Tarakanova Y.N.,Academy of science of Bashkortostan |
Dmitriev D.A.,Academy of science of Bashkortostan |
Massino Y.S.,Academy of science of Bashkortostan |
Smirnova M.B.,Russian Academy of Sciences |
And 6 more authors.
Applied Biochemistry and Microbiology | Year: 2012
The dependence of the antigen-binding activity of immobilized antibodies on pH of a saturating buffer has been investigated. We analyzed 28 monoclonal antibodies (MCAs) produced by various hybridomas to three virus antigens, i.e., the nuclear p23 protein of hepatitis C virus (C core protein p23), p24 protein of HIV 1, and the surface antigen of hepatitis B virus (HBsAg). Antibodies were adsorbed on the surfaces of immune plates in acidic (pH 2.8), neutral (pH 7.5), and alkaline (pH 9.5) buffers. The binding of labeled antigens, i. e., biotinylated or conjugated with horseradish peroxidase, with immobilized antigens was tested. It was shown that 10 out of 28 analyzed MCAs (36%) considerably better preserved their antigen-binding activity if their passive adsorption was carried out on the surface of polystyrene plates in an acidic buffer (pH 2.8). This approach allowed constructing a highly sensitive sandwich method for HBsAg assay with a minimal reliably determined antigen concentration of 0.013-0.017 ng/ml. The described approach may be recommended for the optimization of sandwich methods and solid-phase competitive methods. © 2012 Pleiades Publishing, Ltd.
Migranov N.,Bashkir State University |
Kudreyko A.,Ufa State Petroleum Technological University |
Kondratyev D.,Academy of science of Bashkortostan
Physics Research International | Year: 2014
We propose a new theoretical approach for description of the equilibrium and bistable states of ferroelectric liquid crystal. A sample of ferroelectric liquid crystal is considered in the "bookshelf " geometry under the influence of electric field. Continuumand perturbation theories are used in order to establish the relation between the scale coefficients in the expansion for the azimuthal angle. The equilibrium states of ferroelectric SmC. are determined in the framework of the catastrophe theory. Copyright © 2014 Nail Migranov et al.
Baktybaeva Z.B.,Academy of science of Bashkortostan |
Yamalov S.M.,Bashkir State University |
Suyundukov Y.T.,Academy of science of Bashkortostan
Russian Journal of Ecology | Year: 2011
Geobotanical analysis of aquatic and wetland plant communities of the Tanalyk River has been performed in the zone of impact from the abandoned Kul-Yurt-Tau pyrite mine. The results show that pollution of the river with highly mineralized runoff leads to a decrease in the β-diversity and synthetic characteristics of plant communities (α-diversity, total coverage, and herb layer height) and in the aboveground phytomass of dominant species. © 2011 Pleiades Publishing, Ltd.
Filippov A.I.,Academy of science of Bashkortostan |
Akhmetova O.V.,Ufa State Aviation Technical University |
Gubaidullin M.R.,Academy of science of Bashkortostan
Journal of Engineering Physics and Thermophysics | Year: 2015
Simple analytical formulas for definition of the pressure field, formed in the process of continuous withdrawal of a fluid from a nonuniform anisotropic stratum, in the zero and first approximations on the assumption that the fluid flow in the stratum is one-dimensional and linear were derived on the basis of the modified asymptotic method exact on average. The results of calculations of the pressure fields in such stratums by the formulas obtained are presented. ©2015 Springer Science+Business Media New York.