Time filter

Source Type

Wang W.,Jiangnan University | Wang W.,Academy of Science and Technology of State Administration of Grain | Liu L.,Jiangnan University | Xu L.,Jiangnan University | And 3 more authors.
ACS Applied Materials and Interfaces | Year: 2016

A sensitive surface-enhanced Raman scattering (SERS) immunosensor based on the Au nanoparticle (Au NP) shell structure was developed to detect staphylococcal enterotoxin B (SEB) on a microplate. Au NPs modified with 4-nitrothiophenol (4-NTP) and coated with Ag shell of controlled thickness at 6.6 nm exhibited excellent SERS intensity and were used as signal reporters in the detection of SEB. The engaged 4-NTP allowed the significant electromagnetic enhancement between Au NPs and the Ag shell and prevented the dissociation of the Raman reporter. More importantly, 4-NTP-differentiated SERS signals between the sample and microplate. The SERS-based immunosensor had a limit of detection of 1.3 pg/mL SEB. Analysis of SEB-spiked milk samples revealed that the developed method had high accuracy. Therefore, the SERS-encoded Au@Ag core-shell structure-based immunosensor is promising for the detection of biotoxins, pathogens, and environmental pollutants. © 2016 American Chemical Society.


Tang L.,Jiangnan University | Li S.,Jiangnan University | Han F.,Academy of Science and Technology of State Administration of Grain | Liu L.,Jiangnan University | And 6 more authors.
Biosensors and Bioelectronics | Year: 2015

Dopamine (DA) is a neurotransmitter which plays a key role in the life science. Self-assembled Au@Ag nanorod dimers based on aptamers were developed for ultrasensitive dopamine detection. The electronic field was significantly enhanced by the addition of silver shell coating on the surface of Au NR dimer. The results displayed that Au@Ag NR dimers were ideal building blocks for constructing the SERS substrates with prominent Raman enhancement effects. It was found that with using this Surface-enhanced Raman scattering (SERS)-encoded this sensing system, a limit of detection of 0.006. pM and a wide linear range of 0.01-10. pM for dopamine detection were obtained. Our work open up a new avenue for the diagnosis and drug-discovery programs. © 2015 Elsevier B.V.


Li A.,Academy of Science and Technology of State Administration of Grain | Tang L.,Jiangnan University | Song D.,Academy of Science and Technology of State Administration of Grain | Song S.,Jiangnan University | And 7 more authors.
Nanoscale | Year: 2016

A surface-enhanced Raman scattering (SERS) sensor based on gold nanostar (Au NS) core-silver nanoparticle (Ag NP) satellites was fabricated for the first time to detect aflatoxinB1 (AFB1). We constructed the SERS sensor using AFB1 aptamer (DNA1)-modified Ag satellites and a complementary sequence (DNA2)-modified Au NS core. The Raman label (ATP) was modified on the surface of Ag satellites. The SERS signal was enhanced when the satellite NP was attached to the Au core NS. The AFB1 aptamer on the surface of Ag satellites would bind to the targets when AFB1 was present in the system, Ag satellites were then removed and the SERS signal decreased. This SERS sensor showed superior specificity for AFB1 and the linear detection range was from 1 to 1000 pg mL-1 with the limit of detection (LOD) of 0.48 pg mL-1. The excellent recovery experiment using peanut milk demonstrated that the sensor could be applied in food and environmental detection. © The Royal Society of Chemistry 2016.


Peng J.,Jiangnan University | Wang Y.,Academy of Science and Technology of State Administration of Grain | Liu L.,Jiangnan University | Kuang H.,Jiangnan University | And 2 more authors.
RSC Advances | Year: 2016

A new immunochromatographic assay (ICA) was developed for the simultaneous screening of five antibiotics that can coexist in milk, namely lincomycin (LIN), gentamicin (GEN), kanamycin (KAN), streptomycin (STR), and neomycin (NEO), using five corresponding monoclonal antibodies (mAbs). The five mAbs were conjugated to colloidal gold nanoparticles (AuNPs), forming AuNP-labeled antibodies, which were placed in a microtiter well after freeze-drying; the five antigens were immobilized separately on five test lines to align with their corresponding AuNP-labeled antibodies. Using this method, the cutoff values for the strip test in milk were 25 ng mL-1 for LIN, 25 ng mL-1 for GEN, 50 ng mL-1 for KAN, 50 ng mL-1 for STR, and 100 ng mL-1 for NEO, which are below the maximum residue levels set by the European Union. Based on the strip reader, the multiplex strip could detect the concentrations of LIN, GEN, KAN, STR, and NEO as low as 2.5 ng mL-1, 2.5 ng mL-1, 2.5 ng mL-1, 2.5 ng mL-1, and 5 ng mL-1 in milk, respectively. The accuracy and reproducibility of the assay were acceptable when tested on food samples. In conclusion, our ICA strip is useful for the rapid and high-throughout screening of antibiotics in food and environments on-site. © 2016 The Royal Society of Chemistry.


