Academy of Chinese Medicine and Material Medica of Jilin Province

Changchun, China

Academy of Chinese Medicine and Material Medica of Jilin Province

Changchun, China
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Wang Y.,South China University of Technology | Gao Q.-P.,Changchun University of Chinese Medicine | Li G.-R.,Yanbian University | Chen Y.-H.,Academy of Chinese Medicine and Material Medica of Jilin Province | And 3 more authors.
Chemical Research in Chinese Universities | Year: 2011

A mixture of oligosaccharide and glycopeptide was isolated from the aqueous extract of Panax ginseng roots. The mixture inhibits protein tyrosine phosphatase(SHP-1) function, implying it enhances immune activity. The peak molecular mass of the oligosaccharide portion is 1800 calculated via GPC software after separation by HPLC. And the structure of the oligosaccharide portion is the backbone of (1→3)- and (1→4)-linked arabinopyranoside, and (1→4)- and (1→6)-linked glucopyranoside, with non-reducing terminals of arabinopyranoside and glucopyranoside. The peak molecular mass of glycopeptide portion is 1900 calculated via GPC software after separation by HPLC. The structure of glycopeptide portion is the backbone of (1→3)- and (1→4)-linked arabinopyranoside, and (1→3,6)-linked glucopyranoside, with non-reducing terminals of galactopyranose and glucopyranoside. The peptide composition is Glu, Asp, Hyp, Ser, Arg, Gly, Thr, Pro, Ala, Val, Ile, Leu and Lys. The oligosaccharide-peptide linkage is formed by Ara and Hyp.


Wang Y.,South China University of Technology | Jiang R.,Academy of Chinese Medicine and Material Medica of Jilin Province | Li G.,Yanbian University | Chen Y.,Academy of Chinese Medicine and Material Medica of Jilin Province | And 3 more authors.
Food Chemistry | Year: 2010

The aim of this paper is to study low molecular mass carbohydrate polymer from Panax ginseng root. The aqueous extracts of P. ginseng root were loaded onto a macroporous resin, then forced through a Cellulose Super Filtration System, and loaded onto a Carbon-Celite column and eluted with water, 5% ethanol, 30% ethanol and 95% ethanol. To determine if 30% ethanol elution from a Carbon-Celite column could enhance memory in scopolamine-induced memory deficit rats, Mirros water maze task was used. The purification of the 30% ethanol elution was performed on Sephadex G-15 and Bio-gel P2 columns. GC-MS analysis of methylated derivatives as well as ESI-MS measurement were used for structural confirmation. Results showed that 30% ethanol elution could enhance memory in scopolamine-induced memory deficit rats with intraperitoneal administration. Oligosaccharides and peptides were isolated from 30% ethanol elution. The oligosaccharides were identified as maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose. © 2009 Elsevier Ltd. All rights reserved.

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