Academic Center for Education Culture and Research

Arāk, Iran

Academic Center for Education Culture and Research

Arāk, Iran

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Kazemizadeh Z.,Academic Center for Education Culture and Research | Habibi Z.,Shahid Beheshti University | Yousefzadi M.,Academic Center for Education Culture and Research | As'habi M.A.,Urmia University
Journal of Medicinal Plants | Year: 2010

Background: In the Flora Iranica this genus is represented by fifthy-eight species, seventheen of which are endemic. The genus Salvia are known for medicinal value, antibacterial and antioxidant properties. Objective: Investigation on essential oil of Salvia macrochlamys and antibacterial activity assessment. Methods: The aerial parts of S. macrochlamys were collected during the flowering stage from Marmishuo Hill in West-Azarbayjan Province, in July 2006 and were hydrodistilled for 3 hours, using a Clevenger-type apparatus to yield 0.35% (w/w) of yellowish oil and analyzed with GC and GC/MS. The constituents of the essential oil were identified by comparison of their mass spectra and retention indices (RI) with those given in the literature and authentic samples. Antibacterial activities of the essential oil were investigated according to the disc diffusion method and MIC value. Results: Thirty-four compounds were characterized in the essential oil of S. macrochlamys, representing 97.6% of the oil, of which β-Caryophyllene (32.7%), 1,8-Cineol (18.9%), were found to be the major components. The oil of S. macrochlamys showed high antibacterial activity against Bacillus subtilis, Staphylococcus aureus and Staphylococcus epidermidis. Conclusion: The oil of S. macrochlamys consists of monoterpen (44.4%) and sequiterpens (52.0%). The antibacterial avtivity can be attributed to 1,8-Cineol.


Kazemizadeh Z.,Academic Center for Education Culture and Research | Habibi Z.,Shahid Beheshti University
Journal of Medicinal Plants | Year: 2010

Background: The genus Teucrium (Lamiaceae) is comprised 340 species in widespread over the world. Remarkably, In the Flora Iranica this genus is represented by twelve species, three of which are endemic. The genus Teucrium are known for medicinal value, antibacterial, antioxidant, anti-inflamatory, antiseptic and antipyretic properties. Objective: Isolation of β-Stigmasterol from Teucrium hyrcanicum L. Methods: The aerial parts of Teucrium hyrcanicum were collected during the flowering stage from Rostamabad, Gilan Province, in July 2006 and then were extracted by chloroform and acetone (2:1) for 36 h. Crude extract was separated by adsorption chromatography over silica gel with elution by petroleum and ethylacetate. Final purification was performed by smaller chromatography. Results: A steroid named β - Stigmasterol were isolated and the structur of them were proved using spectral data consist of FTIR, UV, HRMS, also 1-DNMR ( 1HNMR, 13CNMR), 2-DNMR (HMQC, HMBC) and DEPT (90°,135°). Conclusion: β-Stigmasterol was characterized for the first time in Teucrium hyrcanicum.


Saien J.,Bu - Ali Sina University | Shahrezaei F.,Academic Center for Education Culture and Research
International Journal of Photoenergy | Year: 2012

A real petroleum refinery wastewater, containing a range of aliphatic and aromatic organic compounds, was treated using nanotitania particles, as the photocatalyst in UV/TiO 2 process. Samples were collected from the inlet point of the biological treatment unit. A conic-shape, circulating, and upward mixing reactor, without dead zone, was employed. The light source was an immersed mercury UV lamp (400W, 200550nm). Optimal suspended catalyst concentration, fluid pH, and temperature were obtained at amounts of near 100 mg L. © 2012 I. Salas-Garca et al.


Hafezi M.,Iranian Materials and Energy Research Center | Reza Talebi A.,University of Yazd | Mohsen Miresmaeili S.,Academic Center for Education Culture and Research | Sadeghian F.,University of Yazd | Fesahat F.,University of Yazd
Ceramics International | Year: 2013

There is a conspicuous need for in vivo evaluation of biomaterials prior to their application as bone graft or tissue engineering scaffold. Merwanite and HA in vivo application as bone filler would be studied in this study. Femoral injury was induced in male rats and early and late degrees in bone repair were evaluated. A histomorphological analysis of the bioceramics implants in rat femoral defect models suggested that both in early and late stage of bone repair, merwinite is more effective in promoting osteogenesis in comparison with HA and in late stage, the rate of new bone formation was faster in merwinite-filled-bone-defect than in HA models. The control groups showed limited osteogenesis. These results suggested that merwinite might be a potential and attractive bioceramic for bone replacement. © 2012 Elsevier Ltd and Techna Group S.r.l.


