Samantara A.K.,CSIR - Institute of Minerals And Materials Technology |
Samantara A.K.,Academy of Scientific and Innovative ResearchNew Delhi |
Chandra Sahu S.,CSIR - Institute of Minerals And Materials Technology |
Chandra Sahu S.,Academy of Scientific and Innovative ResearchNew Delhi |
And 3 more authors.
Journal of Materials Chemistry A | Year: 2015
Here, a hybrid material composed of sandwiched reduced graphene oxide (rGO) and N,S co-doped carbon quantum dots (N,S-CQDs) was prepared following a facile synthetic route. This metal-free composite has demonstrated their dual performance as an electrode material for supercapacitors and fuel cell catalysts. It shows robust cyclic stability with high energy and power density without any binders. The enhanced activity of the composite can be ascribed to the CQDs present within the interlayer of the rGO which enhance the accessibility of the charged ions and increase the capacitance of the composite. In addition, the electrocatalytic activity of the composite has been assessed as a metal-free cathode catalyst towards the oxygen reduction reaction (ORR) for fuel cell applications. It shows better performance in terms of high reduction potential and high reduction current as compared to rGO. This can be ascribed to the significant contribution of electron rich heteroatom doped CQDs as well as the synergistic effect of both the CQDs and rGO for the electrocatalytic reduction of oxygen. The interesting dual performance of heteroatom doped CQDs and graphene hybrid composites hold potential for the development of energy storage and conversion devices. This journal is © The Royal Society of Chemistry.
Bhosale M.E.,CSIR - National Chemical Laboratory |
Bhosale M.E.,Academy of Scientific and Innovative ResearchNew Delhi |
Illathvalappil R.,CSIR - National Chemical Laboratory |
Illathvalappil R.,Academy of Scientific and Innovative ResearchNew Delhi |
And 4 more authors.
Chemical Communications | Year: 2015
Conjugated porous polymers were used as precursors to prepare nitrogen and sulphur doped carbon atoms, which were then used for oxygen reduction and energy storage. © The Royal Society of Chemistry 2016.
Padhi B.,Indian Institute of Chemical Technology |
Padhi B.,Academy of Scientific and Innovative ResearchNew Delhi |
Reddy D.S.,Indian Institute of Chemical Technology |
Mohapatra D.K.,Indian Institute of Chemical Technology |
Mohapatra D.K.,Academy of Scientific and Innovative ResearchNew Delhi
RSC Advances | Year: 2015
An efficient gold(iii)-catalyzed general method for the diastereoselective allylation of six-membered cyclic hemiacetals at room temperature is developed. The present protocol is operationally simple with good functional group tolerance, thus providing easy access to various multifunctionalized 2,6-trans-disubstituted tetrahydropyrans. Since an allyl moiety is readily introduced, the developed strategy is highly versatile and has great potential for further functional group manipulation. Furthermore, a diastereoselective synthesis of the C1-C13 fragment of bistramide A and B has been described highlighting this methodology. © The Royal Society of Chemistry.
Bajpai V.,CSIR - Central Electrochemical Research Institute |
Bajpai V.,Academy of Scientific and Innovative ResearchNew Delhi |
Singh A.,CSIR - Central Electrochemical Research Institute |
Singh A.,Academy of Scientific and Innovative ResearchNew Delhi |
And 4 more authors.
Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment | Year: 2015
Adulteration or substitution of commercial Berberis aristata and its herbal products with inferior-quality substituents is very common. Metabolic profiling of B. aristata, along with its common adulterants/contaminants/substituents such as B. asiatica, Mahonia borealis and Coscinium fenestratum, was rapidly carried out using direct analysis in real-time mass spectrometry (DART MS) to generate the chemical fingerprints for the differentiation of these species. Phytochemical analysis showed the presence of mainly alkaloids. The identified alkaloids were berberrubine, berberine, jatrorrhizine, ketoberberine, palmatine, dihydropalmatine or 7,8-dihydro-8-hydroxyberberine, berbamine and pakistanamine. Berberine, which was mainly reported from the root and stem bark of B. aristata, was also identified in the leaf along with chlorogenic acid. The DART MS data have been subjected to principal component analysis (PCA). The resulting score plots showed clustering and clear differentiation of the species and plant parts. It is thus apparent that the technique of DART MS followed by PCA is a quick and reliable method for the direct profiling of B. aristata and its adulterant plants and plant parts. The study reports the rapid analytical method to identify the possibility of illegal adulteration/contamination/substitution in potential plant materials and herbal extracts. © 2015, © 2015 Central Drug Research Institute, India. Published by Taylor & Francis.