Kong D.,Jiangnan University | Liu L.,Jiangnan University | Song S.,Jiangnan University | Suryoprabowo S.,Jiangnan University | And 4 more authors.
Nanoscale | Year: 2016

A semi-quantitative and quantitative multi-immunochromatographic (ICA) strip detection assay was developed for the simultaneous detection of twenty types of mycotoxins from five classes, including zearalenones (ZEAs), deoxynivalenols (DONs), T-2 toxins (T-2s), aflatoxins (AFs), and fumonisins (FBs), in cereal food samples. Sensitive and specific monoclonal antibodies were selected for this assay. The semi-quantitative results were obtained within 20 min by the naked eye, with visual limits of detection for ZEAs, DONs, T-2s, AFs and FBs of 0.1-0.5, 2.5-250, 0.5-1, 0.25-1 and 2.5-10 μg kg-1, and cut-off values of 0.25-1, 5-500, 1-10, 0.5-2.5 and 5-25 μg kg-1, respectively. The quantitative results were obtained using a hand-held strip scan reader, with the calculated limits of detection for ZEAs, DONs, T-2s, AFs and FBs of 0.04-0.17, 0.06-49, 0.15-0.22, 0.056-0.49 and 0.53-1.05 μg kg-1, respectively. The analytical results of spiked samples were in accordance with the accurate content in the simultaneous detection analysis. This newly developed ICA strip assay is suitable for the on-site detection and rapid initial screening of mycotoxins in cereal samples, facilitating both semi-quantitative and quantitative determination. © The Royal Society of Chemistry 2016.


Zhang L.,Northeast Agricultural University | Li J.,Northeast Agricultural University | Yun T.T.,Academy of Science and Technology of State Administration of Grain | Qi W.T.,Academy of Science and Technology of State Administration of Grain | And 3 more authors.
Poultry Science | Year: 2015

The effects of microencapsulation of Enterococcus faecalis on the growth performance, antioxidant activity, immune function, and cecal microbiota in broilers were investigated. Broilers (1-day-old) were assigned randomly as follows: 5 treatments, 5 replicate pens per treatment, and 20 broilers per pen. Treatments included (1) a basal diet (CON), (2) CON+Aureomycin (1 g/kg of diet) (ANT), (3) CON+free non-encapsulated probiotics (1×109 cfu/kg of diet) (FREE), (4) CON+proencapsulated probiotics (1×109 cfu/kg of diet) (PRO), and (5) CON+pre-encapsulated probiotics (1×109 cfu/kg of diet) (PRE). Feedings included starter (1 to 21 d) and grower (21 to 42 d) phases. In the starter phase, the ANT and the PRE groups had greater (P <0.05) ADG than the CON groups, and the feed conversion ratio (FCR) for these 2 groups was decreased (P <0.05). In the finisher phase, the PRE and PRO groups had greater (P <0.05) ADG than the CON group and their FCR was decreased significantly (P <0.05). During the entire feeding period, only the PRE group showed greater (P <0.05) ADG and lower (P <0.05) FCR. On day 21, only birds in the PRE group had greater (P <0.05) total antioxidant capacity and number of Lactobacillus than the CON group. On day 42, The PRE group showed greater (P <0.05) superoxide dismutase than the CON group. Serum IgA and IgM concentrations were increased (P <0.05) in the PRE group. Serum IL-6 in the PRE group was greater (P <0.05) than in the other groups with the exception of ANT. At the phylum level, Firmicutes was enriched (P <0.05) and Proteobacteria was depleted (P <0.05) only in the PRE group. At the genus level, only the PRE diets increased (P <0.05) the number of both Lactobacillus and Enterococcus. The results indicate that pre-encapsulation assists the efficient functioning of probiotics in broilers. © 2015 Poultry Science Association Inc.


