AcSIR Academy of Scientific & Innovative Research

Delhi, India

AcSIR Academy of Scientific & Innovative Research

Delhi, India
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Palaniswamy S.K.,AcSIR Academy of Scientific & Innovative Research | Palaniswamy S.K.,CSIR - Central Electrochemical Research Institute | Govindaswamy V.,AcSIR Academy of Scientific & Innovative Research | Govindaswamy V.,CSIR - Central Electrochemical Research Institute
Enzyme and Microbial Technology | Year: 2017

We report here, for the first time, a zymogram technique designed for rapid screening of feruloyl esterase using ethyl ferulate as enzyme substrate and casein precipitation as enzyme activity indicator. © 2017 Elsevier Inc.


Banerji B.,CSIR - Central Electrochemical Research Institute | Banerji B.,AcSIR Academy of Scientific & Innovative Research | Killi S.K.,CSIR - Central Electrochemical Research Institute | Katarkar A.,Indian Institute of Science | And 4 more authors.
Bioorganic and Medicinal Chemistry | Year: 2017

Neo-tanshinlactone (NTL) a natural product is known for its specificity and selectivity towards the breast cancer cells. By NTL D-ring modification approach, 13 new analogues were synthesized (1A–1M). Among them 1J showed the best anticancer activity in MCF-7 (ER+, PR+/−, HER2−), SKBR3 (ER−, PR−, HER2+) and MDA-MB-231 (ER−, PR−, HER2−) cells lines with IC50value 11.98 nM, 23.71 nM, and 62.91 nM respectively. 1J showed minor grove binding interaction with DNA at AT-rich region and induced DNA double strand breaks (DDSBs). This had triggered several key molecular events involving, activation of ATM, Chk2 and p53, reduction in mitochondrial potential (Δψm) leading to caspase-3 and PARP cleavage mediated apoptosis. These results along with other biochemical studies strongly suggest that novel NTL analogue 1J caused DNA cleavage mediated apoptosis in the breast cancer cells and this may serve as potential lead for future breast cancer treatment. © 2016

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