Sharma A.,CSIR - Central Electrochemical Research Institute |
Umar S.,CSIR - Central Electrochemical Research Institute |
Kar P.,CSIR - Central Electrochemical Research Institute |
Singh K.,CSIR - Central Electrochemical Research Institute |
And 3 more authors.
Analyst | Year: 2016
We discovered a new class of nontoxic, highly fluorogenic and biocompatible probe AFN for selective staining of intracellular Lipid Droplets (LDs) in both fixed and live human cervical cancer cells (HeLa) and 3T3-L1 pre-adipocytes without any background artifacts. The salient features of the probe are its visible excitation maximum, aqueous compatibility, selectivity and remarkable stability (for more than a week) in live cells, even better than commercially available Nile red. © The Royal Society of Chemistry.
Singh P.,Academy of Scientific and Innovative ResearchNew Delhi |
Singh P.,Indian Institute of Integrative Medicine |
Dar M.S.,Academy of Scientific and Innovative ResearchNew Delhi |
Dar M.S.,Indian Institute of Integrative Medicine |
And 10 more authors.
Journal of Cellular Biochemistry | Year: 2016
Cancer therapeutics is a hot subject and PI3K class 1A isoforms (p110α and p110β) are pursued as major targets. Genetic analysis, biochemical approaches, and structural studies have demonstrated crucial roles for these isoforms in several physiological processes. p110α is critical for insulin signaling, whereas p110β is essential for the growth and differs from p110α in many ways. Here, we have generated GFP-fusion clones of wildtype and mutant version of p110α and p110β and expressed them in HEK293 and cancer cells to examine their subcellular localization and their impact on downstream signaling. In HEK293 cells, p110β GFP-fusion protein is translocated into the nucleus, whereas p110α-GFP stays exclusively in the cytoplasm. This study demonstrates that p110α and p110β oncogenecity, kinase activity, and interaction with p85 regulatory subunit does not have any impact on their subcellular localization. PI3K pathway specific inhibitor, LY294002, abrogated PI3K signaling by reducing pAkt levels, however, the subcellular localization of p110α and p110β remained unchanged. Furthermore, we analyzed the expression of recombinant p110α and p110β in a panel of human cancer cells and observed remarkable differences in their expression levels. The differential expression of recombinant p110α and p110β was observed to be mainly regulated by the endogenous levels of pAkt. Unlike in HEK293, p110α showed nuclear localization in cancer cells in a similar fashion to p110β. Moreover, we observed the PI3K signaling activities in low pAkt expressing cells are mediated by PDK1 and S6K proteins. Finally, p110α and p110β were seen to play an essential role in promoting the cell cycle progression in MCF-7 and HCT-116 cells. J. Cell. Biochem. 117: 2864–2874, 2016. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.
Taneja I.,Academy of Scientific and Innovative ResearchNew Delhi |
Taneja I.,CSIR - Central Electrochemical Research Institute |
Raju K.S.R.,Academy of Scientific and Innovative ResearchNew Delhi |
Raju K.S.R.,CSIR - Central Electrochemical Research Institute |
And 9 more authors.
RSC Advances | Year: 2015
Alda-1 is an activator of the enzyme ALDH2. It has been suggested as a novel therapeutic for cardiovascular implications such as myocardial infarction, coronary bypass surgery, heart transplantation, peripheral artery disease, ischemia reperfusion injury, angina and alcoholic cardiomyopathy. Despite its widespread experimental use, no reports are available on its pharmacokinetics or bioanalytical quantification. In the present study, a simple, precise and reliable LC-ESI-MS/MS method has been developed and validated for the first time for quantification of alda-1 in plasma. Alda-1 was analyzed on a C18 column using methanol and 0.1% formic acid (60 : 40, v/v) as the mobile phase at a flow rate of 0.7 mL min-1. The method was found to be linear within the concentration range of 1-500 ng mL-1. The intra- and inter-day precision and accuracy were within acceptable limits. For the first time, the preclinical oral and intravenous pharmacokinetics of alda-1 were conducted. Alda-1 was found to be a rapidly absorbed, high clearance and poorly bioavailable compound in rats. Its plasma protein binding was found to be 82-86%. In view of the new regulatory guidelines, incurred sample reanalysis was also performed and all the samples were found within 15% of the mean value. From the in vitro microsomal incubation studies, it was found to be a high extraction compound. The data presented here provide important information to support the in vivo efficacy of alda-1 and would be helpful in its further development as a therapeutic agent and synthesis of its analogs with better systemic exposure and disposition properties. This journal is © The Royal Society of Chemistry.
