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Kong D.,Jiangnan University | Liu L.,Jiangnan University | Song S.,Jiangnan University | Suryoprabowo S.,Jiangnan University | And 4 more authors.
Nanoscale | Year: 2016

A semi-quantitative and quantitative multi-immunochromatographic (ICA) strip detection assay was developed for the simultaneous detection of twenty types of mycotoxins from five classes, including zearalenones (ZEAs), deoxynivalenols (DONs), T-2 toxins (T-2s), aflatoxins (AFs), and fumonisins (FBs), in cereal food samples. Sensitive and specific monoclonal antibodies were selected for this assay. The semi-quantitative results were obtained within 20 min by the naked eye, with visual limits of detection for ZEAs, DONs, T-2s, AFs and FBs of 0.1-0.5, 2.5-250, 0.5-1, 0.25-1 and 2.5-10 μg kg-1, and cut-off values of 0.25-1, 5-500, 1-10, 0.5-2.5 and 5-25 μg kg-1, respectively. The quantitative results were obtained using a hand-held strip scan reader, with the calculated limits of detection for ZEAs, DONs, T-2s, AFs and FBs of 0.04-0.17, 0.06-49, 0.15-0.22, 0.056-0.49 and 0.53-1.05 μg kg-1, respectively. The analytical results of spiked samples were in accordance with the accurate content in the simultaneous detection analysis. This newly developed ICA strip assay is suitable for the on-site detection and rapid initial screening of mycotoxins in cereal samples, facilitating both semi-quantitative and quantitative determination. © The Royal Society of Chemistry 2016. Source

Wang W.,Jiangnan University | Wang W.,Academy of Science and Technology of State Administration of Grain | Liu L.,Jiangnan University | Xu L.,Jiangnan University | And 3 more authors.
ACS Applied Materials and Interfaces | Year: 2016

A sensitive surface-enhanced Raman scattering (SERS) immunosensor based on the Au nanoparticle (Au NP) shell structure was developed to detect staphylococcal enterotoxin B (SEB) on a microplate. Au NPs modified with 4-nitrothiophenol (4-NTP) and coated with Ag shell of controlled thickness at 6.6 nm exhibited excellent SERS intensity and were used as signal reporters in the detection of SEB. The engaged 4-NTP allowed the significant electromagnetic enhancement between Au NPs and the Ag shell and prevented the dissociation of the Raman reporter. More importantly, 4-NTP-differentiated SERS signals between the sample and microplate. The SERS-based immunosensor had a limit of detection of 1.3 pg/mL SEB. Analysis of SEB-spiked milk samples revealed that the developed method had high accuracy. Therefore, the SERS-encoded Au@Ag core-shell structure-based immunosensor is promising for the detection of biotoxins, pathogens, and environmental pollutants. © 2016 American Chemical Society. Source

Tang L.,Jiangnan University | Li S.,Jiangnan University | Han F.,Academy of Science and Technology of State Administration of Grain | Liu L.,Jiangnan University | And 6 more authors.
Biosensors and Bioelectronics | Year: 2015

Dopamine (DA) is a neurotransmitter which plays a key role in the life science. Self-assembled Au@Ag nanorod dimers based on aptamers were developed for ultrasensitive dopamine detection. The electronic field was significantly enhanced by the addition of silver shell coating on the surface of Au NR dimer. The results displayed that Au@Ag NR dimers were ideal building blocks for constructing the SERS substrates with prominent Raman enhancement effects. It was found that with using this Surface-enhanced Raman scattering (SERS)-encoded this sensing system, a limit of detection of 0.006. pM and a wide linear range of 0.01-10. pM for dopamine detection were obtained. Our work open up a new avenue for the diagnosis and drug-discovery programs. © 2015 Elsevier B.V. Source

Li A.,Academy of Science and Technology of State Administration of Grain | Tang L.,Jiangnan University | Song D.,Academy of Science and Technology of State Administration of Grain | Song S.,Jiangnan University | And 7 more authors.
Nanoscale | Year: 2016

A surface-enhanced Raman scattering (SERS) sensor based on gold nanostar (Au NS) core-silver nanoparticle (Ag NP) satellites was fabricated for the first time to detect aflatoxinB1 (AFB1). We constructed the SERS sensor using AFB1 aptamer (DNA1)-modified Ag satellites and a complementary sequence (DNA2)-modified Au NS core. The Raman label (ATP) was modified on the surface of Ag satellites. The SERS signal was enhanced when the satellite NP was attached to the Au core NS. The AFB1 aptamer on the surface of Ag satellites would bind to the targets when AFB1 was present in the system, Ag satellites were then removed and the SERS signal decreased. This SERS sensor showed superior specificity for AFB1 and the linear detection range was from 1 to 1000 pg mL-1 with the limit of detection (LOD) of 0.48 pg mL-1. The excellent recovery experiment using peanut milk demonstrated that the sensor could be applied in food and environmental detection. © The Royal Society of Chemistry 2016. Source

Peng J.,Jiangnan University | Wang Y.,Academy of Science and Technology of State Administration of Grain | Liu L.,Jiangnan University | Kuang H.,Jiangnan University | And 2 more authors.
RSC Advances | Year: 2016

A new immunochromatographic assay (ICA) was developed for the simultaneous screening of five antibiotics that can coexist in milk, namely lincomycin (LIN), gentamicin (GEN), kanamycin (KAN), streptomycin (STR), and neomycin (NEO), using five corresponding monoclonal antibodies (mAbs). The five mAbs were conjugated to colloidal gold nanoparticles (AuNPs), forming AuNP-labeled antibodies, which were placed in a microtiter well after freeze-drying; the five antigens were immobilized separately on five test lines to align with their corresponding AuNP-labeled antibodies. Using this method, the cutoff values for the strip test in milk were 25 ng mL-1 for LIN, 25 ng mL-1 for GEN, 50 ng mL-1 for KAN, 50 ng mL-1 for STR, and 100 ng mL-1 for NEO, which are below the maximum residue levels set by the European Union. Based on the strip reader, the multiplex strip could detect the concentrations of LIN, GEN, KAN, STR, and NEO as low as 2.5 ng mL-1, 2.5 ng mL-1, 2.5 ng mL-1, 2.5 ng mL-1, and 5 ng mL-1 in milk, respectively. The accuracy and reproducibility of the assay were acceptable when tested on food samples. In conclusion, our ICA strip is useful for the rapid and high-throughout screening of antibiotics in food and environments on-site. © 2016 The Royal Society of Chemistry. Source

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