Jin M.-L.,Academy of Military Medical of PLA |
Lu H.-J.,Academy of Military Medical of PLA |
Ma M.-X.,Academy of Military Medical of PLA |
Zheng M.,Academy of Military Medical of PLA |
And 2 more authors.
Chinese Journal of Biologicals | Year: 2010
Objective: To determine the distribution and toxicity of fowlpox virus (FPV) in pigeon. Methods: Pigeons were inoculated with FPV at skin of pectoral wall, 50 μl for each, using physiological saline as control, and observed for clinical signs daily. Partial pigeons were killed 6 h, 12 h, 1 d, 3 d, 7 d, 10 d, 15 d and 21 d after inoculation respectively, observed for histopathology in various organs and determined for distribution of FPV by PCR. Results: During the observation, the mental status, ingestion and water intake of pigeons were normal, while the clinical signs disappeared basically 15 d after inoculation. No significantly pathogenic changes were observed in various tissues. Viral DNA was detected in the muscle in inoculation site 6 h, in all the tissues 3 d and 7 d and in muscle and spleen 10 d after inoculation. However, no viral DNA was detected in any tissues 15 d after inoculation. All the detection results of viral DNA were negative in control group during the whole study. Conclusion: In pigeons, FPV existed for a short time and showed no toxicity, indicating good biosafety.