Mandal S.,Indian Central Mechanical Engineering Research Institute |
Mandal S.,Academy of Scientific and Innovative ResearchNew Delhi |
Pal A.,Indian Central Mechanical Engineering Research Institute |
Arun R.K.,Indian Central Mechanical Engineering Research Institute |
And 2 more authors.
Journal of Electroanalytical Chemistry | Year: 2015
Abstract We report a new method for the fabrication of flexible supercapacitor electrodes wherein mechanically exfoliated graphite on paper containing gold nanoparticles is used as supercapacitor electrodes. It has been found that the supercapacitor electrode made of paper containing gold nanoparticle with graphite exfoliation yields a capacitance, which is about 4.3 times higher than that of the electrodes without gold nanoparticles. © 2015 Elsevier B.V.
Verma S.,CSIR - Central Electrochemical Research Institute |
Arha D.,CSIR - Central Electrochemical Research Institute |
Arha D.,Academy of Scientific and Innovative ResearchNew Delhi |
Tamrakar A.K.,CSIR - Central Electrochemical Research Institute |
And 3 more authors.
Current Topics in Medicinal Chemistry | Year: 2015
Bioactivity guided separation of combined n-hexane and chloroform extracts of Oplismenus burmannii resulted in the isolation and characterization of five new glycoglycerolipids, (2S)-1,2,6’-tri-O-hexadecanoyl-3-O-β-D-galactopyranosyl glycerol (1a), (2S)-1,2,6’-tri-O-[(9Z,12Z)-octadeca-9,12-dienoyl]-3-O-β-D-galactopyranosyl glycerol (1b), (2S)-1,6’-di-O-[(9Z,12Z)-octadeca-9,12-dienoyl]-3-O-β-D-galactopyranosyl glycerol (2b), (2S)-1,6’-di-O-[(9Z,12Z,15Z)-octadeca-9,12,15-trienoyl]-3-O-β-D-galactopyranosyl glycerol (2c), and (2S)-1,2-di-O-[(9Z,12Z)-octadeca-9,12-dienoyl]-3-O-(6-sulpho-α-D)-quinovopyranosyl glycerol (3b) along with five known glycoglycerolipids (1c, 2a, 3a, 3c and 4), a cerebroside (5), three monoacylglycerols (6a-c) and α-linoleic acid (7). The isolated compounds, 1-5 were in-vitro tested for their antihyperglycemic potential in terms of increase in 2-deoxyglucose uptake in L6-GLUT4myc myotube cells. The results showed that compounds, 1-5 were showing 1.52 (P<0.05), 1.50 (P<0.05), 1.28, 1.49 (P<0.05) and 1.50 (P<0.05) fold increase in the glucose uptake at concentration of 10 μg/mL and 1.71 (P<0.001), 1.74 (P<0.001), 1.50 (P<0.05), 1.76 (P<0.001) and 1.74 (P<0.001) fold increase in the glucose uptake at concentration of 25 μg/mL respectively. However, standard drug Rosiglitazone increases the glucose uptake by 1.59 fold at the concentration of 10μM. Further work on optimization of the anti-diabetic lead is under progress. © 2015 Bentham Science Publishers.
PubMed | Academy of Scientific and Innovative ResearchNew Delhi
Type: | Journal: Frontiers in plant science | Year: 2017
Universal stress proteins (USPs) are known to be expressed in response to various abiotic stresses in a wide variety of organisms, such as bacteria, archaebacteria, protists, algae, fungi, plants, and animals. However, in plants, biological function of most of the USPs still remains obscure. In the present study,