IL-7 levels were significantly increased. Exogenous IL-7Rα can reduce the plasma IL-7 levels, thereby reducing the incidence of aGVHD after allogeneic bone marrow transplantation.