Bahrambeigi V.,Isfahan University of Medical Sciences | Bahrambeigi V.,Academic Center for Education Culture and Research | Ahmadi N.,Isfahan University of Medical Sciences | Ghafarizadeh A.A.,Academic Center for Education Culture and Research | And 3 more authors.
Gene Therapy and Molecular Biology | Year: 2013

Murine mesenchymal stem cells (MSCs) are common tools in gene therapy experiments. In this study, we measured, optimized, and compared transfection efficiency of different non-viral methods into murine adipose derived MSCs (AD-MSCs). AD-MSCs were isolated from fat tissue of C57BL6 mice. Authenticity and surface markers expression of murine AD-MSCs were assessed. Transfection yields of different transfectants along with nucleofection in different conditions or programs were determined and compared. The isolated AD-MSCs were successfully differentiated into three lineages. AD-MSCs were positive for CD73, CD90, CD105, negative for CD34 and CD45 and lowly expressed CD24. The best transfection rates were 18.14 ± 3.41% (ExpressIn), 24.64 ± 4.37% (GenCarrier-2), 11.43 ± 3.17% (GeneTranTMII), 26.4 ± 4.17% (LipofectaminTM 2000), 11.51 ± 1.97% (MesenFectagen), 29.49 ± 5.31% (TransIT®-2020), and 73.62 ± 6.81 (nucleofection; with ~41% of toxicity). After selecting an appropriate method, we used PhiC31 (φC31) integrase system to overcome the problem of transient gene expression. Although, we reached only moderate transfection rates using transfection reagents, nucleofection was the most efficient method for gene transfer into murine AD-MSCs. Results also indicated that stably transfected AD-MSCs can be generated after φC31 integrase-mediated transgene integration of nucleofected AD-MSCs. Our results show non-viral gene delivery can be used for efficient, safe, and stable modification of MSCs.


Sahari M.A.,Tarbiat Modares University | Farahani F.,Academic Center for Education Culture and Research | Soleimanian Y.,Tarbiat Modares University | Mokhlesi A.,Academic Center for Education Culture and Research
JAOCS, Journal of the American Oil Chemists' Society | Year: 2013

For application of n-3 fatty acids, distribution of the fatty acid compositions of different parts of (head, tail, fins and skin = HTFS, liver, viscera and muscle tissue) five commercially important fish species from the Persian Gulf (Scomberomorus commersoni, Thunnus tonggol, Euthynnus affinis, Scomberomorus guttatus and Dussumieria acuta) as good sources of n-3 PUFA were studied. The richest source of n-3 were HTFS in S. guttatus and S. commersoni, liver in S. guttatus, total body of D. acuta, liver of E. affinis and T. tonggol, followed by viscera of E. affinis. The content of these fatty acids were the same in viscera of tonggol, liver of S. commersoni, and HTFS of E. affinis. Moreover, muscle of E. affinis and HTFS of T. tonggol and also muscle of S. guttatus and T. tonggol had the same n-3 contents as the viscera of S. commersoni. So, it was concluded that HTFS and viscera (which are discarded as residues) are as useful as muscle and liver and can be a source of economically available n-3 PUFA. Muscle had the lowest proportion of n-3 in E. affinis, T. tonggol, and S. guttatus in comparison with other organs of these fish species. The highest n-3:n-6 ratio was observed in D. acuta. Finally, the cluster analysis showed that with respect to n-3 and other PUFA contents, HTFS of S. commersoni and D. acuta with S. guttatus on the one hand, and HTFS of T. tonggol and E. affinis on the other hand were similar to each other. In addition, viscera of S. commersoni and S. guttatus were similar followed by T. tonggol and different from E. affinis and D. acuta. In the case of muscle, T. tonggol and S. guttatus had good similarity followed by E. affinis and had no significant similarity with S. commersoni and D. acuta. With regard to liver, the highest similarity was observed between T. tonggol and E. affinis followed by D. acuta and S. guttatus, while S. commersoni did not show similarity with the others. © 2013 AOCS.


Kazemizadeh Z.,Academic Center for Education Culture and Research | Moradi A.,Agricultural and Natural Resources Research Center of Iran | Yousefi M.,Shahid Beheshti University
Journal of Medicinal Plants | Year: 2011

Background: The genus Achillea (Asteraceae) is comprised of about 115 species in over the world. Remarkably, 19 species of this genus are found in the flora Iranica. This genus has been used in folk medicine. Objective: Investigation of the essential oil from leaf and flowers of Achillea nobilis L. subsp. neireichii. Methods: The aerial parts of A. nobilis L., subsp. neireichii were collected during the flowering stage from Damash village, West of Roodbar, Gilan Province, in July 2007. Leaves and flowers were separately hydrodistilled for 3 hours, using a Clevenger-type apparatus to yield 1.8% (w/w) and 1.0% (w/w) of yellow oil, respectively and analyzed with GC and GC/MS. The constituents of the essential oil were identified by comparison of their mass spectra and retention indices (RI) with those given in the literature, co-injection and authentic samples. Results: Forthy-four (86.8%) and forthy - two (93.4%) compounds were characterized in the oil of flowers and leaves of A. nobilis subsp. neireichii, respectively. 1,8-cineole (10.3%) and geranyl isovalerate (8.4%) were the major constituents in the oil of the flowers, also 1,8-Cineole (17.0%) and trans-verbenol (14.1%) were the major constituents in the oil of leaves. Conclusion: The oil of flowers and leaves of A. nobilis subsp. neireichii consist of monoterpens (59.8% and 72.2%) and sesquiterpens (22.2% and 12.6%), respectively.


