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Tuteja S.K.,Academy of Innovative and Scientific Research AcSIR CSIO | Tuteja S.K.,CSIR - Central Electrochemical Research Institute | Kukkar M.,Academy of Innovative and Scientific Research AcSIR CSIO | Kukkar M.,Sri Guru Granth Sahib World University | And 4 more authors.
BioNanoScience | Year: 2014

Metal enhanced fluorescence assay-based sensitive detection of a neurotoxic organophosphates pesticide, Paraoxon, is reported. The experimental approach involves the bio-interfacing of organophosphorus hydrolase with a high quantum yield fluorophore, pyranine (8-hydroxyl pyrene-1,3,6-trisulfonic acid trisodium salt), followed by the conjugation of the OPH-pyranine derivative with silica-coated silver nanoparticles (Ag NPs). The above bio-nanoprobe was used for the analysis of the organophosphate pesticide, wherein the induced hydrolysis of the pesticide cause the decrease in pH in the vicinity. An excitation light of 460 nm wavelength was used to monitor the changes in the resulting emission (at 510 nm) with respect to the changing pesticide concentrations (1-100 ppb). The introduction of the silica-coated AgNPs into the nanoprobe system was observed to deliver metal enhanced effect, leading to almost10-fold intensification of the fluorescence signal. The enhanced fluorescence assay format offers linear detection of Paraoxon in the concentration range of 1-100 ng mL-1 with the limit of detection 1 ng mL-1 (1 ppb). © 2014 Springer Science+Business Media New York.


Singh S.,CSIR - Central Electrochemical Research Institute | Singh S.,Academy of Innovative and Scientific Research AcSIR CSIO | Tuteja S.K.,CSIR - Central Electrochemical Research Institute | Tuteja S.K.,Academy of Innovative and Scientific Research AcSIR CSIO | And 4 more authors.
Microchimica Acta | Year: 2016

A composite consisting of gold nanoparticles and reduced graphene oxide (AuNPs@rGO) was electrochemically prepared in-situ on a screen printed electrode (SPE) which then was used as an immunosensor for the cardiac biomarker myoglobin. The nanocomposite was characterized by transmission electron microscopy (TEM, scanning electron microscopy (SEM), atomic force microscopy, FTIR and electrochemical impedance spectroscopy. For FTIR, TEM and SEM, the deposition was done on indium tin oxide coated glass plates. The immunosensor was obtained by immobilization of in-house generated antibody against cardiac myoglobin on the electrode surface. The immunosensing response was monitored using differential pulse voltammetry, which showed a reduction peak at ~ −0.5 V (vs. Ag/AgCl). The reduction peak arises from the reduction of iron moiety present in the heme group of myoglobin. The immunosensor exhibited dynamic linearity range from 1 ng.mL−1 to 1400 ng.mL−1 with the detection limit of ~0.67 ng.mL−1 for cardiac myoglobin. The obtained result was almost eight times better (in terms of detection limit) than that obtained with ELISA tests (with detection limit of ~4 ng.mL−1) using the same antibodies. The immunosensor was applied to analyze spiked serum samples also. [Figure not available: see fulltext.] © 2016 Springer-Verlag Wien


Tuteja S.K.,Academy of Innovative and Scientific Research AcSIR CSIO | Tuteja S.K.,CSIR - Central Electrochemical Research Institute | Tuteja S.K.,Chandigarh Institute of Microbial Technology | Kukkar M.,Academy of Innovative and Scientific Research AcSIR CSIO | And 6 more authors.
Biosensors and Bioelectronics | Year: 2015

2-Aminobenzyl amine (2-ABA) functionalized graphene is proposed for the ultrasensitive immunosensing of Cardiac Troponin I (cTnI). 2-ABA was electrochemically polymerized on the graphene decorated interdigitated electrode to obtain the amine functionalized graphene (. f-GN). The f-GN electrode was then modified with monoclonal anti-cTnI antibodies via Schiff reaction based chemistry. Detailed characteristics of the processes involved and the finally developed antibody conjugated f-GN interdigitated electrode have been studied. The above micro-device was used in a drain source configuration for the sensing of cTnI. A wide dynamic linear range of antigen detection (0.01-1. ng/mL) is achieved with the limit of detection of 0.01. ng/mL. The utility of the proposed sensing technique is demonstrated by successfully testing the antigen concentration in spiked serum samples. © 2014 Elsevier B.V.

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