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Wang D.,Key Laboratory of Molecular and Cytogenetics | Wang D.,Harbin NormalUniversity | Yu Y.,Key Laboratory of Molecular and Cytogenetics | Yu Y.,Harbin NormalUniversity | And 3 more authors.
2010 4th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2010 | Year: 2010

To analyze the biological effects of space flight on pepper seed, the cytological effects of SP1, SP2, and SP3 generations of pepper ILT-31 lines after space flight were studied. Cytogenetic analyses were performed as the frequency of chromosomal aberrations assay and mitotic index (MI). The results showed that the space environment can increase the mitotic index, and induce different chromosomal aberrations including micronuclei, chromosomal bridges, fragments, laggards, and multipolar division etc. The highest mitotic index and rate of chromosomal aberrations were found in III-31-SP2(P<0.01). © 2010 IEEE. Source

Wu J.,Academy of Agriculture Science | Wu J.,Xinjiang Shihezi Vocational Technical College | Gao X.,Seismological Bureau of Xinjiang Uygur Autonomous Region | Zeng J.,Xinjiang University | And 3 more authors.
Shengtai Xuebao/ Acta Ecologica Sinica | Year: 2011

Seismic fault belt is a main passageway of various kinds of gas and geochemistry elements, where are important zone for matters, energy and information transfer between lithosphere and atmosphere. Microbes living in the belt effected intensively by the hydrogeochemical changes caused by earth crust movement. The 10th spring of Urumq originates from Bogeda mountain of Tianshan mountain range, and the groundwater runoff in seismic fault belt. The spring water contains various geochemistry elements, such as sulfide, methane, hydrogen, radon, sulfide, methane, carbon dioxide, helium, fluorine and hydrarg et al, many of which may potentially act as electron donors capable of supporting chemolithotrophy-based primary production. In order to reveal the impact of hydrogeochemical changes to bacterial community in seismic fault belt spring water, thirty-one samples of spring water collected from October 12th to November 11th and their corresponding geochemial parameters were determined. Of them, ten samples were used for bacterial dynamic changing monitoring. The planktonic communities were collected by microporous membrane filtration and then total DNA were extracted by SDS-enzymatic disruption method. The V3 region of the 16S rDNA gene of those samples was analyzed by PCR-DGGE (Denaturing Gradient Gel Electrophoresis) and different bands were excised for sequencing. The DGGE fingerprints combined with hydrogeochamical parameters were analyzed by CCA (Canonical correspondence analysis). The results showed that B16 (Uncultured bacterium) and epsilon proteobacterium were positive correlate to Fluorine fluctuation and clustered as group one and two, respectively; B1 (Flavobacterium), Pseudomonas aeruginosa, B10 (Uncultured bacterium) and Staphylococcus saprophyticus were positive correlate to sulfide change; Thiomicrospira arctica,B3 (Flavobacterium)aand Staphylococcus arlettae were positive correlated to hydrogen content. The result indicated that bacterial communities in seismic fault belt spring water could response sensitively to hydrogeochemical parameter changes, and this may provide a novel means for crust movement monitoring and earthquake forecast. Source

Zheng W.,Academy of Agriculture Science | Ma L.,Shenyang University | Zhao J.,Academy of Agriculture Science | Li Z.,Academy of Agriculture Science | And 2 more authors.
PLoS ONE | Year: 2013

Rice stripe, a virus disease, transmitted by a small brown planthopper (SBPH), has greatly reduced production of japonica rice in East Asia, especially in China. Although we have made great progress in mapping resistance genes, little is known about the mechanism of resistance. By de novo transcriptome assembling, we gained sufficient transcript data to analyze changes in gene expression of early interaction in response to SBPH and RSV infection in rice. Respectively 648 and 937 DEGs were detected from the disease-resistant (Liaonong 979) and the susceptible (Fengjin) varieties, most of which were up-regulated. We found 37 genes related to insect resistance, which mainly included genes for jasmonate-induced protein, TIFY protein, lipoxygenase, as well as trypsin inhibitor genes and transcription factor genes. In the interaction process between RSV and rice, 87 genes were thought to be related to RSV resistance; these primarily included 12 peroxidase biosynthesis genes, 12 LRR receptor-like protein kinase genes, 6 genes coding pathogenesis-related proteins, 4 glycine-rich cell wall structural protein genes, 2 xyloglucan hydrolase genes and a cellulose synthase. The results indicate that the rice-pathogen interaction happened both in disease-resistant and susceptible varieties, and some genes related to JA biosynthesis played key roles in the interaction between SBPHs and rice. When rice was infected by RSV a hypersensitive reaction (HR) in the disease-resistant variety was suppressed, which resulted from an increase in peroxidase expression and down-regulation of LRR receptor-like protein kinase and pathogenesis-related proteins, while, the changes of peroxidase biosynthesis, glycine-rich cell wall structural protein, cellulose synthase and xyloglucan endotransglucosylase/hydrolase could lead to the strengthening of physical barriers of rice, which may be an important resistance mechanism to RSV in rice. © 2013 Zheng et al. Source

Zheng W.,Academy of Agriculture Science | Li Z.,Academy of Agriculture Science | Zhao J.,Academy of Agriculture Science | Zhang Y.,Academy of Agriculture Science | And 3 more authors.
Pest Management Science | Year: 2016

BACKGROUND: The small brown planthopper (Laodelphax striatellus) is a widespread insect pest of rice in East Asia. Previous studies have shown the long-distance migrations undertaken by L. striatellus, but have not provided molecular evidence to support this. RESULTS: Long-distance immigration has occurred in the north-east coastal rice-growing region of China. Using the specific-locus amplified fragment sequencing technique, sequence data for 2.7 Gb of an abruptly increased population and 13 L. striatellus local populations from a range of regions in China that have serious rice stripe disease were obtained. A total of 2572 single nucleotide polymorphisms (SNPs) and 37 indels were detected, and the genotypes of many polymorphism sites were heterozygous in every sample, which indicated that there were rich genetic differences among the populations, and that the migration of insect pests accelerated the gene flow and increased the heterozygosity of L. striatellus populations. The genetic distance and the polymorphism markers among different populations showed that the abruptly increased population in Liaoning Province is close to several populations from Jiangsu Province and Shandong Province. CONCLUSION: The vector that caused rice stripe disease in the north-east of China was an immigrant population; however, the population may be formed from several groups from different areas, such as Jiangsu and Shandong provinces. © 2015 Society of Chemical Industry © 2016 Society of Chemical Industry. Source

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