Mahdian S.,Islamic Azad University | Mahdian S.,Academic Center for Education Culture and Research | Aflatoonian R.,Academic Center for Education Culture and Research | Yazdi R.S.,Royan Institute for Reproductive Biomedicine | And 4 more authors.
Fertility and Sterility | Year: 2015

Objective: To evaluate the expression of MIF, CD74, and COX-2 in normal, ectopic, and eutopic endometrium during the menstrual cycle and to assess MIF level in peripheral blood. Design: The expressions of MIF, CD74, and COX-2 in normal, ectopic, and eutopic endometrium were evaluated with the use of realtime polymerase chain reaction. MIF protein in peripheral blood samples was checked with the use of ELISA. Setting: Reproductive biomedicine research center. Patient(s): Sixteen normal women and 20 women with endometriosis. Intervention(s): Ectopic biopsies were obtained with the use of laparoscopic procedure, and eutopic and control biopsies were obtained with the use of Pipelle. Peripheral blood samples were collected before laparoscopy. Main Outcome Measure(s): The expression of MIF, CD74, and COX-2 in normal, ectopic and eutopic endometrium during the menstrual cycle and the expression level of MIF in peripheral blood samples. Result(s): Relative mRNA expression of MIF, CD74, and COX-2 were significantly higher in ectopic endometrium than in eutopic and control endometrium. Also, there were significant differences in expression of these genes in normal, ectopic, and eutopic endometrium during the menstrual cycle. Moreover, women with endometriosis had significantly higher circulating levels of MIF compared with control subjects. Conclusion(s): Dynamic expression of MIF, CD74 , and COX-2 during the menstrual cycle could play an essential role in reproduction, inflammation, and endometrium reconstruction. A higher expression of these genes in ectopic endometrium can be considered as a molecular biomarker for endometriosis development and pathophysiology. Also, a high level of MIF in blood serum can act as a biomarker in the diagnosis of endometriosis. ©2015 by American Society for Reproductive Medicine.


Shahmiri M.,University Putra Malaysia | Ibrahim N.A.,University Putra Malaysia | Shayesteh F.,National University of Malaysia | Asim N.,National University of Malaysia | Motallebi N.,Academic Center for Education Culture and Research
Journal of Materials Research | Year: 2013

Copper oxide (CuO) nanosheets synthesized in polyvinylpyrrolidone (PVP) were characterized with respect to antimicrobial activity by quick precipitation method. Different sizes and shapes of CuO nanosheets were obtained by simple variations of PVP concentrations. The x-ray diffraction results revealed the formation of pure-phase CuO with monoclinic structure. Transmission electron microscopy analysis showed that the average ratio of length to width of these nanosheets increased with increasing PVP concentrations. Due to the quantum size effect, CuO nanosheets exhibit a blue shift in the ultraviolet-visible spectra. Field emission scanning electron microscopy results showed that as the concentration of PVP increased, well-defined morphologies were formed on the surface of the products. Energy dispersive analysis of x-ray clearly confirmed the presence of Cu and O with an atomic ratio of 1:1. Fourier transform infrared spectroscopy results showed that C=O in PVP coordinated with CuO and formed a protective layer. The mechanism of the reaction was also discussed. CuO nanosheets in suspension showed activity against a range of bacterial pathogens and fungi with minimum bactericidal concentrations (MBCs) ranging from 100 to 5000 μ/mL. The extent of the inhibition zones and the MBCs was found to be size-dependent. Copyright © Materials Research Society 2013.


Sahari M.A.,Tarbiat Modares University | Farahani F.,Academic Center for Education Culture and Research | Soleimanian Y.,Tarbiat Modares University | Javadi A.,Academic Center for Education Culture and Research
Journal of Applied Ichthyology | Year: 2014

In the current study, the effect of frozen storage at -18°C was evaluated on fatty acid composition of different body parts (liver, muscle tissue, and viscera) of narrow-barred Spanish mackerel (Scomberomorus commerson, Lacépède, 1800), longtail tuna (Thunnus tonggol, Bleeker, 1851), kawakawa (Euthynnus affinis, Cantor, 1849), king mackerel (Scomberomorus guttatus, Bloch & Schneider, 1801), and rainbow sardine (Dussumieria acuta, Valenciennes, 1847) caught in the Persian Gulf. Changes in saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs), polyunsaturated fatty acids (PUFAs), eicosapentaenoic acid plus docosahexaenoic acid/palmitic acid (EPA+DHA/C16), ω3 PUFA/ω6 PUFA (ω3/ω6), and polyunsaturated fatty acids/saturated fatty acids (PUFA/SFA) were investigated during a 6-month period. A decrease in unsaturated fatty acids, particularly PUFAs (60-100%) as well as ω3/ω6, EPA+DHA/C16 (polyene index) and PUFA/SFA ratios, indicated a decrease in the nutritional values of the samples.

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