Zhang L.,Northeast Agricultural University | Li J.,Northeast Agricultural University | Yun T.T.,Academy of Science And Technology Of State Administration of Grain | Li A.K.,Academy of Science And Technology Of State Administration of Grain | And 4 more authors.
Journal of Animal Science | Year: 2015

This study was conducted to evaluate the pilot-scale production of microencapsulated Saccharomyces boulardii in a 500-L fermenter using emulsion and gelation and to assess the effect of the products on the growth performance, antioxidant activity, immune function, and cecal microbiota in Arbor Acres broilers. A total of seven hundred 1-d-old male Arbor Acres broilers were randomly assigned to 7 dietary treatments with 5 replicate pens per treatment and 20 broilers per pen. The dietary treatments were as follows: 1) basal diet (CON), 2) basal diet containing 0.1% Aureomycin (ANT), 3) basal diet containing unencapsulated S. boulardii at a dose of 1 × 108 cfu/kg of feed (P1), 4) basal diet containing unencapsulated S. boulardii at a dose of 1 × 1010 cfu/kg of feed (P2), 5) basal diet containing 0.01% empty microcapsules (CAP), 6) basal diet containing microencapsulated S. boulardii at a dose of 1 × 108 cfu/kg of feed (CAPP1), and 7) basal diet containing microencapsulated S. boulardii at a dose of 1 × 1010 cfu/kg of feed (CAPP2). The feeding experiment included 2 phases: the starter phase from d 1 to 21 and the grower phase from d 22 to 42. The results showed that a 500-L fermenter could produce 20.73 ± 4.05 kg of microcapsules with an approximate diameter of 549 μm. The feeding experiment showed that ADG of broilers in CAPP1 was significantly (P < 0.05) greater than that in CON and CAP throughout the feeding period, whereas the ratio of feed to gain (G: F) was significantly (P < 0.05) lower. Broilers in P1, P2, CAPP1, and CAPP2 had significantly (P < 0.05) greater levels of total superoxide dismutase, catalase, IgG, and cluster of differentiation 3 than those in CON. Furthermore, broilers in CAPP1 had significantly (P < 0.05) greater richness and diversity of intestinal microorganisms, particularly of Lactobacillus, than those in all other dietary treatments. In summary, our results indicate that large-scale microencapsulation of microbial cells can be achieved using emulsion and initial gelation and that the dietary administration of microencapsulated S. boulardii can significantly enhance the growth performance, immune function, cecum microbial community, and overall health of broilers. © 2015 American Society of Animal Science. All rights reserved.


Dong Z.L.,Wuhan Polytechnic University | Wang Y.W.,Academy of Science and Technology of State Administration of Grain | Song D.,Academy of Science and Technology of State Administration of Grain | Hou Y.J.,Academy of Science and Technology of State Administration of Grain | And 4 more authors.
Animal Feed Science and Technology | Year: 2016

The objective of this study was to investigate the effects of microencapsulated Enterococcus fecalis (MEF) and the extract of Camellia oleifera seed (ECOS) on growth performance, morphological parameters and gene expressions related to barrier functions of the intestinal mucosa in broiler chickens. 160-day-old Arbor Acres male broilers were randomly allotted to 4 treatments with 8 replicates of 5 chicks in a 2. ×. 2 factorial arrangement, evaluating two levels of MEF (0 and 10. g/kg), and two levels of ECOS (0 and 300. mg/kg). The results showed that there was no significant interaction effect of MEF and ECOS on growth performance, morphometric parameters of jejunum and ileum, and mRNA expression of MUC2, occludin and claudin-1 in jejunum and ileum mucosa. Compared with other groups, diet supplemented with MEF significantly improved FCR from days 21 to 42, and significantly improved ADG from days 0 to 42. Dietary MEF significantly increased villus height and villus height to crypt depth ratio in jejunum and ileum, the concentrations of sIgA, IL-2, IL-6, TNF-α, and significantly up-regulated the gene expression of sIgA, MUC2, occludin and claudin-1 in jejunum and ileum mucosa. The interaction effect of MEF and ECOS was significant on the concentrations of sIgA, IL-2, IL-6, TNF-α in jejunum mucosa on day 42 and on the concentrations of sIgA, IL-2, TNF-α in ileum mucosa on day 42. These results indicated that MEF can improve growth performance and enhance intestinal barrier functions by improving its morphological development, increasing cytokine level, and up-regulating the gene expressions of sIgA, MUC2, occludin and claudin-1. But ECOS had slightly beneficial effects on growth performance and intestinal barrier functions. © 2015.

Loading Academy of Science and Technology of State Administration of Grain collaborators
Loading Academy of Science and Technology of State Administration of Grain collaborators